Pro-calcific responses by aortic valve interstitial cells in a novel in vitro model simulating dystrophic calcification

Italian Journal of Anatomy and Embryology

Volumn 115, Issue 1-2, 2010, Pages 135-139

  Ortolani, Fulvia ^a   Rigonat, Luca ^a   Bonetti, Antonella ^a   Contin, Magali ^a   Tubaro, Franco ^a   Rattazzi, Marcello ^b   Marchini, Maurizio ^a  

^a UNIVERSITY OF UDINE   (Italy)

^b UNIVERSITY OF PADOVA   (Italy)

Author keywords

Aortic valve stenosis; Ectopic calcification; Phthalocyanin reaction; Valve interstitial cell cultures

Indexed keywords

CALCIUM; LIPOPOLYSACCHARIDE; PHOSPHATE;

ANIMAL; AORTA VALVE; AORTA VALVE STENOSIS; ARTICLE; BIOLOGICAL MODEL; CALCINOSIS; CATTLE; CELL CULTURE; CULTURE MEDIUM; CULTURE TECHNIQUE; ENDOTHELIUM CELL; METABOLISM; METHODOLOGY; MONOCYTE; PATHOLOGY; PATHOPHYSIOLOGY; PHYSIOLOGY; SECRETION; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ANIMALS; AORTIC VALVE; AORTIC VALVE STENOSIS; CALCINOSIS; CALCIUM; CATTLE; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CULTURE MEDIA, CONDITIONED; ENDOTHELIAL CELLS; LIPOPOLYSACCHARIDES; MICROSCOPY, ELECTRON, TRANSMISSION; MODELS, BIOLOGICAL; MONOCYTES; PHOSPHATES;

EID: 78651469903     PISSN: 11226714     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. Akat K., Borggrefe M., Kaden J.J. (2009) Aortic valve calcifcation. Basic science to clinical practice. Heart 95: 616-623.
2. Ortolani F., Petrelli L., Tubaro F., Spina M., Marchini M. (2002a) Novel ultrastructural features as revealed by phthalocyanin reactions indicate cell priming for calcifca-tion in subdermally implanted aortic valves. Connect. Tiss. Res. 43: 44-55.
3. Ortolani F., Tubaro F., Petrelli L., Gandaglia A., Spina M., Marchini M. (2002b) Copper retention, calcium release and ultrastructural evidence indicate specifc cuprolinic blue uptake and peculiar modifcations in mineralizing aortic valves. Histochem. J. 34: 41-50.
4. Ortolani F., Petrelli L., Nori S.L., Spina M., Marchini M. (2003) Malachite green and phthalocyanine-silver reactions reveal acidic phospholipid involvement in calci-fcation of porcine aortic valves in rat subdermal model. Histol. Histopathol. 18: 1131-1140.
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7. Tintut Y., Patel J., Territo M., Saini T., Parhami F., Demer L.L. (2002) Monocyte/mac-rophage regulation of vascular calcifcation in vitro. Circulation 105: 650-655.