Engineering recombinant poxviruses using a compact GFP-blasticidin resistance fusion gene for selection

Journal of Virological Methods

Volumn 171, Issue 1, 2011, Pages 295-298

  Wong, Yik Chun ^a   Lin, Leon C W ^a   Melo Silva, Carolina R ^b   Smith, Stewart A ^a   Tscharke, David C ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^b Biology and Environment   (Australia)

Author keywords

Ectromelia virus; Modified Vaccinia Ankara; Poxvirus; Recombinant vaccine; Transient dominant selection; Vaccinia virus

Indexed keywords

AMINO ACID SEQUENCE; ARTICLE; BIOENGINEERING; BLASTICIDIN RESISTANCE GENE; DNA SEQUENCE; ECTROMELIA VIRUS; ENHANCED GREEN FLUORESCENT PROTEIN GENE; FUSION GENE; GENETIC CONSERVATION; HOMOLOGOUS RECOMBINATION; MARKER GENE; MODIFIED VACCINIA ANKARA VIRUS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POXVIRUS; PRIORITY JOURNAL; TRANSGENE; VACCINIA VIRUS; VIRUS RECOMBINANT; VIRUS STRAIN;

AMINOHYDROLASES; ANTIVIRAL AGENTS; DRUG RESISTANCE, VIRAL; ECTROMELIA VIRUS; GENETIC ENGINEERING; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; NUCLEOSIDES; RECOMBINANT FUSION PROTEINS; SELECTION, GENETIC; STAINING AND LABELING; VACCINIA VIRUS; VIROLOGY;

ECTROMELIA VIRUS; POXVIRIDAE; VACCINIA VIRUS;

EID: 78650567161     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.11.003     Document Type: Article

References (21)

1. Blasco R., Moss B. Selection of recombinant vaccinia viruses on the basis of plaque formation. Gene 1995, 158:157-162.
2. Boyle D.B., Coupar B.E.H. Construction of recombinant fowlpox viruses as vectors for poultry vaccines. Virus Res. 1988, 10:343-356.
3. Cao J.X., Upton C. gpt-gus fusion gene for selection and marker in recombinant poxviruses. Biotechniques 1997, 22:276-278.
4. Carroll M.W., Moss B. E. coli beta-glucuronidase (GUS) as a marker for recombinant vaccinia viruses. Biotechniques 1995, 19:352-356.
5. Chakrabarti S., Brechling K., Moss B. Vaccinia virus expression vector: coexpression of β-galactosidase provides visual screening of recombinant virus plaques. Mol. Cell. Biol. 1985, 5:3403-3409.
6. Chakrabarti S., Sisler J.R., Moss B. Compact, synthetic, vaccinia virus early/late promoter for protein expression. Biotechniques 1997, 23:1094-1097.
7. Cottingham M.G., Gilbert S.C. Rapid generation of markerless recombinant MVA vaccines by en passant recombineering of a self-excising bacterial artificial chromosome. J. Virol. Methods 2010, 168:233-236.
8. Dominguez J., Lorenzo M.M., Blasco R. Green fluorescent protein expressed by a recombinant vaccinia virus permits early detection of infected cells by flow cytometry. J. Immunol. Methods 1998, 220:115-121.
9. Falkner F.G., Moss B. Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors. J. Virol. 1988, 62:1849-1854.
10. Falkner F.G., Moss B. Transient dominant selection of recombinant vaccinia viruses. J. Virol. 1990, 64:3108-3111.
11. Franke C.A., Rice C.M., Strauss J.H., Hruby D.E. Neomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants. Mol. Cell. Biol. 1985, 5:1918-1924.
12. Hansen S.G., Cope T.A., Hruby D.E. BiZyme: a novel fusion protein-mediating selection of vaccinia virus recombinants by fluorescence and antibiotic resistance. Biotechniques 2002, 32:1178-1187.
13. Kimura M., Takatsuki A., Yamaguchi I. Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells. Biochim. Biophys. Acta 1994, 1219:653-659.
14. Lorenzo M.M., Blasco R. PCR-based method for the introduction of mutations in genes cloned and expressed in vaccinia virus. Biotechniques 1998, 24:308-313.
15. Resch W., Weisberg A.S., Moss B. Vaccinia virus nonstructural protein encoded by the A11R gene is required for formation of the virion membrane. J. Virol. 2005, 79:6598-6609.
16. Sánchez-Puig J.M., Blasco R. Puromycin resistance (pac) gene as a selectable marker in vaccinia virus. Gene 2000, 257:57-65.
17. Staib C., Drexler I., Ohlmann M., Wintersperger S., Erfle V., Sutter G. Transient host range selection for genetic engineering of modified vaccinia virus Ankara. Biotechniques 2000, 28:1137-1148.
18. Staib C., Lowel M., Erfle V., Sutter G. Improved host range selection for recombinant modified vaccinia virus Ankara. Biotechniques 2003, 34:694-696. 698, 700.
19. Tscharke D.C., Smith G.L. Notes on transient host range selection for engineering vaccinia virus strain MVA. Biotechniques 2002, 33:186-188.
20. Wyatt L.S., Earl P.L., Xiao W., Americo J.L., Cotter C.A., Vogt J., Moss B. Elucidating and minimizing the loss by recombinant vaccinia virus of human immunodeficiency virus gene expression resulting from spontaneous mutations and positive selection. J. Virol. 2009, 83:7176-7184.
21. Yang S.J., Hruby D.E. Vaccinia virus A12L protein and its AG/A proteolysis play an important role in viral morphogenic transition. Virol. J. 2007, 4:73.