An improved chamber for direct visualisation of chemotaxis

PLoS ONE

Volumn 5, Issue 12, 2010, Pages

  Muinonen Martin, Andrew J ^a   Veltman, Douwe M ^a   Kalna, Gabriela ^a   Insall, Robert H ^a  

^a BEATSON INSTITUTE FOR CANCER RESEARCH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CANCER INVASION; CANCER RESEARCH; CELL FUNCTION; CELL MIGRATION; CELL MOTILITY; CHEMOTAXIS; CONTROLLED STUDY; DIRECT VISUALIZATION CHAMBER; GENETIC TRANSFECTION; GEOMETRY; HUMAN; HUMAN CELL; MATHEMATICAL MODEL; MELANOMA CELL; METHODOLOGY; MICROSCOPY; MICROTECHNOLOGY; CELL ADHESION; CHEMISTRY; EQUIPMENT DESIGN; MELANOMA; METABOLISM; METASTASIS; NEOPLASM; PATHOLOGY; PHASE CONTRAST MICROSCOPY; TIME; TUMOR CELL LINE;

FIBRONECTIN; FLUORESCEIN; GREEN FLUORESCENT PROTEIN;

CELL ADHESION; CELL LINE, TUMOR; CHEMOTAXIS; EQUIPMENT DESIGN; FIBRONECTINS; FLUORESCEIN; GREEN FLUORESCENT PROTEINS; HUMANS; MELANOMA; MICROSCOPY; MICROSCOPY, PHASE-CONTRAST; NEOPLASM METASTASIS; NEOPLASMS; TIME FACTORS;

EID: 78650766712     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0015309     Document Type: Article

References (26)

1. Pinner S, Sahai E (2008) PDK1 regulates cancer cell motility by antagonising inhibition of ROCK1 by RhoE. Nat Cell Biol 10: 127-137.
2. Wells A (2000) Tumor invasion: role of growth factor-induced cell motility. Adv Cancer Res 78: 31-101.
3. Insall RH (2010) Understanding eukaryotic chemotaxis: a pseudopod-centred view. Nat Rev Mol Cell Biol 11: 453-458.
4. Andrew N, Insall RH (2007) Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusions. Nat Cell Biol 9: 193-200.
5. Van Haastert PJ, Devreotes PN (2004) Chemotaxis: signalling the way forward. Nat Rev Mol Cell Biol 5: 626-634.
6. Wang W, Wyckoff JB, Frohlich VC, Oleynikov Y, Huttelmaier S, et al. (2002) Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. Cancer Res 62: 6278-6288.
7. Condeelis J, Singer RH, Segall JE (2005) The great escape: when cancer cells hijack the genes for chemotaxis and motility. Annu Rev Cell Dev Biol 21: 695-718.
8. Stathopoulos GT, Sherrill TP, Han W, Sadikot RT, Yull FE, et al. (2008) Host nuclear factor-kappaB activation potentiates lung cancer metastasis. Mol Cancer Res 6: 364-371.
9. King JS, Insall RH (2009) Chemotaxis: finding the way forward with Dictyostelium. Trends Cell Biol 19: 523-530.
10. Kedrin D, van Rheenen J, Hernandez L, Condeelis J, Segall JE (2007) Cell motility and cytoskeletal regulation in invasion and metastasis. J Mammary Gland Biol Neoplasia 12: 143-152.
11. Rhodes JM (1982) Measurement of chemotaxis in Boyden chamber filter assays. Is the checkerboard correction valid? J Immunol Methods 49: 235-236.
12. Ibarra N, Blagg SL, Vazquez F, Insall RH (2006) Nap1 regulates Dictyostelium cell motility and adhesion through SCAR-dependent and-independent pathways. Curr Biol 16: 717-722.
13. Gerisch G, Keller HU (1981) Chemotactic reorientation of granulocytes stimulated with micropipettes containing fMet-Leu-Phe. J Cell Sci 52: 1-10.
14. Li Jeon N, Baskaran H, Dertinger SK, Whitesides GM, Van de Water L, et al. (2002) Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated device. Nat Biotechnol 20: 826-830.
15. Maheshwari G, Lauffenburger DA (1998) Deconstructing (and reconstructing) cell migration. Microsc Res Tech 43: 358-368.
16. Bailly M, Yan L, Whitesides GM, Condeelis JS, Segall JE (1998) Regulation of protrusion shape and adhesion to the substratum during chemotactic responses of mammalian carcinoma cells. Exp Cell Res 241: 285-299.
17. Zigmond SH (1977) Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. J Cell Biol 75: 606-616.
18. Zicha D, Dunn GA, Brown AF (1991) A new direct-viewing chemotaxis chamber. J Cell Sci 99: 769-775.
19. Ware MF, Wells A, Lauffenburger DA (1998) Epidermal growth factor alters fibroblast migration speed and directional persistence reciprocally and in a matrix-dependent manner. J Cell Sci 111: 2423-2432.
20. Friedl P, Maaser K, Klein CE, Niggemann B, Krohne G, et al. (1997) Migration of highly aggressive MV3 melanoma cells in 3-dimensional collagen lattices results in local matrix reorganization and shedding of alpha2 and beta1 integrins and CD44. Cancer Res 57: 2061-2070.
21. Zicha D, Dunn G, Jones G (1997) Analyzing chemotaxis using the Dunn directviewing chamber. Methods Mol Biol 75: 449-457.
22. Saadi W, Wang SJ, Lin F, Jeon NL (2006) A parallel-gradient microfluidic chamber for quantitative analysis of breast cancer cell chemotaxis. Biomed Microdevices 8: 109-118.
23. Maheshwari G, Wells A, Griffith LG, Lauffenburger DA (1999) Biophysical integration of effects of epidermal growth factor and fibronectin on fibroblast migration. Biophys J 76: 2814-2823.
24. Berens P (2009) CircStat: A MATLAB Toolbox for Circular Statistics. J Stat Softw 31: 1-21.
25. van Muijen GN, Jansen KF, Cornelissen IM, Smeets DF, Beck JL, et al. (1991) Establishment and characterization of a human melanoma cell line (MV3) which is highly metastatic in nude mice. Int J Cancer 48: 85-91.
26. Breckenridge MT, Egelhoff TT, Baskaran H (2010) A microfluidic imaging chamber for the direct observation of chemotactic transmigration. Biomed Microdevices 12: 543-553.