Purification and characterization of the ncgl2923-encoded 3-hydroxybenzoate 6-hydroxylase from Corynebacterium glutamicum

Journal of Basic Microbiology

Volumn 50, Issue 6, 2010, Pages 599-604

  Yang, Yi Fan ^a   Zhang, Jun Jie ^a   Wang, Song He ^a   Zhou, Ning Yi ^a  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

Author keywords

3 hydroxybenzoate 6 hydroxylase; Characterization; Corynebacterium glutamicum; Purification

Indexed keywords

2,5-DIHYDROXYBENZOIC ACID; 3 HYDROXYBENZOATE 6 HYDROXYLASE; 3 HYDROXYBENZOIC ACID; 3-HYDROXYBENZOATE-6-HYDROXYLASE; 3-HYDROXYBENZOIC ACID; BUFFER; FLAVINE ADENINE NUCLEOTIDE; GENTISIC ACID; HYBRID PROTEIN; HYDROXYBENZOIC ACID DERIVATIVE; MIXED FUNCTION OXIDASE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; COENZYME; CORYNEBACTERIUM GLUTAMICUM; ENZYMOLOGY; GEL CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN MULTIMERIZATION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BUFFERS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; COENZYMES; CORYNEBACTERIUM GLUTAMICUM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLAVIN-ADENINE DINUCLEOTIDE; GENTISATES; HYDROGEN-ION CONCENTRATION; HYDROXYBENZOIC ACIDS; KINETICS; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PROTEIN MULTIMERIZATION; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); CORYNEBACTERIUM GLUTAMICUM; CORYNEBACTERIUM GLUTAMICUM ATCC 13032; NEGIBACTERIA; POSIBACTERIA;

EID: 78650387382     PISSN: 0233111X     EISSN: 15214028     Source Type: Journal    
DOI: 10.1002/jobm.201000053     Document Type: Article

References (19)

1. Park, M., Jeon, Y., Jang, H.H., Ro, H.S., Park, W. et al., 2007. Molecular and biochemical characterization of 3-hydroxybenzoate 6-hydroxylase from Polaromonas naphthalenivorans CJ2. Appl. Environ. Microbiol., 73, 5146-5152.
2. Gao, X., Tan, C.L., Yeo, C.C., Poh, C.L., 2005. Molecular and biochemical characterization of the xlnD-encoded 3-hydroxybenzoate 6-hydroxylase involved in the degradation of 2, 5-xylenol via the gentisate pathway in Pseudomonas alcaligenes NCIMB 9867. J. Bacteriol., 187, 7696-7702.
3. Suarez, M., Ferrer, E., Garrido-Pertierra, A., Martin, M., 1995. Purification and characterization of the 3-hydroxybenzoate-6-hydroxylase from Klebsiella pneumoniae. FEMS Microbiol. Lett., 126, 283-290.
4. Hermann, T., 2003. Industrial production of amino acids by coryneform bacteria. J. Biotechnol., 104, 155-172.
5. Shen, X.H., Jiang, C.Y., Huang, Y., Liu, Z.P., Liu, S.J., 2005. Functional identification of novel genes involved in the glutathione-independent gentisate pathway in Corynebacterium glutamicum. Appl. Environ. Microbiol., 71, 3442-3452.
6. Hochuli, E., Dobeli, H., Schacher, A., 1987. New metal chelate adsorbent selective for proteins and peptides containing neighboring histidine-residues. J. Chromatog., 411, 177-184.
7. Hochuli, E., Bannwarth, W., Dobeli, H., Gentz, R., Stuber, D., 1988. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio-Technol., 6, 1321-1325.
8. Crowe, J., Dobeli, H., Gentz, R., Hochuli, E., Stuber, D. et al., 1994. 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification. Methods Mol. Biol., 31, 371-387.
9. Gershon, P.D., Khilko, S., 1995. Stable chelating linkage for reversible immobilization of oligohistidine tagged proteins in the biacore surface-plasmon resonance detector. J. Immunol. Methods, 183, 65-76.
10. Nieba, L., NiebaAxmann, S.E., Persson, A., Hamalainen, M., Edebratt, F. et al., 1997. BIACORE analysis of histidine-tagged proteins using a chelating NTA sensor chip. Anal. Biochem., 252, 217-228.
11. J. Sambrook, E.F.F., Maniatis, T., 1989. Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, N.Y.
12. Leatherbarrow, R.L., 2001. GraFit User's Guide. Version 5, GraFit Erithacus Software. Erithacus Software Limited, Surrey, RH6 9 YJ, UK.
13. Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254.
14. Zhou, N.Y., Al-Dulayymi, J., Baird, M.S., Williams, P.A., 2002. Salicylate 5-hydroxylase from Ralstonia sp. strain U2: a monooxygenase with close relationships to and shared electron transport proteins with naphthalene dioxygenase. J. Bacteriol., 184, 1547-1555.
15. Wierenga, R.K., Terpstra, P., Hol, W.G., 1986. Prediction of the occurrence of the ADP-binding beta alpha beta-fold in proteins, using an amino acid sequence fingerprint. J. Mol. Biol., 187, 101-107.
16. Dym, O., Eisenberg, D., 2001. Sequence-structure analysis of FAD-containing proteins. Protein Sci., 10, 1712-1728.
17. Eggink, G., Engel, H., Vriend, G., Terpstra, P., Witholt, B., 1990. Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints. J. Mol. Biol., 212, 135-142.
18. Eppink, M.H., Schreuder, H.A., Van Berkel, W.J., 1997. Identification of a novel conserved sequence motif in flavoprotein hydroxylases with a putative dual function in FAD/NAD(P)H binding. Protein Sci., 6, 2454-2458.
19. Eswaramoorthy, S., Bonanno, J.B., Burley, S.K., Swaminathan, S., 2006. Mechanism of action of a flavin-containing monooxygenase. Proc. Natl. Acad. Sci. USA, 103, 9832-9837.