Engineering melon plants with improved fruit shelf life using the TILLING approach

PLoS ONE

Volumn 5, Issue 12, 2010, Pages

  Dahmani Mardas, Fatima ^a   Troadec, Christelle ^a   Boualem, Adnane ^a   Lévêque, Sylvie ^b   Alsadon, Abdullah A ^c   Aldoss, Abdullah A ^c   Dogimont, Catherine ^b   Bendahmane, Abdelhafid ^a,c  

^a CNRS   (France)

^b Researches Center of Avignon   (France)

^c KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

[No Author keywords available]

Indexed keywords

1 AMINOCYCLOPROPANECARBOXYLIC ACID; ETHYLENE; 1 AMINOCYCLOPROPANE 1 CARBOXYLIC ACID OXIDASE; 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID OXIDASE; ETHYLENE DERIVATIVE; OXIDOREDUCTASE;

ARTICLE; CONSERVATION GENETICS; CONTROLLED STUDY; DNA MODIFICATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; FRUIT RIPENING; GENE STRUCTURE; GENETIC ENGINEERING; MELON; MISSENSE MUTATION; MOLECULAR CLONING; MUTATION RATE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PLANT GENE; PREDICTION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; SHELF LIFE; AMINO ACID SEQUENCE; CHEMISTRY; CUCUMBER; DNA SEQUENCE; FRUIT; GENE EXPRESSION REGULATION; GENETICS; METHODOLOGY; MOLECULAR GENETICS; PHYSIOLOGY; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY; X RAY CRYSTALLOGRAPHY;

ARABIDOPSIS; CUCUMIS; LYCOPERSICON ESCULENTUM;

AMINO ACID OXIDOREDUCTASES; AMINO ACID SEQUENCE; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; CUCURBITACEAE; ETHYLENES; FRUIT; GENE EXPRESSION REGULATION, PLANT; GENETIC ENGINEERING; MOLECULAR SEQUENCE DATA; MUTATION, MISSENSE; PHENOTYPE; PROTEIN CONFORMATION; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 78651258606     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0015776     Document Type: Article

References (68)

