Improvement of transfection efficiency in cultured chicken primordial germ cells by percoll density gradient centrifugation

Bioscience, Biotechnology and Biochemistry

Volumn 74, Issue 12, 2010, Pages 2426-2430

  Oishi, Isao ^a  

^a National Institute of Advanced Industrial Science and Technology   (China)

Author keywords

Chicken PGCs; Chicken transgenesis; Percoll; Transfection

Indexed keywords

CHICK EMBRYO; CHICKEN PGCS; DENSITY GRADIENT CENTRIFUGATION; GENE TRANSFECTION; GERM CELLS; GERMLINE; IN-VIVO; MICRO-INJECTION; PERCOLL; PLASMID DNA; PLASMID TRANSFECTION; PRIMORDIAL GERM CELL; TRANSFECTANTS; TRANSFECTION; TRANSFECTION EFFICIENCY; TRANSGENESIS; TRANSIENT TRANSFECTION;

CELLS; CENTRIFUGATION; CENTRIFUGES; PURIFICATION;

ANIMALS;

DNA; PERCOLL; POVIDONE; SILICON DIOXIDE;

ANIMAL; ARTICLE; CELL CULTURE; CHICK EMBRYO; CHICKEN; CYTOLOGY; DENSITY GRADIENT CENTRIFUGATION; GENETIC TRANSFECTION; GENETICS; GERM CELL; GONAD; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; RAT; TRANSPLANTATION;

ANIMALS; CELLS, CULTURED; CENTRIFUGATION, DENSITY GRADIENT; CHICK EMBRYO; CHICKENS; DNA; GERM CELLS; GONADS; POVIDONE; RATS; SILICON DIOXIDE; TRANSFECTION;

EID: 78650640371     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.100464     Document Type: Article

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