Characterization of a Paenibacillus woosongensis β-xylosidase/ α-arabinofuranosidase produced by recombinant Escherichia coli

Journal of Microbiology and Biotechnology

Volumn 20, Issue 12, 2010, Pages 1711-1716

  Kim, Yeon A ^a   Yoon, Ki Hong ^a  

^a Woosong University   (South Korea)

Author keywords

xylosidase arabinofuranosidase; Characterization; Paenibacillus woosongensis; Recombinant escherichia coli

Indexed keywords

1,4 NITROPHENYL ALPHA ARABINOFURANOSIDE; 1,4 NITROPHENYL BETA XYLOPYRANOSIDE; ALPHA ARABINOFURANOSIDASE; GENOMIC DNA; GLYCOSIDASE; HEXOSE; NUCLEOSIDE; PENTOSE; UNCLASSIFIED DRUG; XYLAN 1,4 BETA XYLOSIDASE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BACILLUS; BACILLUS CELLULOSILYTICUS; BACTERIAL GENE; BACTERIUM MUTANT; CARBOXY TERMINAL SEQUENCE; CLOSTRIDIUM; CLOSTRIDIUM STERCORARIUM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SUBSTRATE; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXPRESSION VECTOR; GENOME SIZE; HYDROLYSIS; MICHAELIS CONSTANT; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PAENIBACILLUS; PAENIBACILLUS WOOSONGENSIS; PH; PROTEIN FAMILY; SEQUENCE HOMOLOGY; STRUCTURAL GENE; TEMPERATURE; XYLC GENE;

ARABINOSE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA, BACTERIAL; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GLYCOSIDE HYDROLASES; GLYCOSIDES; HEXOSES; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; PAENIBACILLUS; PENTOSES; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SODIUM DODECYL SULFATE; TEMPERATURE; XYLOSIDASES;

CLOSTRIDIUM STERCORARIUM; ESCHERICHIA COLI; PAENIBACILLUS;

EID: 78650820845     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1010.10040     Document Type: Article

References (21)

