Safety assessment of nonbrowning potatoes: Opening the discussion about the relevance of substantial equivalence on next generation biotech crops

Plant Biotechnology Journal

Volumn 9, Issue 2, 2011, Pages 136-150

  Llorente, Briardo ^a   Alonso, Guillermo D ^a   Bravo Almonacid, Fernando ^a   Rodríguez, Vanina ^a   López, Mariana G ^b,c   Carrari, Fernando ^b,c   Torres, Héctor N ^a   Flawiá, Mirtha M ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b Instituto de Biotecnología   (Argentina)

^c MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

Author keywords

Biotech crop; GMO safety assessment; Polyphenol oxidase; Potato quality improvement; Solanum tuberosum; Tuber browning

Indexed keywords

ALLERGEN; CATECHOL OXIDASE;

ANIMAL; ARTICLE; BAGG ALBINO MOUSE; BIOLOGY; FEMALE; FOOD SAFETY; GENE SILENCING; GENETIC ENGINEERING; GENETICS; MOUSE; PHOTOSYNTHESIS; POTATO;

ALLERGENS; ANIMALS; CATECHOL OXIDASE; COMPUTATIONAL BIOLOGY; FEMALE; FOOD SAFETY; GENE SILENCING; GENETIC ENGINEERING; MICE; MICE, INBRED BALB C; PHOTOSYNTHESIS; SOLANUM TUBEROSUM;

SOLANUM TUBEROSUM; TUBER (TRUFFLE);

EID: 78650865671     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/j.1467-7652.2010.00534.x     Document Type: Article

References (55)

