Salt split technique: A useful tool in the diagnosis of subepidermal bullous disorders

Indian Journal of Dermatology

Volumn 55, Issue 4, 2010, Pages 334-336

  De, Abhishek ^a   Rao, Raghavendra ^a   Balachandran, C ^a  

^a KASTURBA MEDICAL COLLEGE   (India)

Author keywords

Immunofluorescence; salt split technique; subepidermal bullous diseases

Indexed keywords

IMMUNOGLOBULIN G;

ADOLESCENT; ADULT; AGED; ARTICLE; BASEMENT MEMBRANE; BLISTER; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; EPIDERMOLYSIS BULLOSA ACQUISITA; FEMALE; HUMAN; IMMUNOFLUORESCENCE; MALE; PUNCH BIOPSY; SALT SPLIT TECHNIQUE; SCHOOL CHILD; SKIN BIOPSY; SUBEPIDERMAL IMMUNE BULLOUS DISEASE;

EID: 78651320567     PISSN: 00195154     EISSN: 19983611     Source Type: Journal    
DOI: 10.4103/0019-5154.74534     Document Type: Article

References (20)

1. Wong SN, Chua SH. Spectrum of subepidermal immuno-bullous disorders seen at the National skin centre, Singapore: A 2-year review. Br J Dermatol 2002;147:476-80.
2. Bernard P, Vaillant L, Bedani C. Incidence and distribution of subepidermal autoimmune bullous disease in 3 French regions. Arch Dermatol 1995;131:48-52.
3. Briggaman RA, Gammon WR, Woodley DT. Epidermolysis bullosa acquisita of the immunopathological type (dermolytic pemphigoid). J Invest Dermatol 1985;85:79s-84s.
4. Gammon WR, Briggaman RA, Woodley DT, Heald PW, Wheeler CE Jr. Epidermolysis bullosa acquisita: A pemphigoid like disease. J Am Acad Dermatol 1984;11:820-3.
5. Yaoita H, Briggaman RA, Lawley TJ, Provost TT, Katz SI. Epidermolysis bullosa acquisita: Ultrastructural and immunological studies. J Invest Dermatol 1981;76:288-92.
6. Mueller S, Klaus-Kovtum V, Stanley JR. A 230-kD basic protein is the major bullous pemphigoid antigen. J Invest Dermatol 1989;92:33-8.
7. Labib RS, Anhalt GJ, Patel HP, Mutasim DF, Diaz LA. Molecular heterogenicity of the bullous pemphigoid antigens as detected by immunoblotting. J Immunol 1986;136:1231-5.
8. Gammon WR, Kowalewski C, Chorzelski TP, Kumar V, Briggaman RA, Beutner EH. Direct immunofluorescence studies of sodium chloride- separated skin in the differential diagnosis of bullous pemphigoid and epidermolysis acquisita. J Am Acad Dermatol 1990;22:640-70.
9. Gammon WR, Briggaman RA, Inman AO, Queen LL, Wheeler CE Jr. Differentiating anti-lamina lucida and anti-sublamina densa anti-BMZ antibodies by direct immunofluorescence on 1.0 M sodium chloride- separated skin. J Invest Dermatol 1984;82:139-44.
10. Nanda A, Dvorak R, Al-Saeed K, Al-Sabah H, Alsaleh QA. Spectrum of autoimmune bullous disease in Kuwait. Int J Dermatol 2004;43:876-81.
11. Beutner EH, Jordon RE, Chorzelski TP. The immunopathology of pemphigus and bullous pemphigoid. J Invest Dermatol 1989;292:166-8.
12. Domloge-Hultsch N, Bisalbutra P, Gammon WR, Yancey KB. Direct immunofluorescence microscopy of 1 mol/L sodium chloride-treated patient skin. J Am Acad Dermatol 1991;24:946-51.
13. Satyapal S, Amladi S, Jerajani HR. Evaluation of salt split technique of immunofluorescence in bullous pemphigoid. Indian J Dermatol Venereol Leprol 2002;68:330-3.
14. Woodley DT. Immunofluorescence on salt-split skin for the diagnosis of epidermolysis bullosa acquisita. Arch Dermatol 1990;126:229-31.
15. Woodley DT, Sauder D, Talley MJ, Silver M, Grotendorst G, Qwarnstrom E. Localization of basement membrane components after dermal-epidermal junction separation. J Invest Dermatol 1983;81:149-53.
16. Logan RA, Bhogal B, Das AK, McKee PM, Black MM. Localization of bullous pemphigoid antibody: An indirect immunofluorescence study of 228 cases using a split-skin technique. Br J Dermatol 1987;117:471-8.
17. Barnadas MA, Gelpi C, Currel R, de Moragas JM, Alomar A. Repeat direct immunofluorescence (DIF) test, using 1 M NaCl treated skin, in the subepidermal autoimmune bullous diseases that contain IgG at the dermal-epidermal junction. J Cutan Pathol 1999;26:37-41.
18. Zhu XJ, Niimi Y, Bystryn JC. Epidermolysis bullosa acquisita: Incidence in patients with basement membrane antibodies. Arch Dermatol 1990;126:171-4.
19. Ghohestani RF, Nicolas JF, Rousselle P, Claudy A. Diagnostic value of indirect immunofluorescence on sodium chloride- split skin in differential diagnosis of subepidermal autoimmune bullous dermatoses. Arch Dermatol 1997;133:1102-7.
20. Pang RK, Lee YS, Ratnam KV. Floor pattern in salt split can not distinguish bullous pemphigoid from epidermolysis bullosa acquisita. Use of toad skin. Arch Dermatol 1993;129:744-6.