Comparison of the in vivo antimicrobial effectiveness of sodium hypochlorite and chlorhexidine used as root canal irrigants: A molecular microbiology study

Journal of Endodontics

Volumn 37, Issue 2, 2011, Pages 143-150

  Rôças, Isabela N ^a   Siqueira Jr , José F ^a  

^a ESTÁCIO DE SÁ UNIVERSITY   (Brazil)

Author keywords

Apical periodontitis; checkerboard DNA DNA hybridization; chlorhexidine; endodontic treatment; polymerase chain reaction; sodium hypochlorite

Indexed keywords

BIOMEDICAL AND DENTAL MATERIALS; CHLORHEXIDINE; DISINFECTANT AGENT; HYPOCHLORITE SODIUM;

ARTICLE; BACTERIUM; CHEMISTRY; CLASSIFICATION; CLINICAL TRIAL; COMPARATIVE STUDY; DRUG EFFECT; HUMAN; MICROBIOLOGY; TOOTH PERIAPICAL DISEASE; TOOTH PULP; TREATMENT OUTCOME;

BACTERIA; CHLORHEXIDINE; DENTAL PULP CAVITY; DISINFECTANTS; HUMANS; PERIAPICAL PERIODONTITIS; ROOT CANAL IRRIGANTS; SODIUM HYPOCHLORITE; TREATMENT OUTCOME;

EID: 78751494215     PISSN: 00992399     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joen.2010.11.006     Document Type: Article

References (40)

