Novel GmFAD2-1b mutant alleles created by reverse genetics induce marked elevation of oleic acid content in soybean seeds in combination with GmFAD2-1a mutant alleles

Breeding Science

Volumn 60, Issue 4, 2010, Pages 419-425

  Hoshino, Tomoki ^a   Takagi, Yutaka ^a   Anai, Toyoaki ^a  

^a SAGA UNIVERSITY   (Japan)

Author keywords

Fad2; Glycine max; High oleic acid; Marker assisted; Mutant library; Reverse genetics; Tilling

Indexed keywords

GLYCINE MAX;

EID: 78650800093     PISSN: 13447610     EISSN: 13473735     Source Type: Journal    
DOI: 10.1270/jsbbs.60.419     Document Type: Article

References (28)

1. Anai, T., T. Yamada, T. Kinoshita, S.M. Rahman and Y. Takagi (2005) Identification of corresponding genes for three low-α-linolenic acid mutants and elucidation of their contribution to fatty acid biosynthesis in soybean seed. Plant Sci. 168: 1615-1623.
2. Anai, T., T. Yamada, R. Hideshima, T. Kinoshita, S.M. Rahman and Y.Takagi (2008) Two high-oleic-acid soybean mutants, M23 and KK21, have disrupted microsomal omega-6 fatty acid desaturase, encoded by GmFAD2-1a. Breed. Sci. 58: 447-452.
3. Buhr, T., S. Sato, F. Ebrahim, A. Xing, Y. Zhou, M. Mathiesen, B. Schweiger, A. Kinney, P. Staswick and T. Clemente (2002) Ribozyme termination of RNA transcripts down-regulate seed fatty acid genes in transgenic soybean. Plant J. 30: 155-163.
4. Clemente, T.E. and E.B. Cahoon (2009) Soybean oil: genetic approaches for modification of functionality and total content. Plant Physiol. 151: 1030-1040.
5. Davies, H., E. Dicks, P. Stephens, C. Cox, J. Teague, C. Greenman, G. Bignell, S. O'Meara, S. Edkins, A. Parker et al. (2006) High throughput DNA sequence variant detection by conformation sensitive capillary electrophoresis and automated peak comparison. Genomics 87: 427-432.
6. Dierking, E.C. and K.D. Bilyeu (2009) New sources of soybean seed meal and oil composition traits identified through TILLING. BMC Plant Biol. 9: 89.
7. Gady, A., F. Hermans, M. Van de Wal, E. van Loo, R. Visser and C. Bachem (2009) Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations. Plant Methods 5: 13.
8. Henikoff, S., B.J. Till and L. Comai (2004) TILLING. Traditional mutagenesis meets functional genomics. Plant Physiol. 135: 630-636.
9. Heppard, E.P., A.J. Kinney, K.L. Stecca and G.H. Miao (1996) Developmental and growth temperature regulation of two different microsomal omega-6 desaturase genes in soybeans. Plant Physiol. 110: 311-319.
10. Hyten, L.D., Q. Song, Y. Zhu, I.-Y. Choi, L.R. Nelson, M.J. Costa, E.J. Specht, R.C. Shoemaker and B.P. Cregan (2006) Impact of genetic bottlenecks on soybean genome diversity. Proc. Natl. Acad. Sci. 103: 16666-16671.
11. Iizasa, E. and Y. Nagano (2006) Highly efficient yeast-based in vivo DNA cloning of multiple DNA fragments and the simultaneous construction of yeast/Escherichia coli shuttle vectors. Biotechniques 40: 79-83.
12. Kinney, A.J. and S. Knowlton (1998) Designer oils: high oleic acid soybean. In: Harander, S. and S. Roller (eds.) Genetic Engineering for Food Industry: a Strategy for Food Quality Improvement, Blackie Academic, London, pp. 193-213.
13. Murray, M.G. and W.F. Thompson (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321-4325.
14. Olhoft, P.M., L.E. Flagel, C.M. Donovan and D.A. Somers (2002) Efficient soybean transformation using hygromycinB selection in the cotyledonary-node method. Planta 216: 723-735.
15. Pham, A.T., J.D. Lee, G. Shannon and K. Bilyeu (2010) Mutant alleles of FAD2-1a and FAD2-1b combine to produce soybeans with the high oleic acid seed oil trait. BMC Plant Biol. 10: 195.
16. Rahman, S.M., T. Kinoshita, T. Anai and Y. Takagi (2001) Combining ability in loci for high oleic and low linolenic acids in soybean. Crop Sci. 41: 26-29.
17. Rigola, D., J. van Oeveren, A. Janssen, A. Bonné, H. Schneiders, H. van der Poel, N. van Orsouw, R. Hogers, M. de Both and M. van Eijk (2009) High-throughput detection of induced mutations and natural variation using KeyPoint™ technology. PLoS ONE 4: e4761.
18. Santarem, E.R. and J.J. Finer (1999) Transformation of soybean (Glycine max (L.) Merrill) using proliferative embryogenic tissue maintained on semi-solid medium. In Vitro Cell Dev. Biol. 35: 451-455.
19. Schlueter, J.A., I.F. Vasylenko-Saders, S. Deshpande, J. Yi, M. Siegfried, B.A. Roe, S.D. Schlueter, B.E. Scheffler and R.C. Shoemaker (2007) The FAD2 gene family of soybean: insights into the structural and functional of a paleopolyploid genome. Crop Sci. 47: S14-S26.
20. Schmutz, J., S.B. Cannon, J. Schlueter, J. Ma, M. Mitros, W. Nelson, D.L. Hyten, Q. Song, J.J. Thelen, J. Cheng et al. (2010) Genome sequence of the palaeopolyploid soybean. Nature 463: 178-183.
21. Soy Stats (2009) The American Soybean Association. http://www.soystats.com/
22. Takagi, Y. and S. Arima (2011) Selection of high seed-yielding strains from high oleic acid mutants of soybean having multiple alleles of ol at the Ol locus. Jpn. J. Crop Sci. 80: 73-76.
23. Takagi, T. and S.M. Rahman (1996) Inheritance of high oleic acid content in the seed oil of soybean mutant M23. Theor. Appl. Genet. 92: 179-182.
24. Tang, G.Q., W.P. Novitzky, H.C. Griffin, S.C. Huber and R.E. Dewey (2005) Oleate desaturase enzymes of soybean: evidence of regulation through differential stability and phosphorylation. Plant J. 44: 433-446.
25. Thompson, J.D., D.G. Higgins and T.J. Gibson (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680.
26. Till, B.J., S.H. Reynolds, E.A. Greene, C.A. Codomo, L.C. Enns, J.E. Johnson, C. Burtner, A.R. Odden, K. Young, N.E. Taylor et al. (2003) Large-scale discovery of induced point mutations with high-throughput TILLING. Genome Res. 13: 524-530.
27. Vossen, R.H.A.M., E. Aten, A. Roos and J.T. Den Dunnen (2009) Highresolution melting analysis (HRMA)-More than just sequence variant screening. Human Mutation 30: 860-866.
28. Yadav, N.S., A. Wierzbicki, M. Aegerter, C.S. Caster, L. Perez-Grau, A.J. Kinney, W.D. Hitz, J.R. Booth, B. Schweiger and K.L. Stecca (1993) Cloning of higher plant omega-3 fatty acid desaturase. Plant Physiol. 103: 467-476.