Comparison of the colony formation and crystal violet cell proliferation assays to determine cellular radiosensitivity in a repair-deficient MCF10A cell line

Radiation Measurements

Volumn 46, Issue 1, 2011, Pages 72-75

  Vandersickel, Veerle ^a   Slabbert, Jacobus ^b   Thierens, Hubert ^a   Vral, Anne ^a  

^a GHENT UNIVERSITY   (Belgium)

^b ITHEMBA LABS   (South Africa)

Author keywords

Colony formation assay; Crystal violet cell proliferation assay; Radiosensitivity

Indexed keywords

CELL LINES; CELLULAR RADIOSENSITIVITY; CLONOGENIC ASSAYS; COLONY FORMATION; COLONY FORMATION ASSAY; CRYSTAL VIOLET; CRYSTAL VIOLET CELL PROLIFERATION ASSAY; IN-VITRO; MODIFYING FACTORS; RADIATION DOSE; RADIOSENSITIVITY; SURVIVAL CURVES; SURVIVING FRACTIONS; TUMOR RESPONSE;

CELL CULTURE; CELL PROLIFERATION;

ASSAYS;

EID: 78650839253     PISSN: 13504487     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.radmeas.2010.08.003     Document Type: Article

References (18)

1. D. Banasiak, A.R. Barnetson, R.A. Odell, H. Mameghan, and P.J. Russell Comparison between the clonogenic, MTT, and SRB assays for determining radiosensitivity in a panel of human bladder cancer cell lines and a ureteral cell line Radiat. Oncol. Investig. 7 1999 77 85
2. M.H. Bourguignon, P.A. Gisone, M.R. Perez, S. Michelin, D. Dubner, M.D. Giorgio, and E.D. Carosella Genetic and epigenetic features in radiation sensitivity. Part II: implications for clinical practice and radiation protection Eur. J. Nucl. Med. Mol. Imaging 32 2005 351 368
3. K. Chiba, K. Kawakami, and K. Tohyama Simultaneous evaluation of cell viability by neutral red, MTT and crystal violet staining assays of the same cells Toxicol. In Vitro 12 1998 251 258
4. A. Courdi, N. Brassart, J. Herault, D. Mari, and P. Chauvel The RBE of fast neutrons for in vitro inactivation of human tumour cells determined by the ratio of mean inactivation doses Acta Oncol. 35 1996 237 242
5. P. Cross, E. Marshall, B. Baguley, G. Finley, J. Matthews, and W. Wilson Proliferative assays for the assessment of radiosensitivity of tumor cell lines using 96-well microcultures Radiat. Oncol. Investig. 1 1994 261 269
6. J. Deacon, M.J. Peckham, and G.G. Steel The radioresponsiveness of human tumours and the initial slope of the cell survival curve Radiother. Oncol. 2 1984 317 323
7. B. Fertil, and E.P. Malaise Inherent cellular radiosensitivity as a basic concept for human tumor radiotherapy Int. J. Radiat. Oncol. Biol. Phys. 7 1981 621 629
8. B. Fertil, and E.P. Malaise Intrinsic radiosensitivity of human cell lines is correlated with radioresponsiveness of human tumors: analysis of 101 published survival curves Int. J. Radiat. Oncol. Biol. Phys. 11 1985 1699 1707
9. E.J. Hall Radiobiology for the Radiobiologist third ed. 1988 J.B. Lipincott Company Philadelphia
10. E. LaTorre Travis Primer of Medical Radiobiology second ed. 1989 Mosby-Year Book, Inc. St Louis
11. S. Lehnert Biomolecular Action of Ionizing Radiation 2008 Taylor and Francis Group, LLC
12. K.N. Prasad Handbook of Radiobiology second ed. 1995 CRC Press, Inc.
13. T.T. Puck, and P.I. Marcus Action of x-rays on mammalian cells J. Exp. Med. 103 1956 653 666
14. T.T. Puck, D. Morkovin, P.I. Marcus, and S.J. Cieciura Action of x-rays on mammalian cells. II. Survival curves of cells from normal human tissues J. Exp. Med. 106 1957 485 500
15. 60Co γ-rays Strahlenther. Onkol. 172 1996 567 572
16. M. Tubiana, J. Dutreix, and A. Wambersie Introduction to Radiobiology 1990 Taylor and Francis London
17. V. Vandersickel, M. Mancini, E. Marras, P. Willems, J. Slabbert, J. Philippé, E. Deschepper, H. Thierens, G. Perletti, and A. Vral Lentivirus-mediated RNA interference of Ku70 to enhance radiosensitivity of human mammary epithelial cells Int. J. Radiat. Biol. 86 2010 114 124
18. C.M. West Intrinsic radiosensitivity as a predictor of patient response to radiotherapy Br. J. Radiol. 68 1995 827 837