1. Aalberse, R.C. (2000) Structural biology of allergens. J. Allergy Clin. Immunol. 106, 228-238.
2. Bachem, C.W.B., Speckmann, G.-J., Van der Linde, P.C.G., Verheggen, F.T.M., Hunt, M.D., Steffens, J.C. and Zabeau, M. (1994) Antisense expression of polyphenol oxidase genes inhibits enzymatic browning in potato tubers. Biotechnology, 12, 1101-1105.
3. Baker, J.M., Hawkins, N.D., Ward, J.L., Lovegrove, A., Napier, J.A., Shewry, P.R. and Beale, M.H. (2006) A metabolomic study of substantial equivalence of field-grown genetically modified wheat. Plant Biotechnol. J. 4, 381-392.
4. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
5. Catchpole, G.S., Beckmann, M., Enot, D.P., Mondhe, M., Zywicki, B., Taylor, J., Hardy, N., Smith, A., King, R.D., Kell, D.B., Fiehn, O. and Draper, J. (2005) Hierarchical metabolomics demonstrates substantial compositional similarity between genetically modified and conventional potato crops. Proc. Natl Acad. Sci. U.S.A. 102, 14458-14462.
6. Cellini, F., Chesson, I., Colquhoun, I.J., Constable, A., Davies, H.V., Engel, K.H., Gatehouse, A.M.R., Karenlampi, S.O., Kok, E.J., Leguay, J.-J., Lehesranta, S.J., Noteborn, H.P.J.M., Pedersen, J. and Smith, M. (2004) Unintended effects and their detection in genetically modified crops. Food Chem. Toxicol. 42, 1089-1125.
7. Codex Alimentarius Commission (2003) Guidelines for the conduct of food safety assessment of foods derived from recombinant-DNA plants, Annex on the assessment of possible allergenicity, (Programme JFWFS ed) pp. 47-60, Rome.
8. Coetzer, C., Corsini, D., Love, S., Pavek, J. and Tumer, N. (2001) Control of enzymatic browning in potato (Solanum tuberosum L.) by sense and antisense RNA from tomato polyphenol oxidase. J. Agric. Food. Chem. 49, 652-657.
9. Colquhoun, I.J., Le Gall, G., Elliott, K.A., Mellon, F.A. and Michael, A.J. (2006) Shall I compare thee to a GM potato? Trends Genet. 22, 525-528.
10. Corsini, D.L., Pavek, J.J. and Dean, B. (1992) Differences in free and protein-bound tyrosine among potato genotypes and the relationship to internal blackspot resistance. Am. J. Potato Res. 69, 423-435.
11. Edwards, K., Johnstone, C. and Thompson, C. (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res. 19, 1349.
12. EFSA GMO Panel Working Group on Animal Feeding Trials (2008) Safety and nutritional assessment of GM plants and derived food and feed: the role of animal feeding trials. Food Chem. Toxicol. 46(Suppl 1), S2-S70.
13. Fernie, A.R., Roscher, A., Ratcliffe, R.G. and Kruger, N.J. (2001) Fructose 2, 6-bisphosphate activates pyrophosphate: fructose-6-phosphate 1-phosphotransferase and increases triose phosphate to hexose phosphate cycling in heterotrophic cells. Planta, 212, 250-263.
14. Fernie, A.R., Trethewey, R.N., Krotzky, A.J. and Willmitzer, L. (2004) Metabolite profiling: from diagnostics to system biology. Nat. Rev. Mol. Cell Biol. 5, 763-769.
15. Friedman, M. (1996) Food browning and its prevention: an overview. J. Agric. Food. Chem. 44, 631-653.
16. Friedman, M. (1997) Chemistry, biochemistry, and dietary role of potato polyphenols. A review. J. Agric. Food. Chem. 45, 1523-1540.
17. Gomes, N.P., Bjerke, G., Llorente, B., Szostek, S.A., Emerson, B.M. and Espinosa, J.M. (2006) Gene-specific requirement for P-TEFb activity and RNA polymerase II phosphorylation within the p53 transcriptional program. Genes Dev. 20, 601-612.
18. Goodman, R.E. (2006) Practical and predictive bioinformatics methods for the identification of potentially cross-reactive protein matches. Mol. Nutr. Food Res. 50, 655-660.
19. Goodman, R.E. and Wise, J. (2006) Bioinformatic Analysis of Proteins in Golden Rice 2 to Assess Potential Allergenic Cross-reactivity, pp. 1-24. Lincoln: Food Allergy Research and Resource Programe, Food Science and Technology, University of Nebraska-Lincoln.
