Identification of amino acid residues responsible for increased thermostability of feruloyl esterase A from Aspergillus niger using the PoPMuSiC algorithm

Bioresource Technology

Volumn 102, Issue 2, 2011, Pages 2093-2096

  Zhang, Shuai Bing ^a,b   Wu, Zhong Liu ^a  

^a CHENGDU INSTITUTE OF BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Feruloyl esterase; PoPMuSiC; Protein engineering; Thermostability

Indexed keywords

AMINO ACID RESIDUES; AMINO ACID SUBSTITUTION; ASPERGILLUS NIGER; CATALYTIC TURNOVER; DOUBLE MUTANTS; FERULOYL ESTERASE; FOUR AMINO ACIDS; FREE ENERGY CHANGE; HALF LIVES; KEY ENZYMES; PICHIA PASTORIS; POPMUSIC; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS; SYNERGISTIC EFFECT; THERMAL STABILITY; THERMOSTABILITY; WILD-TYPE ENZYMES;

ALGORITHMS; AMINO ACIDS; BIOCHEMICAL ENGINEERING; CATALYSTS; GENETIC ENGINEERING;

ENZYMES;

FERULOYL ESTERASE A; FUNGAL ENZYME; UNCLASSIFIED DRUG;

ALGORITHM; AMINO ACID; CATALYSIS; CELLULOSE; ENZYME ACTIVITY; FUNGUS; HYDROLYSIS; PROTEIN;

ALGORITHM; AMINO ACID SUBSTITUTION; ARTICLE; ASPERGILLUS NIGER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME KINETICS; FUNGUS MUTANT; HALF LIFE TIME; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POINT MUTATION; POPMUSIC ALGORITHM; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STEADY STATE; THERMOSTABILITY; WILD TYPE;

ALGORITHMS; AMINO ACID SUBSTITUTION; AMINO ACIDS; ASPERGILLUS NIGER; CARBOXYLIC ESTER HYDROLASES; ENZYME STABILITY; KINETICS; MUTANT PROTEINS; MUTATION; PICHIA; TEMPERATURE;

ASPERGILLUS NIGER; PICHIA PASTORIS;

EID: 78650684286     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.08.019     Document Type: Article

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