1. D. Ricucci, and J.F. Siqueira Jr. Biofilms and apical periodontitis: study of prevalence and association with clinical and histopathologic findings J Endod 36 2010 1277 1288
2. J.F. Siqueira Jr., and I.N. Rôas Clinical implications and microbiology of bacterial persistence after treatment procedures J Endod 34 2008 1291 1301
3. A. Byström, and G. Sundqvist Bacteriologic evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy Oral Surg Oral Med Oral Pathol 55 1983 307 312
4. J.F. Siqueira Jr., I.N. Rôas, and A. Favieri Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite J Endod 26 2000 331 334
5. P.R. Brito, L.C. Souza, and J.C. Machado de Oliveira Comparison of the effectiveness of three irrigation techniques in reducing intracanal Enterococcus faecalis populations: an in vitro study J Endod 35 2009 1422 1427
6. M. Zehnder Root canal irrigants J Endod 32 2006 389 398
7. P. Ohara, M. Torabinejad, and J.D. Kettering Antibacterial effects of various endodontic irrigants on selected anaerobic bacteria Endod Dent Traumatol 9 1993 95 100
8. J.F. Siqueira Jr., M.M. Batista, and R.C. Fraga Antibacterial effects of endodontic irrigants on black-pigmented gram-negative anaerobes and facultative bacteria J Endod 24 1998 414 416
9. M.E. Vianna, H.P. Horz, and B.P. Gomes In vivo evaluation of microbial reduction after chemo-mechanical preparation of human root canals containing necrotic pulp tissue Int Endod J 39 2006 484 492
10. A.M. Ringel, S.S. Patterson, and C.W. Newton In vivo evaluation of chlorhexidine gluconate solution and sodium hypochlorite solution as root canal irrigants J Endod 8 1982 200 204
11. F.R. Dametto, C.C. Ferraz, and B.P. de Almeida Gomes In vitro assessment of the immediate and prolonged antimicrobial action of chlorhexidine gel as an endodontic irrigant against Enterococcus faecalis Oral Surg Oral Med Oral Pathol Oral Radiol Endod 99 2005 768 772
12. M.M. Menezes, M.C. Valera, and A.O. Jorge In vitro evaluation of the effectiveness of irrigants and intracanal medicaments on microorganisms within root canals Int Endod J 37 2004 311 319
13. E. Ercan, T. Ozekinci, and F. Atakul Antibacterial activity of 2% chlorhexidine gluconate and 5.25% sodium hypochlorite in infected root canal: in vivo study J Endod 30 2004 84 87
14. M.J. Jeansonne, and R.R. White A comparison of 2.0% chlorhexidine gluconate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigants J Endod 20 1994 276 278
15. J.F. Siqueira Jr., I.N. Rôas, and S.S. Paiva Bacteriologic investigation of the effects of sodium hypochlorite and chlorhexidine during the endodontic treatment of teeth with apical periodontitis Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104 2007 122 130
16. B.P. Gomes, F.C. Martinho, and M.E. Vianna Comparison of 2.5% sodium hypochlorite and 2% chlorhexidine gel on oral bacterial lipopolysaccharide reduction from primarily infected root canals J Endod 35 2009 1350 1353
17. J.F. Siqueira Jr., and I.N. Rôas Diversity of endodontic microbiota revisited J Dent Res 88 2009 969 981
18. M.E. Vianna, G. Conrads, and B.P.F.A. Gomes Identification and quantification of archaea involved in primary endodontic infections J Clin Microbiol 44 2006 1274 1282
19. J.C. Baumgartner, C.M. Watts, and T. Xia Occurrence of Candida albicans in infections of endodontic origin J Endod 26 2000 695 698
20. J.F. Siqueira Jr., T. Guimarães-Pinto, and I.N. Rôas Effects of chemomechanical preparation with 2.5% sodium hypochlorite and intracanal medication with calcium hydroxide on cultivable bacteria in infected root canals J Endod 33 2007 800 805
21. J.F. Siqueira Jr., and I.N. Rôas Uncultivated phylotypes and newly named species associated with primary and persistent endodontic infections J Clin Microbiol 43 2005 3314 3319
22. B.J. Paster, I.M. Bartoszyk, and F.E. Dewhirst Identification of oral streptococci using PCR-based, reverse-capture, checkerboard hybridization Meth Cell Sci 20 1998 223 231
23. P.W. Lepp, M.M. Brinig, and C.C. Ouverney Methanogenic archaea and human periodontal disease Proc Natl Acad Sci U S A 101 2004 6176 6181
24. D.A. Stahl, and R. Amann Development and application of nucleic acid probes in bacterial systematics E. Stackebrandt, M. Goodfellow, Nucleic acid techniques in bacterial systematics 1991 John Wiley & Sons Ltd Chichester, UK 205 248
25. K. Makimura, S.Y. Murayama, and H. Yamaguchi Detection of a wide range of medically important fungi by the polymerase chain reaction J Med Microbiol 40 1994 358 364
26. I.N. Rôas, and J.F. Siqueira Jr. Root canal microbiota of teeth with chronic apical periodontitis J Clin Microbiol 46 2008 3599 3606
27. J.F. Siqueira Jr., and I.N. Rôas The microbiota of acute apical abscesses J Dent Res 88 2009 61 65
28. M.R. Becker, B.J. Paster, and E.J. Leys Molecular analysis of bacterial species associated with childhood caries J Clin Microbiol 40 2002 1001 1009
29. I.N. Rôas, M. Hulsmann, and J.F. Siqueira Jr. Microorganisms in root canal-treated teeth from a German population J Endod 34 2008 926 931
30. A. Byström, and G. Sundqvist The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy Int Endod J 18 1985 35 40
31. G. McDonnel, and A.D. Russell Antiseptics and disinfectants: activity, action, and resistance Clin Microbiol Rev 12 1999 147 179
32. C. Yesilsoy, E. Whitaker, and D. Cleveland Antimicrobial and toxic effects of established and potential root canal irrigants J Endod 21 1995 513 515
33. G.R. Dychdala Chlorine and chlorine compounds S.S. Block, Disinfection, sterilization, and preservation 4th ed 1991 Lea & Febiger Philadelphia 133 150
34. S. Rosenthal, L. Spangberg, and K. Safavi Chlorhexidine substantivity in root canal dentin Oral Surg Oral Med Oral Pathol Oral Radiol Endod 98 2004 488 492
35. M.R. Carrilho, R.M. Carvalho, and E.N. Sousa Substantivity of chlorhexidine to human dentin Dent Mater 26 2010 779 785
36. A. Zamany, K. Safavi, and L.S. Spangberg The effect of chlorhexidine as an endodontic disinfectant Oral Surg Oral Med Oral Pathol Oral Radiol Endod 96 2003 578 581
37. S.K. Huffaker, K. Safavi, and L.S. Spangberg Influence of a passive sonic irrigation system on the elimination of bacteria from root canal systems: a clinical study J Endod 36 2010 1315 1318
38. G.B. Rogers, F.A. Stressmann, and G. Koller Assessing the diagnostic importance of nonviable bacterial cells in respiratory infections Diagn Microbiol Infect Dis 62 2008 133 141
39. I.N. Rôas, and J.F. Siqueira Jr. Identification of bacteria enduring endodontic treatment procedures by a combined reverse transcriptase-polymerase chain reaction and reverse-capture checkerboard approach J Endod 36 2010 45 52
40. S.C. McCarty, and R.M. Atlas Effect of amplicon size on PCR detection of bacteria exposed to chlorine PCR Methods Appl 3 1993 181 185