1. Brüx, C., A. Ben-David, D. Shallom-Shezifi, M. Leon, K. Niefind, G. Shoham, Y. Shoham, and D. Schomburg. 2006. The structure of an inverting GH43 β-xylosidase from Geobacillus stearothermophilus with its substrate reveals the role of the three catalytic residues. J. Mol. Biol. 359: 97-109.
2. Contreras, L. M., J. Gómez, J. Prieto, J. M. Clemente-Jiménez, F. J. Las Heras-Vázquez, F. Rodríguez-Vico, F. J. Blanco, and J. L. Neira. 2008. The family 52 β-xylosidase from Geobacillus stearothermophilus is a dimer: Structural and biophysical characterization of a glycoside hydrolase. Biochim. Biophys. Acta 1784: 1924-1934.
3. Dodd, D., S. A. Kocherginskaya, M. A. Spies, K. E. Beery, C. A. Abbas, R. I. Mackie, and I. K. Cann. 2009. Biochemical analysis of a β-D-xylosidase and a bifunctional xylanase-ferulic acid esterase from a xylanolytic gene cluster in Prevotella ruminicola 23. J. Bacteriol. 191: 3328-3338.
4. Jordan, D. B., X. L. Li, C. A. Dunlap, T. R. Whitehead, and M. A. Cotta. 2007. Structure-function relationships of a catalytically efficient β-D-xylosidase. Appl. Biochem. Biotechnol. 141: 51-76.
5. La Grange, D. C., I. S. Pretorius, and W. H van Zyl. 1997. Cloning of the Bacillus pumilus β-xylosidase gene (xynB) and its expression in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 47: 262-266.
6. Lama, L., V. Calandrelli, A. Gambacorta, and B. Nicolaus. 2004. Purification and characterization of thermostable xylanase and β-xylosidase by the thermophilic bacterium Bacillus thermantarcticus. Res. Microbiol. 155: 283-289.
7. Lee, J.-C. and K.-H. Yoon. 2008. Paenibacillus woosongensis sp. nov., a xylanolytic bacterium isolated from forest soil. Int. J. Syst. Evol. Microbiol. 58: 612-616.
8. Lee, Y E. and J. G. Zeikus. 1993. Genetic organization, sequence and biochemical characterization of recombinant β- xylosidase from Thermoanaerobacterium saccharolyticum strain B6A-RI. J. Gen. Microbiol. 139: 1235-1243.
9. Luan, H., Y. Hu, X. Liu, D. Hao, and L. Yang. 2008. Purification and characterization of a β-D-xylosidase from Leifsonia shinshuensis DICP 16. Sheng Wu Gong Cheng Xue Bao 24: 867-873.
10. Quintero, D., Z. Velasco, E. Hurtado-Gómez, J. L. Neira, and L. M. Contreras. 2007. Isolation and characterization of a thermostable β-xylosidase in the thermophilic bacterium Geobacillus pallidus. Biochim. Biophys. Acta 1774: 510-518.
11. Sakka, K., K. Yoshikawa, Y. Kojima, S. Karita, K. Ohmiya, and K. Shimada. 1993. Nucleotide sequence of the Clostridium stercorarium xylA gene encoding a bifunctional protein with β- D-xylosidase and α-L-arabinofuranosidase activities, and properties of the translated product. Biosci. Biotechnol. Biochem. 57: 268-272.
12. Shao, W. and J. Wiegel. 1992. Purification and characterization of a thermostable β-xylosidase from Thermoanaerobacter ethanolicus. J. Bacteriol. 174: 5848-5853.
13. Suryani, K. T., K. Sakka, and K. Ohmiya. 2004. Sequencing and expression of the gene encoding the Clostridium stercorarium β-xylosidase Xyl43B in Escherichia coli. Biosci. Biotechnol. Biochem. 68: 609-614.
14. Suzuki, T., E. Kitagawa, F. Sakakibara, K. Ibata, K. Usui, and K. Kawai. 2001. Cloning, expression, and characterization of a family 52 β-xylosidase gene (xysB) of a multiple-xylanaseproducing bacterium, Aeromonas caviae ME-1. Biosci. Biotechnol. Biochem. 65: 487-494.
15. Tsujibo, H., C. Takada, A. Tsuji, M. Kosaka, K. Miyamoto, and Y. Inamori. 2001. Cloning, sequencing, and expression of the gene encoding an intracellular β-D-xylosidase from Streptomyces thermoviolaceus OPC-520. Biosci. Biotechnol. Biochem. 65: 1824-1831.
16. Valenzuela, S. V., P. Diaz, and F. I. Javier Pastor. 2010. Recombinant expression of an alkali stable GH10 xylanase from Paenibacillus barcinonensis. J. Agric. Food Chem. 58: 4814-4818.
17. Waeonukul, R., P. Pason, K. L. Kyu, K. Sakka, A. Kosugi, Y. Mori, and K. Ratanakhanokchai. 2009. Cloning, sequencing, and expression of the gene encoding a multidomain endo-β-1,4-xylanase from Paenibacillus curdlanolyticus B-6, and characterization of the recombinant enzyme. J. Microbiol. Biotechnol. 19: 277-285.
18. Wagschal, K., C. Heng, C. C. Lee, G. H. Robertson, W. J. Orts, and D. W. Wong. 2009. Purification and characterization of a glycoside hydrolase family 43 β-xylosidase from Geobacillus thermoleovorans IT-08. Appl. Biochem. Biotechnol. 155: 304-313.
19. Xu, W. Z., Y. Shima, S. Negoro, and I. Urabe, 1991. Sequence and properties of β-xylosidase from Bacillus pumilus IPO. Contradiction of the previous nucleotide sequence. Eur. J. Biochem. 202: 1197-1203.
20. Xue, Y. and W. Shao. 2004. Expression and characterization of a thermostable β-xylosidase from the hyperthermophile, Thermotoga maritima. Biotechnol. Lett. 26: 1511-1515.
21. Zhang, J. and L. R. Lynd. 2010. Ethanol production from paper sludge by simultaneous saccharification and co-fermentation using recombinant xylose-fermenting microorganisms. Biotechnol. Bioeng. 107: 235-244.