Isolation and characterization of mesenchymal stromal cells from human degenerated nucleus pulposus: Comparison with bone marrow mesenchymal stromal cells from the same subjects

Spine

Volumn 35, Issue 26, 2010, Pages 2259-2265

  Blanco, Juan F ^a,b   Graciani, Ignacio F ^a,b   Sanchez Guijo, Fermin M ^a,b,c   Muntión, Sandra ^a,b   Hernandez Campo, Pilar ^a,b   Santamaria, Carlos ^a,b   Carrancio, Soraya ^a,b   Barbado, Maria Victoria ^a,d   Cruz, Graciela ^a,b   Gutierrez Cosío, Silvia ^b,c   Herrero, Carmen ^b,c   San Miguel, Jesus F ^a,b,c   Briñon, Jesus G ^b,c   Del Cañizo, Maria Consuelo ^a,b,c  

^a HOSPITAL UNIVERSITARIO DE SALAMANCA   (Spain)

^b ISCIII)   (Spain)

^c CSIC   (Spain)

^d UNIVERSITY OF SALAMANCA   (Spain)

Author keywords

cell therapy; degenerative disc disease; intervertebral disc; mesenchymal stromal cells

Indexed keywords

ADIPOCYTE; ADULT; AGED; ARTICLE; CELL DIFFERENTIATION; CELL EXPANSION; CELL ISOLATION; CELL STRUCTURE; CELL THERAPY; CLINICAL ARTICLE; CONTROLLED STUDY; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; INTERVERTEBRAL DISK DEGENERATION; INTERVERTEBRAL DISKECTOMY; MALE; MESENCHYME CELL; MOLECULAR BIOLOGY; NUCLEUS PULPOSUS; PRIORITY JOURNAL; STROMA CELL;

ADIPOCYTES; ADULT; AGED; BONE MARROW CELLS; CELL DIFFERENTIATION; CELLS, CULTURED; DISKECTOMY; FEMALE; HUMANS; ILIUM; IMMUNOPHENOTYPING; INTERVERTEBRAL DISK; INTERVERTEBRAL DISK DEGENERATION; LUMBAR VERTEBRAE; MALE; MESENCHYMAL STEM CELLS; MIDDLE AGED; TISSUE THERAPY;

EID: 78650936677     PISSN: 03622436     EISSN: 15281159     Source Type: Journal    
DOI: 10.1097/BRS.0b013e3181cb8828     Document Type: Article

References (26)

1. Waddell G. Low back disability: a syndrome of Western civilization. Neurosurg Clin N Am 1991;2:719-38.
2. Katz JN. Lumbar disc disorders and low-back pain: socioeconomic factors and consequences. J Bone Joint Surg Am 2006;88(suppl 2):21-4.
3. Waddell G. Low back pain: a twentieth century health care enigma. Spine 1996;21:2820-5.
4. Grunhagen T, Wilde G, Soukane DM, et al. Nutrient supply and intervertebral disc metabolism. J Bone Joint Surg Am 2006;88(suppl 2):30-5.
5. Anderson DG, Albert TJ, Fraser JK, et al. Cellular therapy for disc degeneration. Spine 2005;30:S14-9.
6. Leung VY, Chan D, Cheung KM. Regeneration of intervertebral disc by mesenchymal stem cells: potentials, limitations, and future direction. Eur Spine J 2006;15(suppl 3):S406-13.
7. Loeffler M, Roeder I. Tissue stem cells: definition, plasticity, heterogeneity, self-organization and models - a conceptual approach. Cells Tissues Organs 2002;171:8-26.
8. Appelbaum FR. Hematopoietic-cell transplantation at 50. N Engl J Med 2007;357:1472-5.
9. Dazzi F, Ramasamy R, Glennie S, et al. The role of mesenchymal stem cells in haemopoiesis. Blood Rev 2006;20:161-71.
10. Krampera M, Pizzolo G, Aprili G, et al. Mesenchymal stem cells for bone, cartilage, tendon and skeletal muscle repair. Bone 2006;39:678-83.
11. Jethva R, Otsuru S, Dominici M, et al. Cell therapy for disorders of bone. Cytotherapy 2009;11:3-17.
12. Sakai D, Mochida J, Iwashina T, et al. Differentiation of mesenchymal stem cells transplanted to a rabbit degenerative disc model: potential and limitations for stem cell therapy in disc regeneration. Spine 2005;30:2379-87.
13. Zhang YG, Guo X, Xu P, et al. Bone mesenchymal stem cells transplanted into rabbit intervertebral discs can increase proteoglycans. Clin Orthop Relat Res 2005:219-26.
14. Sakai D, Mochida J, Iwashina T, et al. Regenerative effects of transplanting mesenchymal stem cells embedded in atelocollagen to the degenerated intervertebral disc. Biomaterials 2006;27:335-45.
15. Risbud MV, Guttapalli A, Tsai TT, et al. Evidence for skeletal progenitor cells in the degenerate human intervertebral disc. Spine 2007;32:2537-44.
16. Dominici M, Le BK, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006;8:315-7.
17. Griffith JF, Wang YX, Antonio GE, et al. Modified Pfirrmann grading system for lumbar intervertebral disc degeneration. Spine 2007;32:E708-12.
18. Carrancio S, Lopez-Holgado N, Sanchez-Guijo FM, et al. Optimization of mesenchymal stem cell expansion procedures by cell separation and culture conditions modification. Exp Hematol 2008;36:1014-21.
19. Sanchez-Guijo FM, Blanco JF, Cruz G, et al. Multiparametric comparison of mesenchymal stromal cells obtained from trabecular bone by using a novel isolation method with those obtained by iliac crest aspiration from the same subjects. Cell Tissue Res 2009;336:501-7.
20. Fujita N, Miyamoto T, Imai J, et al. CD24 is expressed specifically in the nucleus pulposus of intervertebral discs. Biochem Biophys Res Commun 2005;338:1890-6.
21. Caplan AI. All MSCs are pericytes? Cell Stem Cell 2008;3:229-30.
22. Sacchetti B, Funari A, Michienzi S, et al. Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment. Cell 2007;131:324-36.
23. Guilak F, Cohen DM, Estes BT, et al. Control of stem cell fate by physical interactions with the extracellular matrix. Cell Stem Cell 2009;5:17-26.
24. Rastogi A, Thakore P, Leung A, et al. Environmental regulation of notochordal gene expression in nucleus pulposus cells. J Cell Physiol 2009;220: 698-705.
25. Steck E, Bertram H, Abel R, et al. Induction of intervertebral disc-like cells from adult mesenchymal stem cells. Stem Cells 2005;23:403-11.
26. Vadala G, Studer RK, Sowa G, et al. Coculture of bone marrow mesenchymal stem cells and nucleus pulposus cells modulate gene expression profile without cell fusion. Spine (Phila Pa 1976) 2008;33:870-6.