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8
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18944381675
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R.L. Adams, I.P. Adams, S.W. Lindow, W. Zhong, and S.L. Atkin Br. J. Cancer 92 2005 1493
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(2005)
Br. J. Cancer
, vol.92
, pp. 1493
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Adams, R.L.1
Adams, I.P.2
Lindow, S.W.3
Zhong, W.4
Atkin, S.L.5
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9
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78651251417
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note
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3 cells) were seeded on 96-well microtitre plates in F12K medium with 10% FBS and incubated overnight. The cell culture medium was replaced by the different dose of a solution of 1 and the other compounds, and then the cells were cultured for another 72 h. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reagent was added to the cell supernatant for a final concentration of 0.5 mg/ml of MTT. After 3 h the cell culture medium was removed. Formazan crystals in adherent cells were dissolved in 200 μl DMSO and the absorbance of the formazan solution was measured. The compound was tested in triplicate at each concentration and the experiments were repeated three times.
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10
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77951971475
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2. Tube formation was observed and photographed under a microscope. At least five visual fields were counted and the average number of tubes per field was calculated using light microscope. The experiments were repeated three times
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2. Tube formation was observed and photographed under a microscope. At least five visual fields were counted and the average number of tubes per field was calculated using light microscope. The experiments were repeated three times.
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(2010)
Chem. Biol. Drug Des.
, vol.75
, pp. 570
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Zhao, Y.1
Zhou, Y.2
O'Boyle, K.M.3
Murphy, P.V.4
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11
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78651239158
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note
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See Ref. 8. Cells were seeded on six well plates in F12K medium with 10% FBS overnight. Then 1 (20 μM) was added to the cells, which were then cultured for another 24 h. Cells were collected with trypsin-EDTA and washed three times with PBS. The cells were re-suspended and fixed for at least 2 h at -20 °C with 70% ethanol. After washing twice with PBS, cells were incubated at 25 °C with 200 μg/ml RNase A for 30 min. The resulting cells were incubated with 50 μg/ml propidium iodide for 30 min at 4 °C. The treated cells were subjected to flow cytometry and the percentage of cells at each phase of the cell cycle was analyzed. Compound 1 was tested in triplicate and the experiment was repeated three times.
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12
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78449286746
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Jun 30. [Epub ahead of print] Briefly, after treatment with 1 (0, 10 and 20 μM for 24 and 48 h), HUVEC cells were collected and washed with DPBS twice, and subjected to annexin V and propidium iodide staining using Annexin-V FITC apoptosis kit following the step-by-step protocol provided by the manufacturer. After staining, flow cytometry was performed for the quantification of apoptotic cells. Compound 1 was tested in triplicate and the experiment was repeated three times in each case
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Zhao, Y.; Yang, G.; Ren, D.; Zhang, X.; Yin, Q.; Sun, X. Mol. Biol. Rep. 2010 Jun 30. [Epub ahead of print] Briefly, after treatment with 1 (0, 10 and 20 μM for 24 and 48 h), HUVEC cells were collected and washed with DPBS twice, and subjected to annexin V and propidium iodide staining using Annexin-V FITC apoptosis kit following the step-by-step protocol provided by the manufacturer. After staining, flow cytometry was performed for the quantification of apoptotic cells. Compound 1 was tested in triplicate and the experiment was repeated three times in each case.
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(2010)
Mol. Biol. Rep.
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Zhao, Y.1
Yang, G.2
Ren, D.3
Zhang, X.4
Yin, Q.5
Sun, X.6
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15
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78651236491
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note
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4 cells) were seeded on 96-well microtitre plates in F12K with 10% FBS overnight. The confluent monolayers were wounded with a 200 μl plastic pipette tip. After washing, the chamber slide was incubated at 37 °C in 10% FBS F12K media in the presence or absence of compound. Migration of cells into wounded areas were photographed at 0 and 24 h.
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16
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23744513065
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D. Palm, K. Lang, B. Brandt, K.S. Zaenker, and F. Entschladen Semin. Cancer Biol. 15 2005 396
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(2005)
Semin. Cancer Biol.
, vol.15
, pp. 396
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Palm, D.1
Lang, K.2
Brandt, B.3
Zaenker, K.S.4
Entschladen, F.5
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18
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78651234332
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note
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10. Cells were seeded to the glass coverslips in cell culture dishes (3.5 cm) overnight. Compound 1 was then added and cells were cultured for 24 h. After fixing with 4% paraformaldehyde, cells were treated with 0.1% Triton X-100 and blocked with 1% BSA. Cells were incubated with TRITC-conjugated phalloidin for 60 min and examined under a confocal laser scanning microscope (100×, oil). The experiments were repeated at three times in each case.
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19
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33750709250
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For recent reviews on carbohydrate based peptidomimetic development see: P.V. Murphy, and J.L. Dunne Curr. Org. Synth. 3 2006 403
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(2006)
Curr. Org. Synth.
, vol.3
, pp. 403
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Murphy, P.V.1
Dunne, J.L.2
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