1. Jeffrey C (1990) Systematics of the Cucurbitaceae: an overview. In: Bates DM RW, Jeffrey C, eds. Biology and utilization of the Cucurbitaceae. New-York, USA: Cornell University. pp 3-9.
2. Boualem A, Fergany M, Fernandez R, Troadec C, Martin A, et al. (2008) A conserved mutation in an ethylene biosynthesis enzyme leads to andromonoecy in melons. Science 321: 836-838.
3. Martin A, Troadec C, Boualem A, Rajab M, Fernandez R, et al. (2009) A transposon-induced epigenetic change leads to sex determination in melon. Nature 461: 1135-1138.
4. Ezura H, Owino WO (2008) Melon, an alternative model plant for elucidating fruit ripening. Plant Science 175: 121-129.
5. Pech JC, Bouzayen M, Latché A (2008) Climacteric fruit ripening: Ethylenedependent and independent regulation of ripening pathways in melon fruit Plant Science 175: 114-120.
6. Haritatos E, Keller F, Turgeon R (1996) Raffinose oligosaccharide concentrations measured in individual cell and tissue types in Cucumis melo L. leaves: implications for phloem loading. Planta 198: 614-622.
7. Gomez G, Torres H, Pallas V (2005) Identification of translocatable RNAbinding phloem proteins from melon, potential components of the long-distance RNA transport system. Plant J 41: 107-116.
8. Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Molecular Biology Reporter 9: 208-218.
9. Gonzalez-Ibeas D, Blanca J, Roig C, Gonzalez-To M, Pico B, et al. (2007) MELOGEN: an EST database for melon functional genomics. BMC Genomics 8: 306.
10. van Leeuwen H, Monfort A, Zhang HB, Puigdomenech P (2003) Identification and characterization of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana. Plant Mol Biol 51: 703-718.
11. Morales M, Orjeda G, Nieto C, van Leeuwen H, Monfort A, et al. (2005) A physical map covering the nsv locus that confers resistance to Melon necrotic spot virus in melon (Cucumis melo L.). Theor Appl Genet 111: 914-922.
12. Mascarell-Creus A, Canizares J, Vilarrasa-Blasi J, Mora-Garcia S, Blanca J, et al. (2009) An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.). BMC Genomics 10: 467.
13. Perin C, Gomez-Jimenez M, Hagen L, Dogimont C, Pech JC, et al. (2002) Molecular and genetic characterization of a non-climacteric phenotype in melon reveals two loci conferring altered ethylene response in fruit. Plant Physiol 129: 300-309.
14. Fernandez-Silva I, Eduardo I, Blanca J, Esteras C, Pico B, et al. (2008) Bin mapping of genomic and EST-derived SSRs in melon (Cucumis melo L.). Theor Appl Genet 118: 139-150.
15. Deleu W, Esteras C, Roig C, Gonzalez-To M, Fernandez-Silva I, et al. (2009) A set of EST-SNPs for map saturation and cultivar identification in melon. BMC Plant Biol 9: 90.
16. Huang S, Li R, Zhang Z, Li L, Gu X, et al. (2009) The genome of the cucumber, Cucumis sativus L. Nat Genet 41: 1275-1281.
17. Gonzalez VM, Garcia-Mas J, Arus P, Puigdomenech P (2010) Generation of a BAC-based physical map of the melon genome. BMC Genomics 11: 339.
18. Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, et al. (2003) Genomewide insertional mutagenesis of Arabidopsis thaliana. Science 301: 653-657.
19. Waterhouse PM, Graham MW, Wang MB (1998) Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc Natl Acad Sci U S A 95: 13959-13964.
20. Greene EA, Codomo CA, Taylor NE, Henikoff JG, Till BJ, et al. (2003) Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics 164: 731-740.
21. Triques K, Sturbois B, Gallais S, Dalmais M, Chauvin S, et al. (2007) Characterization of Arabidopsis thaliana mismatch specific endonucleases: application to mutation discovery by TILLING in pea. Plant J 51: 1116-1125.
22. Dalmais M, Schmidt J, Le Signor C, Moussy F, Burstin J, et al. (2008) UTILLdb, a Pisum sativum in silico forward and reverse genetics tool. Genome Biol 9: R43.
23. Le Signor C, Savois V, Aubert G, Verdier J, Nicolas M, et al. (2009) Optimizing TILLING populations for reverse genetics in Medicago truncatula. Plant Biotechnol J 7: 430-441.
24. Minoia S, Petrozza A, D'Onofrio O, Piron F, Mosca G, et al. (2010) A new mutant genetic resource for tomato crop improvement by TILLING technology. BMC Res Notes 3: 69.
25. Piron F, Nicolai M, Minoia S, Piednoir E, Moretti A, et al. (2010) An induced mutation in tomato eIF4E leads to immunity to two potyviruses. PLoS One 5: e11313.
26. Cooper JL, Till BJ, Laport RG, Darlow MC, Kleffner JM, et al. (2008) TILLING to detect induced mutations in soybean. BMC Plant Biol 8: 9.
27. Till BJ, Reynolds SH, Greene EA, Codomo CA, Enns LC, et al. (2003) Largescale discovery of induced point mutations with high-throughput TILLING. Genome Res 13: 524-530.
28. Till BJ, Reynolds SH, Weil C, Springer N, Burtner C, et al. (2004) Discovery of induced point mutations in maize genes by TILLING. BMC Plant Biol 4: 12.
29. Uauy C, Paraiso F, Colasuonno P, Tran RK, Tsai H, et al. (2009) A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat. BMC Plant Biol 9: 115.
30. Stephenson P, Baker D, Girin T, Perez A, Amoah S, et al. (2010) A rich TILLING resource for studying gene function in Brassica rapa. BMC Plant Biol 10: 62.
31. Winkler S, Schwabedissen A, Backasch D, Bokel C, Seidel C, et al. (2005) Target-selected mutant screen by TILLING in Drosophila. Genome Res 15: 718-723.
32. Moens CB, Donn TM, Wolf-Saxon ER, Ma TP (2008) Reverse genetics in zebrafish by TILLING. Brief Funct Genomic Proteomic 7: 454-459.
33. Gilchrist EJ, O'Neil NJ, Rose AM, Zetka MC, Haughn GW (2006) TILLING is an effective reverse genetics technique for Caenorhabditis elegans. BMC Genomics 7: 262.
34. Brady CJ (1987) Fruit Ripening. Annual Review of Plant Physiology 38: 155-178.
