Entamoeba invadens: Identification of ADF/cofilin and their expression analysis in relation to encystation and excystation

Experimental Parasitology

Volumn 127, Issue 1, 2011, Pages 195-201

  Makioka, Asao ^a   Kumagai, Masahiro ^a   Hiranuka, Kazushi ^b   Kobayashi, Seiki ^c   Takeuchi, Tsutomu ^c  

^a JIKEI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Actin cytoskeleton; ADF cofilin; Cytochalasin D; Encystation; Entamoeba invadens; Excystation; Protozoa; Real time RT PCR

Indexed keywords

ACTIN; ACTIN 1; ACTIN 2; ACTIN DEPOLYMERIZING FACTOR; COFILIN; COFILIN 1; COFILIN 2; COFILIN 3; CYTOCHALASIN D; MESSENGER RNA; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMOEBA (LIFE CYCLE STAGE); ARTICLE; CELL LYSATE; CELL MEMBRANE; CLADISTICS; CONTROLLED STUDY; CYST FORMATION; CYTOSKELETON; ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; ENTAMOEBA INVADENS; EXCYSTATION; GENE EXPRESSION; GENETIC DATABASE; GENETIC TRANSCRIPTION; IMMUNOFLUORESCENCE TEST; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE DEVELOPMENT; PHYLOGENETIC TREE; PHYLOGENY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PSEUDOPODIUM; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SPECIES DIFFERENCE; TROPHOZOITE;

ACTIN DEPOLYMERIZING FACTORS; AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; CYTOCHALASIN D; DNA, PROTOZOAN; ENTAMOEBA; GENE EXPRESSION; IMMUNE SERA; NUCLEIC ACID SYNTHESIS INHIBITORS; PHYLOGENY; POLYMERASE CHAIN REACTION; RABBITS; RNA, MESSENGER; RNA, PROTOZOAN; SEQUENCE ALIGNMENT;

ENTAMOEBA; ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; ENTAMOEBA INVADENS; METAZOA; ORYCTOLAGUS CUNICULUS; PROTOZOA; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

EID: 78650552591     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2010.07.018     Document Type: Article

References (24)

1. Bamburg J.R. Proteins of the ADF/cofilin family: essential regulators of actin dynamics. Annual Review of Cell and Developmental Biology 1999, 15:185-230.
2. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 1976, 72:248-254.
3. Cleveland L.R., Sanders E.P. Encystation, multiple fission without encystment, excystation, metacystic development, and variation in a pure line and nine strains of Entamoeba histolytica. Archives of Protistenkd 1930, 70:223-266.
4. Diamond L.S., Harlow D.R., Cunnick C.C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Transactions of the Royal Society of Tropical Medicine and Hygiene 1978, 72:431-432.
5. Dobell C. Researches on the intestinal protozoa of monkeys and man. Parasitology 1928, 20:357-412.
6. Eichinger D. Encystation of entamoeba parasites. Bioessays 1997, 19:633-639.
7. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985, 39:783-791.
8. López-Romero E., Villagómez-Castro J.C. Encystation in Entamoeba invadens. Parasitology Today 1993, 9:225-227.
9. Garcia-Zapien A.G., Hernandez-Gutierrez R., Mora-Galindo J. Simultaneous growth and mass encystation of Entamoeba invadens under axenic conditions. Archives of Medical Research 1995, 26:257-262.
10. Guan J.Q., Vorobiev S., Almo S.C., Chance M.R. Mapping the G-actin binding surface of cofilin using synchrotron protein footprinting. Biochemistry 2002, 41:5765-5775.
11. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:681-685.
12. Lappalainen P., Fedorov E.V., Fedorov A.A., Almo S.C., Drubin D.G. Essential functions and actin-binding surfaces of yeast cofilin revealed by systematic mutagenesis. EMBO Journal 1997, 16:5520-5530.
13. Maciver S.K., Hussey P.J. The ADF/cofilin family: actin-remodeling proteins. Genome Biology 2002, 3:3007.1-3007.12.
14. Makioka A., Kumagai M., Kobayashi S., Takeuchi T. Entamoeba invadens: cysteine protease inhibitors block excystation and metacystic development. Experimental Parasitology 2005, 109:27-32.
15. Makioka A., Kumagai M., Ohtomo H., Kobayashi S., Takeuchi T. Entamoeba invadens: enhancement of excystation and metacystic development by cytochalasin D. Experimental Parasitology 2001, 98:145-151.
16. McConnachie E.W. Studies on Entamoeba invadens Rodhain, 1934, in vitro, and its relationship to some other species of Entamoeba. Parasitology 1955, 45:452-481.
17. Mitra, B.N., Pradel, G., Frevert, U., Eichinger, D., 2009. Compounds of the upper gastrointestinal tract induce rapid and efficient excystation of Entamoeba invadens. International Journal for Parasitology.
18. Page R.D. TreeView: an application to display phylogenetic trees on personal computers. Computational Application of Biosciences 1996, 12:357-358.
19. Rengpien S., Bailey G.B. Differentiation of Entamoeba: a new medium and optimal conditions for axenic encystation of E. invadens. Journal of Parasitology 1975, 61:24-30.
20. Saitou N., Nei M. The neighbor-joining methods: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 1987, 4:406-425.
21. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
22. Sanchez L., Enea V., Eichinger D. Identification of a developmentally regulated transcript expressed during encystation of Entamoeba invadens. Molecular and Biochemical Parasitology 1994, 67:125-135.
23. Thompson J.D., Higgins D.G., Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 1994, 22:4673-4680.
24. Van Troys M., Dewitte D., Verschelde J.L., Goethals M., Vanderckerhove J., Ampe C. The competitive interaction of actin and PIP2 with actophorin is based on overlapping target sites: design of a gain-of-function mutant. Biochemistry 2000, 39:12181-12189.