A new 3D reconstituted human corneal epithelium model as an alternative method for the eye irritation test

Toxicology in Vitro

Volumn 25, Issue 1, 2011, Pages 403-410

  Jung, Kyoung Mi ^a   Lee, Su Hyon ^b   Ryu, Yang Hwan ^b   Jang, Won Hee ^a   Jung, Haeng Sun ^b   Han, Ju Hee ^c   Seok, Seung Hyeok ^c   Park, Jae Hak ^c   Son, Youngsook ^d   Park, Young Ho ^a   Lim, Kyung Min ^a  

^a AmorePacific Corporation   (South Korea)

^b Modern Cell and Tissue Technologies Inc   (South Korea)

^c Seoul National University   (South Korea)

^d Kyung Hee University   (South Korea)

Author keywords

Barrier function; Cell viability assay; Eye irritation test; MCTT HCE model; Predictive model; Protocol refinement

Indexed keywords

2 ETHYLHEXANOL; 2 HEPTANONE; 2 PROPANOL; 3 METHOXY 1,2 PROPANEDIOL; 3,3 DIMETHYLPENTANE; 4 DIMETHYLAMINOBENZOIC ACID 2 ETHYLHEXYL ESTER; 4 METHYL 2 PENTANONE; ACETONE; ALCOHOL; CD44V6 ANTIGEN; CYCLOPENTANE DERIVATIVE; CYTOKERATIN 12; CYTOKERATIN 3; GLYCEROL; IRRITANT AGENT; MACROGOL 400; METHYLCYCLOPENTANE; MUCIN 1; MUCIN 5AC; MYRISTIC ACID ISOPROPYL ESTER; OCTANOL; PENTANOL DERIVATIVE; POLYSORBATE 20; POLYSORBATE 80; PROTEIN P63; SILICIC ACID; SODIUM HYDROXIDE; TOLUENE; TRITON X 100; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; BIOLOGICAL MODEL; CELL CULTURE; CELL DIFFERENTIATION; CELL VIABILITY; CLASSIFICATION; CONTROLLED STUDY; CORNEA EPITHELIUM; CORNEA THICKNESS; ELECTRIC RESISTANCE; EYE IRRITATION; HISTOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; INCUBATION TIME; METHODOLOGY; MORPHOLOGY; PROTEIN EXPRESSION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; THREE DIMENSIONAL RECONSTRUCTED HUMAN CORNEAL EPITHELIUM MODEL; TOXICITY TESTING; TRANSEPITHELIAL ELECTRIC RESISTANCE;

3T3 CELLS; ANIMAL TESTING ALTERNATIVES; ANIMALS; BIOLOGICAL MARKERS; CELL SURVIVAL; CELLS, CULTURED; COCULTURE TECHNIQUES; ELECTRIC IMPEDANCE; EPITHELIUM, CORNEAL; HUMANS; IRRITANTS; LIMBUS CORNEAE; MICE; MODELS, BIOLOGICAL; OCTOXYNOL; REPRODUCIBILITY OF RESULTS; TIME FACTORS; TOXICITY TESTS;

EID: 78650521308     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2010.10.019     Document Type: Article

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