Simultaneous virus-specific detection of the two cassava brown streak-associated viruses by RT-PCR reveals wide distribution in East Africa, mixed infections, and infections in Manihot glaziovii

Journal of Virological Methods

Volumn 171, Issue 2, 2011, Pages 394-400

  Mbanzibwa, D R ^a,b,c   Tian, Y P ^b   Tugume, A K ^c   Mukasa, S B ^c   Tairo, F ^a   Kyamanywa, S ^c   Kullaya, A ^a   Valkonen, J P T ^b  

^a Mikocheni Agricultural Research Institute   (Tanzania)

^b UNIVERSITY OF HELSINKI   (Finland)

^c MAKERERE UNIVERSITY   (Uganda)

Author keywords

Cassava brown streak virus; Manihot esculenta; Manihot glaziovii; Virus diagnostics; Virus reservoir

Indexed keywords

ARTICLE; CASSAVA BROWN STREAK VIRUS; CONTROLLED STUDY; GEOGRAPHIC ORIGIN; MIXED INFECTION; MOLECULAR PHYLOGENY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT LEAF; POTYVIRUS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TANZANIA; UGANDA; UGANDAN CASSAVA BROWN STREAK VIRUS; VIRAL PLANT DISEASE; VIRUS DETECTION; VIRUS ISOLATION;

AFRICA, EASTERN; CLUSTER ANALYSIS; MANIHOT; MOLECULAR SEQUENCE DATA; PHYLOGENY; PLANT DISEASES; POTYVIRIDAE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY; VIROLOGY;

CASSAVA BROWN STREAK VIRUS; IPOMOVIRUS; MANIHOT ESCULENTA; MANIHOT GLAZIOVII; POTYVIRIDAE;

EID: 78651254173     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.09.024     Document Type: Article

References (28)

