Biochemical characterization of extracellular polymeric substances extracted from an intertidal mudflat using a cation exchange resin

Biochemical Systematics and Ecology

Volumn 38, Issue 5, 2010, Pages 917-923

  Pierre, Guillaume ^a   Graber, Marianne ^a   Orvain, Francis ^b   Dupuy, Christine ^a   Maugard, Thierry ^a  

^a UNIVERSITÉ DE LA ROCHELLE   (France)

^b UNIVERSITÉ DE CAEN   (France)

Author keywords

Benthic ecology; Biochemical characterization; Biofilm; Extracellular compounds; In situ quantification

Indexed keywords

BENTHIC ENVIRONMENT; BIOFILM; BIOGEOCHEMISTRY; COLLOID; GLUCOSE; IN SITU MEASUREMENT; INTERTIDAL ENVIRONMENT; ION EXCHANGE; MUDFLAT; ORGANIC ACID; POLYMER; RESIN;

EID: 78650861047     PISSN: 03051978     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bse.2010.09.014     Document Type: Article

References (33)

1. Abdullahi A.S., Underwood G.J.C., Gretz M.R. Extracellular matrix assembly in diatoms (Bacillariophyceae). V. Environmental effects on polysaccharide synthesis in the model diatom, Phaeodactylum tricornutum. J. Phycol. 2006, 42:363-378.
2. Azerado J., Henriques M., Sillankorva S., Oliveira R. Extraction of exopolymers from biofilm: the protective effect of glutaraldehyde. Water Sci. Technol. 2003, 47:175-179.
3. Bellinger B.J., Abdullahi A.S., Gretz M.R., Underwood G.J.C. Biofilm polymers: relationship between carbohydrate biopolymers from estuarine mudflats and unialgal cultures of benthic diatoms. Aquat. Microb. Ecol. 2005, 38:169-180.
4. Bellinger B.J., Underwood G.J.C., Ziegler S.E., Gretz M.R. Significance of diatom-derived polymers in carbon flow dynamics within estuarine biofilms determined through isotopic enrichment. Aquat. Microb. Ecol. 2009, 55:169-187.
5. Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal. Biochem. 1973, 54:484-489.
6. Chiovitti A., Molino P., Crawford S.A., Ten R., Spurck T., Wetherbee R. The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides. Eur. J. Phycol 2004, 39:117-128.
7. Comte S., Guibaud G., Baudu M. Relations between extraction protocols for activated sludge extracellular polymeric substances (EPS) and EPS complexation properties Part I. Comparison of the efficiency of eight EPS extraction methods. Enzyme Microb. Technol. 2006, 38:237-245.
8. Craigie J.S., Wen Z.C., van der Meer J.P. Interspecific, intraspecific and nutrionally-determinated variations in the composition of agars from Gracilaria spp. Bot. Mar. 1984, 27:55-61.
9. de Brouwer J.F.C., Stal L.J. Short-term dynamics in microphytobenthos distribution and associated extracellular carbohydrates in surface sediments of an intertidal mudflat. Mar. Ecol. Prog. Ser. 2001, 218:33-44.
10. de Brouwer J.F.C., Stal L.J. Does warm-water extraction of benthic diatoms yield extracellular polymeric substances or does it extract intracellular chrysolaminaran?. Eur. J. Phycol. 2004, 39:129-131.
11. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1956, 28:350-356.
12. Filisetti-Cozzi T.M., Carpita N.C. Measurement of uronic acids without interference from neutral sugars. Anal. Biochem. 1991, 197:157-162.
13. Frølund B., Palmgren R., Keiding K., Nielsen P.H. Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Res. 1996, 30:1749-1758.
14. Giroldo D., Vieira A.A.H., Paulsen B.S. Relative increase of deoxy sugars during microbial degradation of an extracellular polysaccharide released by a tropical freshwater Thalassiosira sp. (Bacillariophyceae). J. Phycol. 2003, 39:1109-1115.
