Base substitutions at scissile bond sites are sufficient to alter RNA-binding and cleavage activity of RNase III

FEMS Microbiology Letters

Volumn 315, Issue 1, 2011, Pages 30-37

  Kim, Kyungsub ^a   Sim, Se Hoon ^a   Jeon, Che Ok ^a   Lee, Younghoon ^b   Lee, Kangseok ^a  

^a Chung Ang University   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

bdm; Endoribonuclease; RNase III; Rnc

Indexed keywords

MESSENGER RNA; RIBONUCLEASE III;

ARTICLE; BINDING AFFINITY; BINDING SITE; CHEMICAL BOND; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; GENE CONSTRUCT; GENE MUTATION; HYDROLYSIS; IN VITRO STUDY; IN VIVO STUDY; MUTANT; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; RNA BINDING; RNA CLEAVAGE; RNA SEQUENCE; RNA STABILITY; SCISSILE BOND; WILD TYPE;

BASE SEQUENCE; BINDING SITES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEIC ACID CONFORMATION; PROTEIN BINDING; RIBONUCLEASE III; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, BACTERIAL; RNA, MESSENGER;

EID: 78650799603     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02169.x     Document Type: Article

References (20)

1. Amarasinghe AK, Calin-Jageman I, Harmouch A, Sun W & Nicholson AW (2001) Escherichia coli ribonuclease III: affinity purification of hexahistidine-tagged enzyme and assays for substrate binding and cleavage. Method Enzymol 342: 143-158.
2. Calin-Jageman I & Nicholson AW (2003) Mutational analysis of an RNA internal loop as a reactivity epitope for Escherichia coli ribonuclease III substrates. Biochemistry 42: 5025-5034.
3. Court DL (1993) RNA processing and degradation by RNase III. Control of Messenger RNA Stability (Belasco JG & Brawerman G, eds), pp. 71-116. Academic Press, New York.
4. Dunn JJ (1982) Ribonuclease III. The Enzymes (Boyer PD, ed), pp. 485-499. Academic Press, New York.
5. Gan J, Shaw G, Tropea JE, Waugh DS, Court DL & Ji X (2008) A stepwise model for double-stranded RNA processing by ribonuclease III. Mol Microbiol 67: 143-154.
6. Gottesman S, McCullen CA, Guillier M, Vanderpool CK, Majdalani N, Benhammou J, Thompson KM, FitzGerald PC, Sowa NA & FitzGerald DJ (2006) Small RNA regulators and the bacterial response to stress. Cold Spring Harb Sym 71: 1-11.
7. Jaskiewicz L & Filipowicz W (2008) Role of Dicer in posttranscriptional RNA silencing. Curr Top Microbiol 320: 77-97.
8. Kim HM, Ryou SM, Song WS et al. (2009) Genetic analysis of the invariant residue G791 in Escherichia coli 16S rRNA implicates RelA in ribosome function. J Bacteriol 191: 2042-2050.
9. Lee K, Holland-Staley CA & Cunningham PR (2001) Genetic approaches to studying protein synthesis: effects of mutations at ψ516 and A535 in Escherichia coli 16S rRNA. J Nutr 131: 2994S-3004S.
10. Lee Y, Han J, Yeom KH, Jin H & Kim VN (2006) Drosha in primary microRNA processing. Cold Spring Harb Sym 71: 51-57.
11. Marles-Wright J & Lewis RJ (2007) Stress responses of bacteria. Curr Opin Struc Biol 17: 755-760.
12. Nicholson AW (1999) Function, mechanism and regulation of bacterial ribonucleases. FEMS Microbiol Rev 23: 371-390.
13. Nicholson AW (2003) The ribonuclease III superfamily: forms and functions in RNA maturation, decay, and gene silencing. RNAi: A Guide to Gene Silencing (Hannon GJ, ed), pp. 149-174. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
14. Pertzev AV & Nicholson AW (2006) Characterization of RNA sequence determinants and antideterminants of processing reactivity for a minimal substrate of Escherichia coli ribonuclease III. Nucleic Acids Res 34: 3708-3721.
15. Ramachandran V & Chen X (2008) Small RNA metabolism in Arabidopsis. Trends Plant Sci 13: 368-374.
16. Repolia F & Gottesman S (2003) Temperature sensing by the dsrA promoter. J Bacteriol 185: 6609-6614.
17. Robertson HD (1982) Escherichia coli ribonuclease III cleavage sites. Cell 30: 669-672.
18. Sim SH, Yeom JH, Shin C et al. (2010) Escherichia coli ribonuclease III activity is downregulated by osmotic stress: consequences for the degradation of bdm mRNA in biofilm formation. Mol Microbiol 75: 413-425.
19. Xiao J, Feehery CE, Tzertzinis G & Maina CV (2009) E. coli RNase III (E38A) generates discrete-sized products from long dsRNA. RNA 15: 984-991.
20. Zhang K & Nicholson AW (1997) Regulation of ribonuclease III processing by double-helical sequence antideterminants. P Natl Acad Sci USA 94: 13437-13441.