Enzymatic antioxidant defense in isolated rat hepatocytes exposed to cadmium

Polish Journal of Veterinary Sciences

Volumn 13, Issue 4, 2010, Pages 673-679

  Skrzycki, M ^a   Czeczot, H ^a   Majewska, M ^a   Podsiad, M ^a   Karlik, W ^b   Grono, D ^b   Wiechetek, M ^b  

^a MEDICAL UNIVERSITY OF WARSAW   (Poland)

^b WARSAW UNIVERSITY OF LIFE SCIENCES   (Poland)

Author keywords

Antioxidant enzymes; Cadmium; Isolated rat hepatocytes; Lipid peroxidation (tbars); Oxidative stress; Reduced glutathione (gsh)

Indexed keywords

ANTIOXIDANT; CADMIUM CHLORIDE; GLUTATHIONE; THIOBARBITURIC ACID REACTIVE SUBSTANCE;

ANIMAL; ARTICLE; CELL CULTURE; DOSE RESPONSE; DRUG EFFECT; ENZYMOLOGY; LIVER CELL; MALE; METABOLISM; RAT; TIME; WISTAR RAT;

ANIMALS; ANTIOXIDANTS; CADMIUM CHLORIDE; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; GLUTATHIONE; HEPATOCYTES; MALE; RATS; RATS, WISTAR; THIOBARBITURIC ACID REACTIVE SUBSTANCES; TIME FACTORS;

RATTUS;

EID: 78650818063     PISSN: 15051773     EISSN: None     Source Type: Journal    
DOI: 10.2478/v10181-010-0002-7     Document Type: Article

References (39)

1. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay
2. applicable to acrylamide gels. Anal Biochem 44: 276-287.
3. Bojakowska K (2006) Evaluation of cytotoxicity of cadmium compounds: in vitro studies on rat and equine isolated hepatocytes. Doctoral thesis, Warsaw, Warsaw University of Life Sciences, Poland.
4. Borzçcka K, Bekala A, Grono D, Glowala A, Wiechetek M (2004) Evaluation of cadmium cytotoxicity in isolated hepatocytes: effect of plating time, medium composition and cadmium chemical compounds. 13th Workshop of the European Society of Toxicology In Vitro "INVITOX", Zegrze, pp 32.
5. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal Biochem 72: 248-254.
6. Brzóska MM, Moniuszko-Jakoniuk J (1998) The influence of calcium content in the diet on accumulation and toxicity of cadmium in the organism. Arch Toxicol 72: 63-73.
7. Casalino E, Calzaretti G, Sblano C, Landriscina C (2002) Molecular inhibitory mechanisms of antioxidant enzymes in rat liver and kidney by cadmium. Toxicology 179: 37-50.
8. European Food Safety Authority (2009) Cadmium in food. Scientific Opinion of the panel on contaminants in the Food Chain. EFSA J 980: 1-139.
9. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82: 70-77.
10. Fariss MW (1991) Cadmium toxicity: unique cytoprotective properties of alpha tocopheryl succinate in hepatocytes. Toxicology 69: 63-77.
11. Golberg DM, Spooner RJ, Bregmayer HV (1983) Glutathione reductase. Methods Enzymatic Analysis. Verlag Chemie, Weinheim 3: 258-265.
12. Góth L (1999) A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta 196: 143-151.
13. Habig WH, Pabst M, Jaoby WB (1974) Glutathione S-transferase: The first step in mercapturic acid formation. J Biol Chem 249: 7130-7139.
14. Hayes JD, McLellan LI (1999) Glutathione and glutathione-dependent enzymes represent a co-ordinately regulated defence against oxidative stress. Free Radic Res 31: 273-300.
15. International Agency for Research on Cancer (IARC) (1993) Beryllium, cadmium, mercury, and exposure in the glass manufacturing industry. Monographs on the evaluation of the carcinogenic risks to humans. IARC Scientific Publications, Lyon, 58, pp 119-237.
16. Invitox Protocol Number 20. Isolation of Rat Hepatocytes. Invitox, Protocol ERGATT/FRAME Data bank of In Vitro Techniques in Toxicology (1991)
17. Järup L, Berglund M, Elinder CG, Nordberg G, Vahter M (1998) Health effects of cadmium exposure: a review of the literature and a risk estimate. Scand J Work Environ Health 24: 1-51.
18. Jurczuk M, Brzóska MM, Moniuszko-Jakoniuk J, Galazyn-Sidorczuk M, Kulikowska-Karpińska E (2004) Antioxidant enzymes activity and lipid peroxidation in liver and kidney of rats exposed to cadmium and ethanol. Food Chem Toxicol 42: 429-438.
19. Kehrer JP (1993) Free radicals as mediators of tissue injury and disease. Crit Rev Toxicol 23: 21-48.
20. Kolacz R, Dobrzański Z, Bodak E (1996) Bio-accumulation of Cd, Pb and Hg in animal tissues. Veterinary Medicine (Polish) 52: 686-691.
21. Oberley LW, Spitz DR (1984) Assay of superoxide dismutase activity in tumor tissue. Methods Enzymol 105: 457-464.
22. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351-358.
23. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70: 158-169.
24. Pastore A, Federici G, Bertini E, Piemonte F (2003) Analysis of glutathione: implication in redox and detoxification. Clin Chim Acta 33: 19-39.
25. +2. Toxicology 143: 263-273.
26. Pourahmad J, O'Brien PJ, Jokar F, Daraei B (2003) Carcinogenic metal induced reactive oxygen species formation in hepatocytes. Toxicol in Vitro. 17: 803-810.
27. Robertson JD, Orrenius S (2000) Molecular mechanisms of apoptosis induced by cytotoxic chemicals. Crit Rev Toxicol 30: 609-627.
28. Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 25: 192-205.
29. Sen CK (2000) Cellular thiols and redox-regulated signal transduction. Curr Top Cell Regul 36: 1-30.
30. Shaikh ZA, Vu TT, Zaman K (1999) Oxidative stress as a mechanism of chronic cadmium-induced hepatotoxicity and renal toxicity and protection by antioxidants. Toxicol Appl Pharmacol 154: 256-263.
31. Sies H (1999) Glutathione and its role cellular functions. Free Radie Biol Med 27: 916-921.
32. Stohs SJ, Bagchi D, Hassoun E, Bagchi M (2001) Oxidative mechanisms in the toxicity of chromium and cadmium ions. J Environ. Pathol Toxicol Oncol 20: 77-88.
33. Tennant RJ (1964) Evaluation of trypan blue technique for determination of cell viability. Transplantation 2: 685-694.
34. Ungar K (1991) Invitox Protocol Number 20. Isolation of Rat Hepatocytes. Invitox, Protocol ERGATT/FRAME Data bank of In Vitro Techniques in Toxicology.
35. Waalkes MP (2003) Cadmium carcinogenesis. Mutat Res 533: 107-120.
36. Waisberg M, Joseph P, Hale B, Beyersmann D (2003) Molecular and cellular mechanisms of cadmium carcinogenesis. Toxicology 192: 95-117.
37. Wang Y, Fang J, Leonard SS, Rao KM (2004) Cadmium inhibits the electron transfer chain and induces reactive oxygen species. Free Radic Biol Med 11: 1434-1443.
38. Wendel A (1981) Glutathione peroxidase. Methods Enzymol 77: 325-333.
39. Williams GM, Jeffrey AM (2000) Oxidative DNA damage: endogenous and chemically induced. Regul Toxicol Pharmacol 32: 283-292.