Isolation of proteinase K-sensitive prions using pronase E and phosphotungstic acid

PLoS ONE

Volumn 5, Issue 12, 2010, Pages

  D'Castro, Laura ^a   Wenborn, Adam ^a   Gros, Nathalie ^a   Joiner, Susan ^a   Cronier, Sabrina ^a,b   Collinge, John ^a   Wadsworth, Jonathan D F ^a  

^a NATIONAL HOSPITAL FOR NEUROLOGY AND NEUROSURGERY   (United Kingdom)

^b INRA Institut National de la Recherche Agronomique   (France)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHOTUNGSTIC ACID; PRION PROTEIN; PRONASE; PRONASE E; PROTEINASE K; THERMOLYSIN; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BRAIN HOMOGENATE; CONTROLLED STUDY; EXPERIMENTAL INFECTION; MOUSE; NONHUMAN; PRECIPITATION; PROTEIN CONTENT; PROTEIN DEGRADATION; PROTEIN DEPLETION; PROTEIN ISOLATION; TEMPERATURE SENSITIVITY; ANIMAL; BRAIN; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; METABOLISM; METHODOLOGY; PRION; PRION DISEASE; SCRAPIE; SILVER STAINING;

ANIMALIA;

ANIMALS; BRAIN; ENDOPEPTIDASE K; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; MICE; PHOSPHOTUNGSTIC ACID; PRION DISEASES; PRIONS; PRONASE; PRPSC PROTEINS; SCRAPIE; SILVER STAINING;

EID: 78650928306     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0015679     Document Type: Article

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