Expression in Escherichia coli, refolding, and purification of the recombinant mature form of human matrix metalloproteinase 7 (MMP-7)

Bioscience, Biotechnology and Biochemistry

Volumn 74, Issue 12, 2010, Pages 2515-2517

  Muta, Yuko ^a   Yasui, Natsuki ^a   Matsumiya, Yoshiki ^b   Kubo, Motoki ^b   Inouye, Kuniyo ^a  

^a KYOTO UNIVERSITY   (Japan)

^b RITSUMEIKAN UNIVERSITY   (Japan)

Author keywords

Arginine; Escherichia coli; Matrix metalloproteinase (MMP) 7; Metalloproteinase; Refolding

Indexed keywords

ARGININE; CYSTEINE RESIDUES; DINITROPHENYL; EXPRESSION IN ESCHERICHIA COLI; HUMAN MATRIX; INCLUSION BODIES; L-ARGININE; MATRIX METALLOPROTEINASES; METALLOPROTEINASES; REFOLDING; ZINC IONS;

ESCHERICHIA COLI; PURIFICATION;

AMINO ACIDS;

ARGININE; MATRILYSIN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CELL INCLUSION; CHEMISTRY; CYTOLOGY; DRUG EFFECT; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; NUCLEOTIDE SEQUENCE; PH; PROTEIN ENGINEERING; PROTEIN REFOLDING; SOLUBILITY;

AMINO ACID SEQUENCE; ARGININE; BASE SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; HYDROGEN-ION CONCENTRATION; INCLUSION BODIES; MATRIX METALLOPROTEINASE 7; PROTEIN ENGINEERING; PROTEIN REFOLDING; RECOMBINANT PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 78650644852     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.100537     Document Type: Article

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