Accuracy of calculated free testosterone differs between equations and depends on gender and SHBG concentration

Steroids

Volumn 76, Issue 1-2, 2011, Pages 48-55

  Hackbarth, Jennifer S ^a   Hoyne, Jonathan B ^b   Grebe, Stefan K ^a   Singh, Ravinder J ^a  

^a MAYO CLINIC   (United States)

^b MAYO CLINIC   (United States)

Author keywords

Free testosterone; Free testosterone calculation; Isotope dilution equilibrium dialysis; Mass action equation; Sex hormone binding globulin; Testosterone

Indexed keywords

ALBUMIN; ISOTOPE; SEX HORMONE BINDING GLOBULIN; TESTOSTERONE;

ADOLESCENT; ADULT; AGED; ALBUMIN BLOOD LEVEL; ARTICLE; CONTROLLED STUDY; DIALYSIS; FEMALE; GOLD STANDARD; HUMAN; ISOTOPE DILUTION ASSAY; MAJOR CLINICAL STUDY; MALE; SEX DIFFERENCE; TESTOSTERONE BLOOD LEVEL;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; FEMALE; HUMANS; MALE; MIDDLE AGED; REGRESSION ANALYSIS; SEX DISTRIBUTION; SEX HORMONE-BINDING GLOBULIN; TESTOSTERONE; YOUNG ADULT;

EID: 78650513681     PISSN: 0039128X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.steroids.2010.08.008     Document Type: Article

References (39)

