Measurement and analysis of equilibrium binding titrations: A beginner's guide

Methods in Enzymology

Volumn 488, Issue C, 2011, Pages 1-16

  Beckett, Dorothy ^a  

^a UNIVERSITY OF MARYLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; LIGAND; PROTEIN; RIBOSOME RNA;

ANISOTROPY; ARTICLE; BINDING KINETICS; CATALYSIS; CHEMICAL REACTION; DATA ANALYSIS; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; EQUILIBRIUM CONSTANT; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENCE SPECTROSCOPY; GEL MOBILITY SHIFT ASSAY; ISOTHERMAL TITRATION CALORIMETRY; LIGAND BINDING; MEDICAL RECORD; NONHUMAN; PLOTS AND CURVES; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN STRUCTURE, FUNCTION AND VARIABILITY; PYROCOCCUS HORIKOSHII; SIGNAL TRANSDUCTION; SPECTROSCOPY; STOICHIOMETRY; SURFACE PLASMON RESONANCE; TITRIMETRY;

EID: 78650949718     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-381268-1.00001-X     Document Type: Chapter

References (8)

1. G.G. Hammes Thermodynamics and Kinetics for the Biological Sciences. 2000 Wiley New York, NY
2. L.M. Hellman, and M.G. Fried Electrophoretic mobility shift assay (EMSA) for detecting proteinnucleic acid interactions Nat. Protoc. 2 8 2007 1849 1861
3. M.L. Johnson Why, when, and how biochemists should use least squares Anal. Biochem. 206 2 1992 215 225
4. J.R. Lakowicz Principles of Fluorescence Spectroscopy. 1983 Plenum Press New York and London
5. H.J. Motulsky, and L.A. Ransnas Fitting curves to data using nonlinear regression: A practical and nonmathematical review FASEB J. 1 5 1987 365 374
6. K.E. van Holde, W.C. Johnson, and P.S. Ho Principle of Physical Biochemistry. 2006 Pearson/Prentice Hall Upper Saddle River, NJ
7. G. Weber Protein Interactions. 1992 Chapman and Hall New York, NY
8. J. Wyman, and S.J. Gill Binding and Linkage. 1990 University Science Books Mill Valley, CA