15. Hanlon A.R.M., Bellinger B., Haynes K., Xiao G., Hofmann T.A., Gretz M.R., Ball A.S., Osborn M., Underwood G.J.C. Dynamics of extracellular polymeric substances (EPS) production and loss in an estuarine, diatom-dominated, microbial biofilm over a tidal emersion-immersion period. Limnol. Oceanogr. 2006, 51:79-93.
16. Hofmann T., Hanlon A.R.M., Taylor J.D., Ball A.S., Osborn A.M., Underwood G.J.C. Dynamics and compositional changes in extracellular carbohydrates in estuarine sediments during degradation. Mar. Ecol. Prog. Ser. 2009, 379:45-58.
17. Jaques L.B., Ballieux R.E., Dietrich C.P., Kavanagh L.W. A microelectrophoresis method for heparin. Can. J. Physiol. Pharmacol. 1968, 46:351-360.
18. Orvain F., Galois R., Barnard C., Sylvestre A., Blanchard G., Sauriau P.G. Carbohydrate production in relation to microphytobenthic biofilm development: an integrated approach in a tidal mesocosm. Microb. Ecol. 2003, 45:237-251.
19. Perkins R.G., Honeywill C., Consalvey M., Austin H.A., Tolhurst T.J., Paterson D.M. Changes in microphytobenthic chlorophyll a and EPS resulting from sediment compaction due to de-watering: opposing patterns in concentration and content. Cont. Shelf Res. 2003, 23:575-586.
20. Perkins R.G., Paterson D.M., Sun H., Watson J., Player M.A. Extracellular polymeric substances: quantification and use in erosion experiments. Continent Shelf Res. 2004, 24:1623-1635.
21. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 1985, 150:76-85.
22. Spears B.M., Saunders J.E., Davidson I., Paterson D.M. Microalgal sediment biostabilisation along a salinity gradient in the Eden Estuary, Scotland: unraveling a paradox. Mar. Freshwater Res. 2008, 59:313-321.
23. Staats N., de Winder B., Stal L.J., Mur L.R. Isolation and characterization of extracellular polysaccharides from the epipelic diatoms Cylindrotheca clostrerium and Navicula salinarum. Eur. J. Phycol. 1999, 34:161-169.
24. Staats N., Stal L.J., Mur L.R. Exopolysaccharide production by the epipelic diatom Cylindrotheca clostherium: effects of nutrient conditions. J. Exp. Mar. Biol. Ecol. 2000, 249:13-27.
25. Stal L.J. Microphytobenthos, their extracellular polymeric substances, and the morphogenesis of intertidal sediments. Geomicrobiol. J. 2003, 20:463-478.
26. Stal L.J., Défarge C. Structure and dynamics of exopolymers in an intertidal diatom biofilm. Geomicrobiol. J. 2005, 22:341-352.
27. Stoodley P., Sauer K., Davies D.G., Costerton J.W. Biofilms as complex differentiated communities. Annu. Rev. Microbiol. 2002, 56:187-209.
28. Takahashi E., Ledauphin J., Goux D., Orvain F. Optimizing extraction of extracellular polymeric substances (EPS) from benthic diatoms: comparison of the efficiency of six EPS extraction methods. Mar. Freshw. Res. 2009, 60:1201-1210.
29. Underwood G.J.C., Paterson D.M. The importance of extracellular carbohydrate production by marine epipelic diatoms. Adv. Bot. Res. 2003, 40:184-240.
30. Underwood G.J.C., Smith D.J. Predicting epipelic diatom exopolymer concentrations in intertidal sediments from sediment chl. a. Microb. Ecol. 1998, 35:116-125.
31. van Duyl F.C., de Winder B., Kop A.J., Wollenzien U. Tidal coupling between carbohydrate concentrations and bacterial activities in diatom-inhabited intertidal mudflats. Mar. Ecol. Prog. Ser. 1999, 191:19-32.
32. Witvrouw M., De Clercq E. Sulfated polysaccharides extracted from sea algae as potential antiviral drugs. Gen. Pharmac. 1997, 29:497-511.
33. Zhou J., Mopper K., Passow U. The role of surface-active carbohydrates in the formation of transparent exopolymer particles by bubble adsorption of seawater. Limnol. Oceanogr 1998, 43:1860-1871.