Metabolic engineering and classic selection of the yeast Candida famata (Candida flareri) for construction of strains with enhanced riboflavin production

Metabolic Engineering

Volumn 13, Issue 1, 2011, Pages 82-88

  Dmytruk, Kostyantyn V ^a   Yatsyshyn, Valentyna Y ^a   Sybirna, Natalia O ^b   Fedorovych, Daria V ^a   Sibirny, Andriy A ^a,c  

^a INSTITUTE OF CELL BIOLOGY   (Ukraine)

^b IVAN FRANKO NATIONAL UNIVERSITY OF LVIV   (Ukraine)

^c UNIVERSITY OF RZESZÓW   (Poland)

Author keywords

Candida famata; Riboflavin; Riboflavin overproducers.; Vitamin B2; Yeast

Indexed keywords

CANDIDA; DEBARYOMYCES HANSENII; GTP CYCLOHYDROLASE; GUANOSINES; HIGH PH; HOMOLOGOUS GENES; METABOLIC ENGINEERING; METABOLIC ENGINEERING APPROACH; MYCOPHENOLIC ACIDS; NORLEUCINE; ORTHOLOGS; OVER-EXPRESSION; RANDOM MUTAGENESIS; RIBOFLAVIN; RIBOFLAVIN BIOSYNTHESIS; RIBOFLAVIN OVERPRODUCERS.; SELECTION MARKER; SHAKE-FLASK EXPERIMENTS; SYNTHETASES; TRIFLUOROMETHYL;

ACID RESISTANCE; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; GENES; METABOLISM; MUTAGENESIS; TRANSCRIPTION; TRANSCRIPTION FACTORS;

YEAST;

2 DIAZO 5 OXO LEVO NORLEUCINE; 7 METHYL 8 TRIFLUOROMETHYL 10 (1' DEXTRO RIBITYL)ISOALLOXAZINE; 8 AZAGUANINE; ANTIMETABOLITE; AZAURACIL; BIOLOGICAL MARKER; GLUCOSE; GUANOSINE; GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE II; INOSINATE DEHYDROGENASE; MYCOPHENOLIC ACID; PHENYLALANINE DERIVATIVE; RIBOFLAVIN; RIBOFLAVIN DERIVATIVE; RIBOFLAVIN SYNTHASE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR SEF1; UNCLASSIFIED DRUG;

ARTICLE; BIOMASS PRODUCTION; CANDIDA; CANDIDA FAMATA; CANDIDA FLARERI; CHEMICAL MUTAGENESIS; COLONY FORMATION; CONTROLLED STUDY; DEBARYOMYCES HANSENII; DRUG MANUFACTURE; DRUG SCREENING; DRUG STRUCTURE; DRUG SYNTHESIS; EXPERIMENTAL STUDY; FUNGAL GENE; FUNGUS CULTURE; GENE OVEREXPRESSION; GENETIC CODE; METABOLIC ENGINEERING; NONHUMAN; PH; PRIORITY JOURNAL;

CANDIDA; CLONING, MOLECULAR; FUNGAL PROTEINS; GENETIC ENHANCEMENT; RECOMBINANT PROTEINS; RIBOFLAVIN; SIGNAL TRANSDUCTION; SPECIES SPECIFICITY;

CANDIDA; DEBARYOMYCES HANSENII;

EID: 78650629843     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2010.10.005     Document Type: Article

References (41)