1. Abarshi M.M., Mohammed I.U., Wasswa P., Hillocks R.J., Holt J., Legg J.P., Seal S.E., Maruthi M.N. Optimization of diagnostic RT-PCR protocols and sampling procedures for the reliable and cost-effective detection of Cassava brown streak virus. J. Virol. Methods 2010, 163:353-359.
2. Alicai T., Omongo C.A., Maruthi M.N., Hillocks R.J., Baguma Y., Kawuki R., Bua A., Otim-Nape G.W., Colvin J. Re-emergence of cassava brown streak disease in Uganda. Plant Dis. 2007, 91:24-29.
3. Allen G.C., Flores-Vergara M.A., Krasnyanski S., Kumar S., Thompson W.F. A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nat. Protocols 2006, 1:2320-2325.
4. Bock K.R. Studies on cassava brown streak virus disease in Kenya. Trop. Sci. 1994, 34:134-145.
5. Hall T.A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 1999, 41:95-98.
6. Hillocks R.J., Jennings D.L. Cassava brown streak disease: a review of present knowledge and research needs. Int. J. Pest Manag. 2003, 49:225-234.
7. Hillocks R.J., Raya M.D., Mtunda K., Kiozia H. Effects of brown streak virus disease on yield and quality of cassava in Tanzania. J. Phytopathol. 2001, 149:389-394.
8. Hillocks R.J., Raya M.D., Thresh J.M. The association between root necrosis and above-ground symptoms of brown streak virus infection of cassava in southern Tanzania. Int. J. Pest Manag. 1996, 42:285-289.
9. Hillocks R.J., Thresh J.M. Cassava mosaic and cassava brown streak virus diseases in Africa: a comparative guide to symptoms and aetiologies. Roots 2000, 7:1-8.
10. Hillocks R.J., Thresh J.M., Tomas J., Botao M., Macia R., Zavier R. Cassava brown streak disease in northern Mozambique. Int. J. Pest Manag. 2002, 48:178-191.
11. ICRAF Manihot glaziovii. AgroForestryTree Database 2010, World Agroforestry Center.
12. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 1980, 16:111-120.
13. Lister R.M. Mechanical transmission of cassava brown streak virus. Nature 1959, 183:1588-1589.
14. Lyimo M., Temu R.P.C., Mugula J.K. Identification and nutrient composition of indigenous vegetables of Tanzania. Plant Foods Hum. Nutr. 2003, 58:85-92.
15. Maruthi M.N., Hillocks R.J., Mtunda K., Raya M.D., Muhanna M., Kiozia H., Rekha A.R., Colvin J., Thresh J.M. Transmission of Cassava brown streak virus by Bemisia tabaci (Gennadius). J. Phytopathol. 2005, 153:307-312.
16. Mbanzibwa D.R., Tian Y.P., Mukasa S.B., Valkonen J.P.T. Cassava brown streak virus (Potyviridae) encodes a putative Maf/HAM1 pyrophosphatase implicated in reduction of mutations and a P1 proteinase that suppresses RNA silencing but contains no HC-Pro. J. Virol. 2009, 83:6934-6940.
17. Mbanzibwa D.R., Tian Y.P., Tugume A.K., Mukasa S.B., Tairo F., Kyamanywa S., Kullaya A., Valkonen J.P.T. Genetically distinct strains of Cassava brown streak virus in the Lake Victoria basin and the Indian Ocean coastal area of East Africa. Arch. Virol. 2009, 154:353-359.
18. Monger W.A., Alicai T., Ndunguru J., Kinyua Z.M., Potts M., Reeder R.H., Miano D.W., Adams I.P., Boonham N., Glover R.H., Smith J. The complete genome sequence of the Tanzanian strain of Cassava brown streak virus and comparison with the Ugandan strain sequence. Arch. Virol. 2010, 155:429-433.
19. Monger W.A., Seal S., Cotton S., Foster G.D. Identification of different isolates of Cassava brown streak virus and development of a diagnostic test. Plant Pathol. 2001, 50:768-775.
20. Monger W.A., Seal S., Isaac A.M., Foster G.D. Molecular characterization of the Cassava brown streak virus coat protein. Plant Pathol. 2001, 50:527-534.
21. Mware B., Olubayo F., Narla R., Songa J., Amata R., Kyamanywa S., Ateka E.M. First record of spiraling whitefly in coastal Kenya: emergence, host range, distribution and association with cassava brown streak virus disease. Int. J. Agric. Biol. 2010, 12:411-415.
22. Mware B.O., Ateka E.M., Songa J.M., Narla R.D., Olubayo F., Amata R. Transmission and distribution of cassava brown streak virus disease in cassava growing areas of Kenya. J. Appl. Biosci. 2009, 16:864-870.
23. Nichols R.F.W. The brown streak disease of cassava: distribution climatic effects and diagnostic symptoms. East Afr. Agric. J. 1950, 15:154-160.
24. Pallangyo B., Hanna R., Toko M., Gnanvossou D., Mgoo V., Otema M., Onzo A., Hountondji F., Nsami E., Mfugale O. Biological control of cassava green mite in Tanzania. Proceedings of the 9th ISTRC-AB Symposium 2007, 597-605. International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria.
25. Sserubombwe W.S., Briddon R.W., Baguma Y.K., Ssemakula G.N., Bull S.E., Bua A., Alicai T., Omongo C., Otim-Nape G.W., Stanley J. Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Müll Arg.) in Uganda. J. Gen. Virol. 2008, 89:1759-1769.
26. Storey H.H. Virus diseases of East African plants: VI-A progress report on studies of the disease of cassava. East Afr. Agric. J. 1936, 2:34-39.
27. Tamura K., Dudley J., Nei M., Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007, 24:1596-1599.
28. Winter S., Koerbler M., Stein B., Pietruszka A., Paape M., Butgereitt A. The analysis of cassava brown streak viruses reveals the presence of a distinct virus species causing cassava brown streak disease in East Africa. J. Gen. Virol. 2010, 91:1365-1372.