Optimization of peptide and protein separation with a monolithic reversed-phase column and application to arsenic-binding studies

Journal of Chromatography A

Volumn 1218, Issue 2, 2011, Pages 280-285

  Schmidt, Anne Christine ^a   Mickein, Kathleen ^a  

^a TU BERGAKADEMIE FREIBERG   (Germany)

Author keywords

Arsenic binding biomolecules; Monolithic column; Peptide and protein separation; Reversed phase liquid chromatography

Indexed keywords

AMMONIUM ACETATE; APPARENT EQUILIBRIUM CONSTANT; APROTININ; BINDING STUDIES; BOX-BEHNKEN EXPERIMENTAL DESIGN; CRITICAL VARIABLES; DISULFIDE BRIDGE; ELUENT FLOW RATE; GRADIENT TIME; HPLC-UV; ION-PAIRING REAGENT; K-VALUES; MOLAR EXCESS; MOLAR RATIO; MONOLITHIC COLUMNS; MONOLITHIC REVERSED-PHASE COLUMNS; OPTIMUM VALUE; PEAK AREA; PEAK BROADENING; PROTEIN SEPARATIONS; QUANTITATIVE EVALUATION; REVERSED PHASE LIQUID-CHROMATOGRAPHY; SEPARATION FACTORS; SEPARATION METHODS; TRICHLOROACETIC ACID; UV ABSORPTION;

ACETONITRILE; AMMONIUM COMPOUNDS; ARSENIC; BIOMOLECULES; CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; EQUILIBRIUM CONSTANTS; FORMIC ACID; LIQUID CHROMATOGRAPHY; LIQUIDS; OPTIMIZATION; PEPTIDES; SULFUR;

PHASE SEPARATION;

ACETONITRILE; ALPHA LACTALBUMIN; AMMONIUM ACETATE; APROTININ; ARGIPRESTOCIN; ARSENIC; ARSENOSOBENZENE; BETA LACTOGLOBULIN; CYTOCHROME C; DISULFIDE; FORMIC ACID; LYSOZYME; OXYTOCIN; SULFUR; TRICHLOROACETIC ACID; VASOPRESSIN;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DISULFIDE BOND; ELUTION; EQUILIBRIUM CONSTANT; FLOW RATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; PH; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN BINDING; QUANTITATIVE ANALYSIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; SPECTROPHOTOMETER;

ACETONITRILES; ARSENIC; ARSENICALS; CHROMATOGRAPHY, REVERSE-PHASE; ISOELECTRIC POINT; LINEAR MODELS; PEPTIDES; PROTEINS; SENSITIVITY AND SPECIFICITY; SULFUR; TRICHLOROACETIC ACID; VASOPRESSINS; VASOTOCIN;

EID: 78650540981     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.11.031     Document Type: Article

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