Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures

Journal of Bioscience and Bioengineering

Volumn 111, Issue 1, 2011, Pages 22-25

  Ujita, Minoru ^a   Yamanaka, Mayumi ^a   Maeno, Yumiko ^a   Yoshida, Koki ^a   Ohshio, Wakana ^a   Ueno, Yoshinori ^b   Banno, Yutaka ^b   Fujii, Hiroshi ^b   Okumura, Hiroki ^a  

^a MEIJO UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

Baculovirus silkworm expression system; Enzymatic activity; Hexahistidine tag; Oligosaccharide chains; Silkworm trehalase

Indexed keywords

BACULOVIRUS; ENZYMATIC ACTIVITIES; EXPRESSION SYSTEM; FUNCTIONAL EXPRESSION; FUSION PROTEINS; GLYCAN STRUCTURES; HEXAHISTIDINE TAG; HIGH ACTIVITY; IN-VITRO; N-TERMINALS; TREHALASE;

OLIGOSACCHARIDES;

ANIMALS;

GLYCAN; HYBRID PROTEIN; RECOMBINANT ENZYME; TREHALASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; GENE EXPRESSION; IN VITRO STUDY; INSECT; NONHUMAN; PROTEIN FUNCTION; SILKWORM;

ANIMALS; BACULOVIRIDAE; BOMBYX; CLONING, MOLECULAR; GENE EXPRESSION; GENETIC VECTORS; HISTIDINE; OLIGOPEPTIDES; RECOMBINANT FUSION PROTEINS; TREHALASE;

BOMBYX MORI; HEXAPODA;

EID: 78650990331     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2010.08.020     Document Type: Article

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