Characterization of the prime and non-prime active site specificities of proteases by proteome-derived peptide libraries and tandem mass spectrometry

Nature Protocols

Volumn 6, Issue 1, 2011, Pages 111-120

  Schilling, Oliver ^a   Huesgen, Pitter F ^b   Barré, Olivier ^b   Auf Dem Keller, Ulrich ^c   Overall, Christopher M ^b  

^a UNIVERSITY OF FREIBURG   (Germany)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINASE; PROTEOME; TRYPSIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOINFORMATICS; BIOTINYLATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME INACTIVATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; INCUBATION TIME; LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEOMICS; TANDEM MASS SPECTROMETRY; VALIDATION PROCESS;

CATALYTIC DOMAIN; CHROMATOGRAPHY, LIQUID; ESCHERICHIA COLI; INTERNET; PEPTIDE HYDROLASES; PEPTIDE LIBRARY; PROTEOME; SEQUENCE ANALYSIS, PROTEIN; SOFTWARE; SUBSTRATE SPECIFICITY; TANDEM MASS SPECTROMETRY;

ESCHERICHIA COLI;

EID: 78651069641     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.178     Document Type: Article

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