Implications of using the fluorescent probes, dihydrorhodamine 123 and 2′,7′-dichlorodihydrofluorescein diacetate, for the detection of UVA-induced reactive oxygen species

Free Radical Research

Volumn 45, Issue 2, 2011, Pages 115-122

  Boulton, Sarahjayne ^a   Anderson, Alasdair ^a   Swalwell, Helen ^a   Henderson, James R ^a   Manning, Philip ^a   Birch Machin, Mark A ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

DCF DA; DHR123; FACS; Fluorescence; Reactive oxygen species; UVA

Indexed keywords

DICHLORODIHYDROFLUORESCEIN DIACETATE; DIHYDRORHODAMINE 123; REACTIVE OXYGEN METABOLITE;

ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; FLUORESCENCE; HUMAN; HUMAN CELL; IN VITRO STUDY; KERATINOCYTE; RADIATION DOSE; RADIATION EXPOSURE; ULTRAVIOLET A RADIATION;

EID: 78651306084     PISSN: 10715762     EISSN: 10292470     Source Type: Journal    
DOI: 10.3109/10715762.2010.517751     Document Type: Article

References (35)

1. Cheeseman KH. Mechanisms and effects of lipid peroxida-tion. Mol Aspects Med 1993;14:191-197.
2. Cheeseman KH. Tissue injury by free radicals. Toxicol Ind Health 1993;9:39-51.
3. Halliwell B, Gutteridge J. Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy. Lancet 1984;105: 1396-1397.
4. 2 availability to ARPE-19 cultures in models of oxidative stress. Free Radic Biol Med;48:1064-1070.
5. Yuan H, Perry CN, Huang C, Iwai-Kanai E, Carreira RS, Glembotski CC, Gottlieb RA. LPS-induced autophagy is mediated by oxidative signaling in cardiomyocytes and is associated with cytoprotection. Am J Physiol: Heart Circ Physiol 2009;296:470-479.
6. Foyer CH, Noctor G. Redox homeostasis and antioxidant signaling: a metabolic interface between stress perception and physiological responses. Plant Cell 2005;17:1866-1875.
7. McNeil CJ, Manning P. Sensor-based measurements of the role and interactions of free radicals in cellular systems. Rev Mol Biotechnol 2002;82:443-455.
8. Traverso JA, Vignols F, Chueca A. Thioredoxin and redox control within the new concept of oxidative signaling. Plant Signal Behavior 2007;2:426-427.
9. Cui X-L, Ding Y, Alexander LD, Bao C, Al-Khalili OK, Simonson M, Eaton DC, Douglas JG. Oxidative signaling in renal epithelium: critical role of cytosolic phospholipase A2 and p38SAPK. Free Radic Biol Med 2006;41:213-221.
10. Aitken GR, Henderson JR, Chang SC, McNeil CJ, Birch-Machin MA. Direct monitoring of UV-induced free radical generation in HaCaT keratinocytes. Clinical Exp Der-matol 2007;32:722-727.
11. Barbacanne MA, Souchard JP, Darblade B, Iliou JP, Nepveu F, Pipy B, Bayard F, Arnal JF. Detection of superoxide anion released extracellularly by endothelial cells using cytochrome c reduction, ESR, fluorescence and lucigenin-enhanced chemi-luminescence techniques. Free Radic Biol Med 2000;29: 388-396.
12. Chang SC, Pereira-Rodrigues N, Henderson JR, Cole A, Bedioui F, McNeil CJ. An electrochemical sensor array system for the direct, simultaneous in vitro monitoring of nitric oxide and superoxide production by cultured cells. Biosens Bioelec-tron 2005;21:917-922.
13. Chang SC, Rodrigues NP, Zurgil N, Henderson JR, Bedioui F, McNeil CJ, Deutsch M. Simultaneous intra- and extracellular superoxide monitoring using an integrated optical and electrochemical sensor system. Biochem Biophys Res Comm 2005;327:979-984. (Pubitemid 40137963)
14. Henderson JR, Swalwell H, Boulton S, Manning P, McNeil CJ, Birch-Machin MA. Direct, real-time monitoring of superox-ide generation in isolated mitochondria. Free Rad Res 2009; 43:796-802.