1. Bornemann S. Flavoenzymes that catalyse reactions with no net redox change. Nat. Prod. Rep. 2002, 19(6):761-772.
2. Burgess C.M., Smid E.J., van Sinderen D. Bacterial vitamin B2, B11 and B12 overproduction: an overview. Int. J. Food Microbiol. 2009, 133(1-2):1-7.
3. Cebollero E., Gonzalez R. Comparison of two alternative dominant selectable markers for wine yeast transformation. Appl. Environ. Microbiol. 2004, 70:7018-7023.
4. Chu S.Y., Henderson J.F. Inhibition of the phosphoribosylformylglycineamide synthetase of Ehilich ascites tumor cells by glutamine analogues. Biochem. Pharmacol. 1972, 21:401-406.
5. des Etages S.A., Falvey D.A., Reece R.J., Brandriss M.C. Functional analysis of the PUT3 transcriptional activator of the proline utilization pathway in Saccharomyces cerevisiae. Genetics 1996, 142:1069-1082.
6. Dmytruk K.V., Abbas C.A., Voronovsky A.Y., Kshanovska B.V., Sybirna K.A., Sibirny A.A. Cloning of structural genes involved in riboflavin synthesis of the yeast Candida famata. Ukr. Biokhim. Zh. 2004, 76(1):78-87.
7. Dmytruk K.V., Voronovsky A.Y., Sibirny A.A. Insertion mutagenesis of the yeast Candida famata (Debaryomyces hansenii) by random integration of linear DNA fragments. Curr. Genet. 2006, 50:183-191.
8. Exinger G., Lacroute F. 6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae. Curr. Genet. 1992, 22:9-11.
9. Fischer M., Bacher A. Biosynthesis of flavocoenzymes. Nat. Prod. Rep. 2005, 22(3):324-350.
10. Foster, E.W., Gyure, D.C., Heefner, D.L., Weaver, C.A., Yarus, M.J., Burdzinski, L.A., 1992. Riboflavin producing strains of microorganisms. US Patent 5120655.
11. Fukuda K., Watanabe M., Asano K., Ouchi K., Takasawa S. A mutated ARO4 gene for feedback-resistant DAHP synthase which causes both o-fluoro-DL-phenylalanine resistance and beta-phenethyl-alcohol overproduction in Saccharomyces cerevisiae. Curr. Genet. 1991, 20:453-456.
12. Hümbelin M., Griesser V., Keller T., Schurter W., Haiker M., Hohmann H.P., Ritz H., Richter G., Bacher A., Van Loon A.P.G.M. GTP cyclohydrolase II and 3,4-dihydroxy-2-butanone4-phosphatesynthase are rate-limiting enzymes in riboflavin synthesis of an industrial Bacillus subtilis strain used for riboflavin production. J. Ind. Microbiol. Biotechnol. 1999, 22:1-7.
13. Hyle J.W., Shaw R.J., Reines D. Functional distinctions between IMP dehydrogenase genes in providing mycophenolate resistance and guanine prototrophy to yeast. J. Biol. Chem. 2003, 278(31):28470-28478.
14. Ishchuk O.P., Dmytruk K.V., Rohulya O.V., Voronovsky A.Y., Abbas C.A., Sibirny A.A. Development of a promoter assay system for the flavinogenic yeast Candida famata based on the Kluyveromyces lactis Β-galactosidase LAC4 reporter gene. Enzyme Microb. Technol. 2008, 42:208-215.
15. Jiménez A., Santos M.A., Pompejus M., Revuelta J.L. Metabolic engineering of the purine pathway for riboflavin production in Ashbya gossypii. Appl. Environ. Microbiol. 2005, 71:5743-5751.
16. Jiménez A., Santos M.A., Revuelta J.L. Phosphoribosyl pyrophosphate synthetase activity affects growth and riboflavin production in Ashbya gossypii. BMC Biotechnol. 2008, 8:67-78.
17. Kato T., Park E.Y. Expression of alanine:glyoxylate aminotransferase gene from Saccharomyces cerevisiae in Ashbya gossypii. Appl. Microbiol. Biotechnol. 2005, 71:46-52.
18. Köhler G.A., Gong X., Bentink S., Theiss S., Pagani G.M., Agabian N., Hedstrom L. The functional basis of mycophenolic acid resistance in Candida albicans IMP dehydrogenase. J. Biol. Chem. 2005, 280:11295-11302.
19. Köhler G.A., White T.C., Agabian N. Overexpression of a cloned IMP dehydrogenase gene of Candida albicans confers resistance to the specific inhibitor mycophenolic acid. J. Bacteriol. 1997, 179:2331-2338.
20. Kuscer E., Coates N., Challis I., Gregory M., Wilkinson B., Sheridan R., Petković H. Roles of rapH and rapG in positive regulation of rapamycin biosynthesis in Streptomyces hygroscopicus. J. Bacteriol. 2007, 189:4756-4763.