35. Brummell DA, Harpster MH (2001) Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Mol Biol 47: 311-340.
36. Adams-Phillips L, Barry C, Kannan P, Leclercq J, Bouzayen M, et al. (2004) Evidence that CTR1-mediated ethylene signal transduction in tomato is encoded by a multigene family whose members display distinct regulatory features. Plant Mol Biol 54: 387-404.
37. Giovannoni J (2001) Molecular Biology of Fruit Maturation and Ripening. Annu Rev Plant Physiol Plant Mol Biol 52: 725-749.
38. Giovannoni JJ (2004) Genetic regulation of fruit development and ripening. Plant Cell 16(Suppl) pp S170-180.
39. Seymour G, Poole M, Manning K, King GJ (2008) Genetics and epigenetics of fruit development and ripening. Curr Opin Plant Biol 11: 58-63.
40. Barry C, Giovannoni JJ (2007) Ethylene and Fruit Ripening Journal of Plant Growth Regulation 26: 143-159.
41. Giovannoni JJ (2007) Fruit ripening mutants yield insights into ripening control. Curr Opin Plant Biol 10: 283-289.
42. Vrebalov J, Ruezinsky D, Padmanabhan V, White R, Medrano D, et al. (2002) A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor (rin) locus. Science 296: 343-346.
43. Manning K, Tor M, Poole M, Hong Y, Thompson AJ, et al. (2006) A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening. Nat Genet 38: 948-952.
44. Wilkinson JQ, Lanahan MB, Yen HC, Giovannoni JJ, Klee HJ (1995) An ethylene-inducible component of signal transduction encoded by never-ripe. Science 270: 1807-1809.
45. Alexander L, Grierson D (2002) Ethylene biosynthesis and action in tomato: a model for climacteric fruit ripening. J Exp Bot 53: 2039-2055.
46. Lin Z, Zhong S, Grierson D (2009) Recent advances in ethylene research. J Exp Bot 60: 3311-3336.
47. Ayub R, Guis M, Ben Amor M, Gillot L, Roustan JP, et al. (1996) Expression of ACC oxidase antisense gene inhibits ripening of cantaloupe melon fruits. Nat Biotechnol 14: 862-866.
48. Wu JL, Wu C, Lei C, Baraoidan M, Bordeos A, et al. (2005) Chemical- and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics. Plant Mol Biol 59: 85-97.
49. Chawade A, Sikora P, Brautigam M, Larsson M, Vivekanand V, et al. (2010) Development and characterization of an oat TILLING-population and identification of mutations in lignin and beta-glucan biosynthesis genes. BMC Plant Biol 10: 86.
50. Gottwald S, Bauer P, Komatsuda T, Lundqvist U, Stein N (2009) TILLING in the two-rowed barley cultivar 'Barke' reveals preferred sites of functional diversity in the gene HvHox1. BMC Res Notes 2: 258.
51. Wang N, Wang Y, Tian F, King GJ, Zhang C, et al. (2008) A functional genomics resource for Brassica napus: development of an EMS mutagenized population and discovery of FAE1 point mutations by TILLING. New Phytol 180: 751-765.
52. Till BJ, Cooper J, Tai TH, Colowit P, Greene EA, et al. (2007) Discovery of chemically induced mutations in rice by TILLING. BMC Plant Biol 7: 19.
53. Caldwell DG, McCallum N, Shaw P, Muehlbauer GJ, Marshall DF, et al. (2004) A structured mutant population for forward and reverse genetics in Barley (Hordeum vulgare L.). Plant J 40: 143-150.
54. Silva JA, Da Costa TS, Lucchetta L, Marini LJ, Zanuzo MR, et al. (2004) Characterization of ripening behavior in transgenic melons expressing an antisense 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene from apple. Postharvest Biology and Technology 32: 263-268.
55. Nunez-Palenius HG, Cantliffe DJ, Huber DJ, Ciardi J, Klee HJ (2006) Transformation of a muskmelon 'Galia' hybrid parental line (Cucumis melo L. var. reticulatus Ser.) with an antisense ACC oxidase gene. Plant Cell Rep 25: 198-205.
56. Hamilton AJ, Lycett GW, Grierson D (1990) Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants. Nature 346: 284-287.
57. Murray AJ, Hobson GE, Schuch W, Bird CR (1993) Reduced ethylene synthesis in EFE antisense tomatoes has differential effects on fruit ripening processes. Postharvest Biology and Technology 2: 301-313.
58. Xiong AS, Yao QH, Peng RH, Li X, Han PL, et al. (2005) Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato. Plant Cell Rep 23: 639-646.
59. Ng, Henikoff (2003) SIFT: Predicting amino acid changes that affect protein function. Nucleic acids research 31: 3812-3814.
60. Zhang Z, Ren JS, Clifton IJ, Schofield CJ (2004) Crystal structure and mechanistic implications of 1-aminocyclopropane-1-carboxylic acid oxidase-the ethylene-forming enzyme. Chem Biol 11: 1383-1394.
61. Zhang Z, Ren J, Stammers DK, Baldwin JE, Harlos K, et al. (2000) Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase. Nat Struct Biol 7: 127-133.
62. Roach PL, Clifton IJ, Fulop V, Harlos K, Barton GJ, et al. (1995) Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes. Nature 375: 700-704.
63. Roach PL, Clifton IJ, Hensgens CM, Shibata N, Schofield CJ, et al. (1997) Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation. Nature 387: 827-830.
64. Valegard K, van Scheltinga AC, Lloyd MD, Hara T, Ramaswamy S, et al. (1998) Structure of a cephalosporin synthase. Nature 394: 805-809.
65. Elkins JM, Ryle MJ, Clifton IJ, Dunning Hotopp JC, Lloyd JS, et al. (2002) Xray crystal structure of Escherichia coli taurine/alpha-ketoglutarate dioxygenase complexed to ferrous iron and substrates. Biochemistry 41: 5185-5192.
66. Slade AJ, Fuerstenberg SI, Loeffler D, Steine MN, Facciotti D (2005) A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. Nat Biotechnol 23: 75-81.
67. Boualem A, Troadec C, Kovalski I, Sari MA, Perl-Treves R, et al. (2009) A conserved ethylene biosynthesis enzyme leads to andromonoecy in two Cucumis species. PLoS One 4: e6144.
68. Voss HD (1992) Relating colorimeter measurement of plant color to the Royal Horticultural Society Colour Chart. HortScience 27: 1256-1260.