Generation of an anti-NAGase single chain antibody and its application in a biosensor-based assay for the detection of NAGase in milk

Journal of Immunological Methods

Volumn 364, Issue 1-2, 2011, Pages 14-20

  Welbeck, Katherine ^a   Leonard, Paul ^a   Gilmartin, Niamh ^a   Byrne, Barry ^a   Viguier, Caroline ^a,b   Arora, Sushrut ^a   O'Kennedy, Richard ^a  

^a DUBLIN CITY UNIVERSITY   (Ireland)

^b Enfer Scientific   (Ireland)

Author keywords

Immunoassay; NAGase; ScFv; SPR

Indexed keywords

ENZYME ANTIBODY; N ACETYL BETA GLUCOSAMINIDASE; N ACETYL BETA GLUCOSAMINIDASE ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; ARTICLE; BIOSENSOR; ENZYME IMMOBILIZATION; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOASSAY; MILK; PRIORITY JOURNAL; SURFACE PLASMON RESONANCE;

ACETYLGLUCOSAMINIDASE; ANIMALS; AUTOMATION, LABORATORY; BIOLOGICAL MARKERS; BIOSENSING TECHNIQUES; CATTLE; EARLY DIAGNOSIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; MASTITIS, BOVINE; MILK; REFERENCE STANDARDS; SINGLE-CHAIN ANTIBODIES; SURFACE PLASMON RESONANCE;

BOVINAE;

EID: 78650823191     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.09.019     Document Type: Article

References (13)

1. Akerstedt M., Bjorck L., Waller K.P., Sternesjo A. Biosensor assay for determination of haptoglobin in bovine milk. J. Dairy Res. 2006, 73:299.
2. Gillis E.H., Gosling J.P., Sreenan J.M., Kane M. Development and validation of a biosensor-based immunoassay for progesterone in bovine milk. J. Immunol. Meth. 2002, 267:131.
3. Gronlund U., Hulten C., Eckersall P.D., Hogarth C., Persson Waller K. Haptoglobin and serum amyloid A in milk and serum during acute and chronic experimentally induced Staphylococcus aureus mastitis. J. Dairy Res. 2003, 70:379.
4. Gustavsson E., Degelaen J., Bjurling P., Sternesjo A. Determination of beta-lactams in milk using surface plasmon resonance based biosensor. J. Agric. Food Chem. 2004, 52:2791.
5. Hiss S., Mielenz M., Bruckmaier R.M., Sauerwein H. Haptoglobin concentrations in blood and milk after endotoxin challenge and quantification of mammary Hp mRNA expression. J. Dairy Sci. 2004, 87:3778.
6. Kitchen B.J., Middleton G., Salmon L. Bovine milk N-acetyl-β-D-glycosamidase and its significance in the detection of abnormal udder secretions. J. Dairy Res. 1978, 45:15.
7. Larsen T. Determination of lactate dehydrogenase (LDH) activity in milk by a fluorometric assay. J. Dairy Res. 2005, 72:209.
8. Murphy M., Jason-Moller L., Bruno J. Using Biacore to measure the binding kinetics of an antibody-antigen interaction. Curr. Protoc. Protein Sci. 2006, 19.14.1-19.14.17 (Chapter 19. Unit 19.14).
9. Pemberton R.M., Hart J.P., Mottram T.T. An assay for the enzyme N-acetyl-β-D-glycosamidase (NAGase) based on electrochemical detection using screen printed carbon electrodes (SPCEs). Analyst (Lond.) 2001, 126:1866.
10. Piccinini R., Binda E., Belotti M., Dapra V., Zecconi A. Evaluation of milk components during whole lactation in healthy quarters. J. Dairy Res. 2007, 74:226.
11. Szczubial M., Dabrowsk R., Kankofer M., Bochniarz M., Albera E. Concentration of serum amyloid A and activity of ceruloplasmin in milk from cows with clinical and subclinical mastitis. Bull. Vet. Inst. Pulawy 2008, 52:391.
12. Tamburini A., Bava L., Piccinini R., Zecconi A., Zucali M., Sandrucci A. Milk emission and udder health status in primiparous dairy cows during lactation. J. Dairy Res. 2009, 77:13.
13. Viguier C., Arora S., Gilmartin N., Welbeck K., O'Kennedy R. Mastitis detection: Current trends and future perspectives. Trends Biotechnol. 2009, 27:486.