21. Lim S.H., Choi J.S., Park E.Y. Microbial production of riboflavin using riboflavin overproducers, Ashbya gossypii, Bacillus subtilis, and Candida famate: an overview. Biotechnol. Bioprocess Eng. 2001, 6:75-88.
22. Lim S.H., Ming H., Park E.Y., Choi J.S. Improvement of riboflavin production using mineral support in the culture of Ashbya gossypii. Food Technol. Biotechnol. 2003, 41:137-144.
23. Massey V. The chemical and biological versatility of riboflavin. Biochem. Soc. Trans. 2000, 28(4):283-296.
24. Mateos L., Jiménez A., Revuelta J.L., Santos M.A. Purine biosynthesis, riboflavin production, and trophic-phase span are controlled by a Myb-related transcription factor in the fungus Ashbya gossypii. Appl. Environ. Microbiol. 2006, 72:5052-5060.
25. Monschau N., Sahm H., Stahmann K. Threonine aldolase overexpression plus threonine supplementation enhanced riboflavin production in Ashbya gossypii. Appl. Environ. Microbiol. 1998, 64:4283-4290.
26. Perkins, J.B., Sloma, A., Pero, J.G., Hatch, R.T., Hermann, T., Erdenberger, T., 1999. Bacterial strains which overproduce riboflavin. US Patent 5925538.
27. Sambrook J., Fritsh E.F., Maniatis T. Molecular Cloning: A Laboratory Manual 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA. second ed.
28. Schlupen C., Santos M.A., Weber U., de Graaf A., Revuelta J.L., Stahmann K.P. Disruption of the SHM2 gene, encoding one of two serine hydroxymethyltransferase isoenzymes, reduces the flux from glycine to serine in Ashbya gossypii. Biochem. J. 2003, 369:263-273.
29. Shavlovsky G.M., Logvinenko E.M., Benndorf R., Koltun L.V., Kashchenko V.E., Zakalsky A.E., Schlee D., Reinbothe H. First reaction of riboflavin biosynthesis catalysis by a guanosine triphosphate cyclohydrolase from yeast. Arch. Microbiol. 1980, 124:255-259.
30. Shi S., Chen T., Zhang Z., Chen X., Zhao X. Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production. Metab. Eng. 2009, 11:243-252.
31. Sibirny, A.A., Dmytruk, K.V., Fedorovych, D.V., 2008. Candida famata IMB Y-5034 yeast strain overproducing riboflavin (vitamin B2). UA Patent a2008 04793.
32. Stahmann K.-P., Revuelta J.L., Seulberger H. Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production. Appl. Microbiol. Biotechnol. 2000, 53:509-516.
33. Tännler S., Zamboni N., Kiraly C., Aymerich S., Sauer U. Screening of Bacillus subtilis transposon mutants with altered riboflavin production. Metab. Eng. 2008, 10:216-226.
34. Vandamme E.J. Production of vitamins, coenzymes and related biochemicals and related biotechnological processes. J. Chem. Technol. Biotechnol. 1992, 53:313-327.
35. Voronovsky A.A., Abbas C.A., Fayura L.R., Kshanovska B.V., Dmytruk K.V., Sybirna K.A., Sibirny A.A. Development of a transformation system for the flavinogenic yeast Candida famata. FEMS Yeast Res. 2002, 2:381-388.
36. Voronovsky A.Y., Abbas C.A., Dmytruk K.V., Ishchuk O.P., Kshanovska B.V., Sybirna K.A., Gaillardin C., Sibirny A.A. Candida famata (Debaryomyces hansenii) DNA sequences containing genes involved in riboflavin synthesis. Yeast 2004, 21:1307-1316.
37. Wu Q.L., Chen.T., Gan Y., Chen X., Zhao X.M. Optimization of riboflavin production by recombinant Bacillus subtilis RH44 using statistical designs. Appl. Microbiol. Biotechnol. 2007, 76:783-794.
38. Yatsyshyn V.Y., Ishchuk O.P., Voronovsky A.Y., Fedorovych D.V., Sibirny A.A. Production of flavin mononucleotide by metabolically engineered yeast Candida famata. Metab. Eng. 2009, 11:163-167.
39. Zamboni N., Maaheimo H., Szyperski T., Hohmann H.P., Sauer U. The phosphoenolpyruvate carboxykinase also catalyzes C3 carboxylation at the interface of glycolysis and the TCA cycle of Bacillus subtilis. Metab. Eng. 2004, 6:277-284.
40. Zamboni N., Mouncey N., Hohmann H.P., Sauer U. Reducing maintenance metabolism by metabolic engineering of respiration improves riboflavin production by Bacillus subtilis. Metab. Eng. 2003, 5:49-55.
41. Zhu Y.B., Chen X., Chen T., Shi S.B., Zhao X.M. Over-expression of glucose dehydrogenase improves cell growth and riboflavin production in Bacillus subtilis. Biotechnol. Lett. 2006, 28:1667-1672.