1. K.L. Becker 3rd ed. Principles and practice of endocrinology and metabolism xxxiv 2001 Lippincott Williams and Wilkins Philadelphia, PA 2477 pp
2. A.B. Araujo, G.R. Esche, V. Kupelian, A.B. O'Donnell, T.G. Travison, and R.E. Williams Prevalence of symptomatic androgen deficiency in men J Clin Endocrinol Metab 92 2007 4241 4247
3. A.B. Araujo, A.B. O'Donnell, D.J. Brambilla, W.B. Simpson, C. Longcope, and A.M. Matsumoto Prevalence and incidence of androgen deficiency in middle-aged and older men: estimates from the Massachusetts Male Aging Study J Clin Endocrinol Metab 89 2004 5920 5926
4. R.J. Elin, and S.J. Winters Current controversies in testosterone testing: aging and obesity Clin Lab Med 24 2004 119 139
5. J.M. Kaufman, and A. Vermeulen The decline of androgen levels in elderly men and its clinical and therapeutic implications Endocr Rev 26 2005 833 876
6. G. Bachmann, J. Bancroft, G. Braunstein, H. Burger, S. Davis, and L. Dennerstein Female androgen insufficiency: the Princeton consensus statement on definition, classification, and assessment Fertil Steril 77 2002 660 665
7. L.M. Rivera-Woll, M. Papalia, S.R. Davis, and H.G. Burger Androgen insufficiency in women: diagnostic and therapeutic implications Hum Reprod Update 10 2004 421 432
8. B.A. Mohr, S. Bhasin, C.L. Link, A.B. O'Donnell, and J.B. McKinlay The effect of changes in adiposity on testosterone levels in older men: longitudinal results from the Massachusetts Male Aging Study Eur J Endocrinol 155 2006 443 452
9. C. Gluud, M. Bahnsen, P. Bennett, U.A. Brodthagen, O. Dietrichson, and S.G. Johnsen Hypothalamic-pituitary-gonadal function in relation to liver function in men with alcoholic cirrhosis Scand J Gastroenterol 18 1983 939 944 (Pubitemid 13014820)
10. J.H. Thijssen Hormonal and nonhormonal factors affecting sex hormone-binding globulin levels in blood Ann N Y Acad Sci 538 1988 280 286
11. A.W. Meikle The interrelationships between thyroid dysfunction and hypogonadism in men and boys Thyroid 14 Suppl. 1 2004 S17 S25
12. J.E. Morley, F.E. Kaiser, H.M. Perry 3rd, P. Patrick, P.M. Morley, and P.M. Stauber Longitudinal changes in testosterone, luteinizing hormone, and follicle-stimulating hormone in healthy older men Metabolism 46 1997 410 413
13. J.F. Dunn, B.C. Nisula, and D. Rodbard Transport of steroid hormones: binding of 21 endogenous steroids to both testosterone-binding globulin and corticosteroid-binding globulin in human plasma J Clin Endocrinol Metab 53 1981 58 68
14. A. Vermeulen, T. Stoica, and L. Verdonck The apparent free testosterone concentration, an index of androgenicity J Clin Endocrinol Metab 33 1971 759 767
15. N.A. Mazer A novel spreadsheet method for calculating the free serum concentrations of testosterone, dihydrotestosterone, estradiol, estrone and cortisol: with illustrative examples from male and female populations Steroids 74 2009 512 519
16. M.L. DeVan, D.D. Bankson, and J.M. Abadie To what extent are free testosterone (FT) values reproducible between the two Washingtons, and can calculated FT be used in lieu of expensive direct measurements? Am J Clin Pathol 129 2008 459 463
17. K.K. Miller, W. Rosner, H. Lee, J. Hier, G. Sesmilo, and D. Schoenfeld Measurement of free testosterone in normal women and women with androgen deficiency: comparison of methods J Clin Endocrinol Metab 89 2004 525 533
18. E.H. Slaats, J.C. Kennedy, and H. Kruijswijk Interference of sex-hormone binding globulin in the 11Coat-A-Count” testosterone no-extraction radioimmunoassay Clin Chem 33 1987 300 302
19. K.S. Fritz, A.J. McKean, J.C. Nelson, and R.B. Wilcox Analog-based free testosterone test results linked to total testosterone concentrations, not free testosterone concentrations Clin Chem 54 2008 512 516
20. W. Rosner An extraordinarily inaccurate assay for free testosterone is still with us J Clin Endocrinol Metab 86 2001 2903
21. R. Sodergard, T. Backstrom, V. Shanbhag, and H. Carstensen Calculation of free and bound fractions of testosterone and estradiol-17 beta to human plasma proteins at body temperature J Steroid Biochem 16 1982 801 810
22. A. Vermeulen, L. Verdonck, and J.M. Kaufman A critical evaluation of simple methods for the estimation of free testosterone in serum J Clin Endocrinol Metab 84 1999 3666 3672
23. R. Lepage Measurement of testosterone and its sub-fractions in Canada Clin Biochem 39 2006 97 108
24. W. Rosner, R.J. Auchus, R. Azziz, P.M. Sluss, and H. Raff Position statement: utility, limitations, and pitfalls in measuring testosterone: an Endocrine Society position statement J Clin Endocrinol Metab 92 2007 405 413
25. W. de Ronde, Y.T. van der Schouw, H.A. Pols, L.J. Gooren, M. Muller, and D.E. Grobbee Calculation of bioavailable and free testosterone in men: a comparison of 5 published algorithms Clin Chem 52 2006 1777 1784
26. C.K. Ho, M. Stoddart, M. Walton, R.A. Anderson, and G.J. Beckett Calculated free testosterone in men: comparison of four equations and with free androgen index Ann Clin Biochem 43 2006 389 397
27. H.A. Ross, E.J. Meuleman, and F.C. Sweep A simple method for estimating equilibrium constants for serum testosterone binding resulting in an optimal free testosterone index for use in elderly men Clin Chem Lab Med 43 2005 613 616
28. H. Dechaud, A. Denuziere, S. Rinaldi, J. Bocquet, H. Lejeune, and M. Pugeat Age-associated discrepancy between measured and calculated bioavailable testosterone in men Clin Chem 53 2007 723 728
29. F. Giton, J. Fiet, J. Guechot, F. Ibrahim, F. Bronsard, and D. Chopin Serum bioavailable testosterone: assayed or calculated? Clin Chem 52 2006 474 481
30. L.P. Ly, and D.J. Handelsman Empirical estimation of free testosterone from testosterone and sex hormone-binding globulin immunoassays Eur J Endocrinol 152 2005 471 478
31. G. Sartorius, L.P. Ly, K. Sikaris, R. McLachlan, and D.J. Handelsman Predictive accuracy and sources of variability in calculated free testosterone estimates Ann Clin Biochem 46 2009 137 143
32. R.J. Singh Validation of a high throughput method for serum/plasma testosterone using liquid chromatography tandem mass spectrometry (LC-MS/MS) Steroids 73 2008 1339 1344
33. B.L. Bammann, C.B. Coulam, and N.S. Jiang Total and free testosterone during pregnancy Am J Obstet Gynecol 137 1980 293 298
34. P. Emadi-Konjin, J. Bain, and I.L. Bromberg Evaluation of an algorithm for calculation of serum “bioavailable” testosterone (BAT) Clin Biochem 36 2003 591 596
35. I. Vlahos, W. MacMahon, D. Sgoutas, W. Bowers, J. Thompson, and W. Trawick An improved ultrafiltration method for determining free testosterone in serum Clin Chem 28 1982 2286 2291
36. T. Takeuchi, O. Nishii, T. Okamura, T. Yaginuma, and T. Kawana Centrifugal ultrafiltration method for determining the percent of free testosterone in serum Nippon Naibunpi Gakkai Zasshi 63 1987 1364 1372
37. K. Van Uytfanghe, D. Stockl, J.M. Kaufman, T. Fiers, H.A. Ross, and A.P. De Leenheer Evaluation of a candidate reference measurement procedure for serum free testosterone based on ultrafiltration and isotope dilution-gas chromatography-mass spectrometry Clin Chem 50 2004 2101 2110
38. M. Jin, M.H. Wener, and D.D. Bankson Evaluation of automated sex hormone binding globulin immunoassays Clin Biochem 39 2006 91 94
39. Xita N, Tsatsoulis A. Genetic variants of sex hormone-binding globulin and their biological consequences. Mol Cell Endocrinol;316:60-5.