20. Goodman, R.E., Vieths, S., Sampson, H.A., Hill, D., Ebisawa, M., Taylor, S.L. and Van Ree, R. (2008) Allergenicity assessment of genetically modified crops-what makes sense? Nat. Biotechnol. 26, 73-81.
21. Graff, G.D., Zilberman, D. and Bennett, A.B. (2009) The contraction of agbiotech product quality innovation. Nat. Biotechnol. 27, 702-704.
22. Graham, M., Ko, L., Hardy, V., Robinson, S., Sawyer, B., O'Hare, T., Jobin, M., Dahler, J., Underhill, S. and Smith, M. (2000) The development of blackheart resistant pineapples through genetic engineering. Acta Hortic. 529, 133-138.
23. Haas, B.J., Kamoun, S., Zody, M.C., Jiang, R.H., Handsaker, R.E., Cano, L.M., Grabherr, M., Kodira, C.D., Raffaele, S., Torto-Alalibo, T., Bozkurt, T.O., Ah-Fong, A.M., Alvarado, L., Anderson, V.L., Armstrong, M.R., Avrova, A., Baxter, L., Beynon, J., Boevink, P.C., Bollmann, S.R., Bos, J.I., Bulone, V., Cai, G., Cakir, C., Carrington, J.C., Chawner, M., Conti, L., Costanzo, S., Ewan, R., Fahlgren, N., Fischbach, M.A., Fugelstad, J., Gilroy, E.M., Gnerre, S., Green, P.J., Grenville-Briggs, L.J., Griffith, J., Grunwald, N.J., Horn, K., Horner, N.R., Hu, C.H., Huitema, E., Jeong, D.H., Jones, A.M., Jones, J.D., Jones, R.W., Karlsson, E.K., Kunjeti, S.G., Lamour, K., Liu, Z., Ma, L., Maclean, D., Chibucos, M.C., McDonald, H., McWalters, J., Meijer, H.J., Morgan, W., Morris, P.F., Munro, C.A., O'Neill, K., Ospina-Giraldo, M., Pinzon, A., Pritchard, L., Ramsahoye, B., Ren, Q., Restrepo, S., Roy, S., Sadanandom, A., Savidor, A., Schornack, S., Schwartz, D.C., Schumann, U.D., Schwessinger, B., Seyer, L., Sharpe, T., Silvar, C., Song, J., Studholme, D.J., Sykes, S., Thines, M., van de Vondervoort, P.J., Phuntumart, V., Wawra, S., Weide, R., Win, J., Young, C., Zhou, S., Fry, W., Meyers, B.C., van West, P., Ristaino, J., Govers, F., Birch, P.R., Whisson, S.C., Judelson, H.S. and Nusbaum, C. (2009) Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans. Nature, 461, 393-398.
24. James, C. (2008) Global Status of Commercialized Biotech/GM Crops:2008. Ithaca, NY: International Service for the Acquisition of Agric-biotech Applications.
25. Juberg, D.R., Herman, R.A., Thomas, J., Brooks, K.J. and Delaney, B. (2009) Acute and repeated dose (28 day) mouse oral toxicology studies with Cry34Ab1 and Cry35Ab1 Bt proteins used in coleopteran resistant DAS-59122-7 corn. Regul. Toxicol. Pharmacol. 54, 154-163.
26. Kok, E.J. and Kuiper, H.A. (2003) Comparative safety assessment for biotech crops. Trends Biotechnol. 21, 439-444.
27. Kuiper, H.A., Kok, E.J. and Engel, K.H. (2003) Exploitation of molecular profiling techniques for GM food safety assessment. Curr. Opin. Biotechnol. 14, 238-243.
28. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
29. Lehrer, S.B. and Bannon, G.A. (2005) Risks of allergic reactions to biotech proteins in foods: perception and reality. Allergy, 60, 559-564.
30. Lisec, J., Schauer, N., Kopka, J., Willmitzer, L. and Fernie, A.R. (2006) Gas chromatography mass spectrometry-based metabolite profiling in plants. Nat. Protoc. 1, 387-396.
31. Lu, Y., Xu, W., Kang, A., Luo, Y., Guo, F., Yang, R., Zhang, J. and Huang, K. (2007) Prokaryotic expression and allergenicity assessment of hygromycin B phosphotransferase protein derived from genetically modified plants. J. Food Sci. 72, 228-232.
32. Luedemann, A., Strassburg, K., Erban, A. and Kopka, J. (2008) TagFinder for the quantitative analysis of gas chromatography-mass spectrometry (GC-MS)-based metabolite profiling experiments. Bioinformatics, 24, 732-737.
33. Marqués, L., Fleuriet, A., Cleyet-Marel, J. and Macheix, J.J. (1994) Purification of an apple polyphenoloxidase isoform resistant to SDS-proteinase K digestion. Phytochemistry, 36, 1117-1121.
34. Martinez, M.V. and Whitaker, J.R. (1995) The biochemistry and control of enzymatic browning. Trends Food Sci. Technol. 6, 195-200.