15. Tammeveski K, Tenno TT, Mashirin AA, Hillhouse EW, Manning P, McNeil CJ. Superoxide electrode based on cova-lently immobilized cytochrome c: modelling studies. Free Radic Biol Med 1998;25:973-978.
16. Van Lente F. Free radicals. Anal Chem 1993;65:374R-377R.
17. Granzow C, Kopun M, Krober T. Riboflavin-mediated pho-tosensitization of Vinca alkaloids distorts drug sensitivity assays. Cancer Res 1995;55:4837-4843.
18. Grzelak A, Rychlik B, Bartosz G. Light-dependent generation of reactive oxygen species in cell culture media. Free Radic Biol Med 2001;30:1418-1425.
19. Long LH, Halliwell B. Artefacts in cell culture: pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in cell culture media. Biochem Bio-phys Res Comm 2009;388:700-704.
20. Soh N. Recent advances in fluorescent probes for the detec tion of reactive oxygen species. Anal Bioanal Chem 2006;386: 532-543.
21. Mahns A, Melchheier I, Suschek CV, Sies H, Klotz LO. Irradiation of cells with ultraviolet-A (320-400 nm) in the presence of cell culture medium elicits biological effects due to extracellular generation of hydrogen peroxide. Free Rad Res 2003;37:391-397.
22. Chen X, Zhong Z, Xu Z, Chen L, Wang Y. 2 ′,7 ′ -Dichlorodi-hydrofluorescein as a fluorescent probe for reactive oxygen species measurement: forty years of application and controversy. Free Rad Res 2010;44:587-604.
23. Afzal M, Matsugo S, Sasai M, Xu B, Aoyama K, Takeuchi T. Method to overcome photoreaction, a serious drawback to the use of dichlorofluorescin in evaluation of reactive oxygen species. Biochem Biophys Res Comm 2003;304:619-624.
24. Gniadecki R, Thorn T, Vicanova J, Petersen A, Wulf HC. Role of mitochondria in ultraviolet-induced oxidative stress. J Cell Biochem 2000;80:216-222.
25. Krishnan KJ, Harbottle A, Birch-Machin MA. The use of a 3895 bp mitochondrial DNA deletion as a marker for sunlight exposure in human skin. J Invest Dermatol 2004;123: 1020-1024.
26. Royall JA, Ischiropoulos H. Evaluation of 2 ′,7 ′ -dichlorofluorescin and dihydrorhodamine 123 as fluorescent probes for intracel-lular H2O2 in cultured endothelial cells. Arch Biochem Bio-phys 1993;302:348-355.
27. Cunningham ML, Krinsky NI, Giovanazzi SM, Peak MJ. Superoxide anion is generated from cellular metabolites by solar radiation and its components. J Free Radic Biol Med1985;1:381-385.
28. Wang RJ, Nixon B T. Identifi cation of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with ' daylight ' fluorescent light. In Vitro 1978;14: 715-722.
29. Valko M, Morris H, Mazur M, Telser J, McInnes EJL, Mabbs FE. High-Affi nity binding site for copper(II) in human and dog serum albumins (an EPR study). J Phys Chem B 1999; 103:5591-5597.
30. Bal W, Christodoulou J, Sadler PJ, Tucker A. Multi-metal binding site of serum albumin. J Inorg Biochem 1998;70: 33-39.
31. 3+. Eur J Biochem 1994;220:193-200.
32. Zgirski A, Frieden E. Binding of Cu(II) to non-prosthetic sites in ceruloplasmin and bovine serum albumin. J Inorg Biochem 1990;39:137-148.
33. Batandier C, Fontaine E, Keriel C, Leverve XM. Determination of mitochondrial reactive oxygen species: methodological aspects. J Cell Mol Med 2002;6:175-187.
34. Henderson JR, Fulton DA, McNeil CJ, Manning P, Henderson JR, Fulton DA, McNeil CJ, Manning P. The development and in vitro characterisation of an intracellular nanosensor responsive to reactive oxygen species. Biosens Bioelectron 2009;24:3608-3614.
35. Jee HJ, Kim HJ, Kim AJ, Bae YS, Bae SS, Yun J. UV light induces premature senescence in Akt1-null mouse embryonic fibroblasts by increasing intracellular levels of ROS. Biochem Biophys Res Comm 2009;383:358-362.