35. Mathesius, C.A., Barnett, J.F.J., Cressman, R.F., Ding, J., Carpenter, C., Ladics, G.S., Schmidt, J., Layton, R.J., Zhang, J.X., Appenzeller, L.M., Carlson, G., Ballou, S. and Delaney, B. (2009) Safety assessment of a modified acetolactate synthase protein (GM-HRA) used as a selectable marker in genetically modified soybeans. Regul. Toxicol. Pharmacol. 55, 309-320.
36. Mullins, E., Milbourne, D., Petti, C., Doyle- Prestwich, B.M. and Meade, C. (2006) Potato in the age of biotechnology. Trends Plant Sci. 11, 254-260.
37. Murray, M.G. and Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8, 4321-4325.
38. Nicot, N., Hausman, J.F., Hoffmann, L. and Evers, D. (2005) Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J. Exp. Bot. 56, 2907-2914.
39. Osusky, M., Zhou, G., Osuska, L., Hancock, R.E., Kay, W.W. and Misra, S. (2000) Transgenic plants expressing cationic peptide chimeras exhibit broad-spectrum resistance to phytopathogens. Nat. Biotechnol. 18, 1162-1166.
40. Pearson, W.R. (1996) Comparison of DNA sequences with protein sequences. Methods Enzymol. 266, 227-258.
41. Peroni, D.G. and Boner, A.L. (1995) Sulfite sensitivity. Clin. Exp. Allergy, 25, 680-681.
42. Petersen, W., Umbeck, P., Hokanson, K. and Halsey, M. (2005) Biosafety considerations for selectable and scorable markers used in cassava (Manihot esculenta Crantz) biotechnology. Environ. Biosafety Res. 4, 89-102.
43. Pignocchi, C. and Foyer, C.H. (2003) Apoplastic ascorbate metabolism and its role in the regulation of cell signalling. Curr. Opin. Plant Biol. 6, 379-389.
44. Roessner, U., Wagner, C., Kopka, J., Trethewey, R.N. and Willmitzer, L. (2000) Simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry. Plant J. 23, 131-142.
45. Roessner, U., Luedemann, A., Brust, D., Fiehn, O., Linke, T., Willmitzer, L. and Fernie, A. (2001) Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems. Plant Cell, 13, 11-29.
46. Romano, E., Proite, K., Soares, A., Torres, A., Arieta, J., Jach, G., Mentaberry, A. and Monte, D. (2001) New binary vectors for plant co-transformation. Physiol. Mol. Biol. Plants, 7, 133-137.
47. Sheerman, S. and Bevan, M.W. (1988) A rapid transformation method for Solanum tuberosum using binary Agrobacterium tumefaciens vectors. Plant Cell Rep. 7, 13-16.
48. Singh, A.K., Praveen, S., Singh, B.P., Varma, A. and Arora, N. (2009) Safety assessment of leaf curl virus resistant tomato developed using viral derived sequences. Transgenic Res. 18, 877-887.
49. Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503-517.
50. Thygesen, P.W., Dry, I.B. and Robinson, S.P. (1995) Polyphenol oxidase in potato. A multigene family that exhibits differential expression patterns. Plant Physiol. 109, 525-531.
51. Tomás-Barberán, F.A. and Espín, J.C. (2001) Phenolic compounds and related enzymes as determinants of quality in fruits and vegetables. J. Sci. Food Agric. 81, 853-876.
52. Werij, J.S., Kloosterman, B., Celis-Gamboa, C., de Vos, C.H., America, T., Visser, R.G. and Bachem, C.W. (2007) Unravelling enzymatic discoloration in potato through a combined approach of candidate genes, QTL, and expression analysis. Theor. Appl. Genet. 115, 245-252.
53. Wesley, S.V., Helliwell, C.A., Smith, N.A., Wang, M.B., Rouse, D.T., Liu, Q., Gooding, P.S., Singh, S.P., Abbott, D., Stoutjesdijk, P.A., Robinson, S.P., Gleave, A.P., Green, A.G. and Waterhouse, P.M. (2001) Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J. 27, 581-590.
54. Whitaker, J.R. and Lee, C.Y. (1995) Recent advances in enzymatic browning. in "Enzymatic Browning and It's Prevention", in American Chemical Society Symposium Series 600, pp. 2-7, Washington, D. C.: American Chemical Society.
55. Zhuo, Q., Piao, J.H., Tian, Y., Xu, J. and Yang, X.G. (2009) Large-scale purification and acute toxicity of hygromycin B phosphotransferase. Biomed. Environ. Sci. 22, 22-27.