Arsenate reductase activity in roots from the arsenic hyperaccumulator Pteris vittata utilizes both glutathione and dithiothreitol as reductants

Plant Biosystems

Volumn 144, Issue 4, 2010, Pages 857-859

  Nagarajan, V K ^a   Ebbs, Stephen D ^a  

^a SOUTHERN ILLINOIS UNIVERSITY   (United States)

Author keywords

Arsenate reductase; Arsenic; Hyperaccumulator; Pteris vittata

Indexed keywords

PTERIDIUM; PTERIS; PTERIS VITTATA;

EID: 78650361270     PISSN: 11263504     EISSN: 17245575     Source Type: Journal    
DOI: 10.1080/11263504.2010.522770     Document Type: Article

References (24)

1. Asher C, Reay P. 1979. Arsenic uptake by barley seedlings. Austr J Plant Physiol 6: 459-466.
2. Cao X, Ma LQ, Tu C. 2004. Antioxidative responses to arsenic in the arsenic-hyperaccumulator Chinese brake fern (Pteris vittata L.). Environ Pollut 128: 317-325.
3. Carvalho L, De Koe T, Tavares P. 1998. An improved molybdenum blue method for simultaneous determination of inorganic phosphate and arsenate. Ecotoxicol Environ Restoration 1: 13-19.
4. Duan G-L, Zhu Y-G, Tong Y-P, Cai C, Kneer R. 2005. Characterization of arsenate reductase in the extract of roots and fronds of Chinese brake fern, an arsenic hyperaccumulator. Plant Physiol 138: 461-469.
5. Ellis DR, Gumaelius L, Indriolo E, Pickering IJ, Banks JA, Salt DE. 2006. A novel arsenate reductase from the arsenic hyperaccumulating fern Pteris vittata. Plant Physiol 141: 1544-1554.
6. Francesconi K, Visoottiviseth P, Sridokchan W, Goessler W. 2002. Arsenic species in an arsenic hyperaccumulating fern, Pityrogramma calomelanos: A potential phytoremediator of arsenic-contaminated soils. Sci Total Environ 284: 27-35.
7. Gegenheimer P. 1990. Guide to protein purification. In: Deutcher MP, editor. Methods in enzymology. Vol. 182. p. 193.
8. Gregus Z, Nemeti B. 2002. Purine nucleoside phosphorylase as a cytosolic arsenate reductase. Toxicol Sci 70: 13-19.
9. Griffith O, Meister A. 1979. Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine). J Biol Chem 254: 7558-7560.
10. Johnson D, Pilson M. 1972. Spectrophotometric determination of arsenite, arsenate, and phosphate in natural waters. Analitica Chimica Acta 58: 289-299.
11. Liu J, Rosen BP. 1997. Ligand interactions of the ArsC arsenate reductase. J Biol Chem 272: 21084-21089.
12. Mukhopadhyay R, Shi J, Rosen BP. 2000. Purification and characterization of Acr2p, the Saccharomyces cerevisiae arsenate reductase. J Biol Chem 275: 21149-21157.
13. Nagarajan VK. 2005. Transport and metabolism of arsenicals in arsenic hyperaccumulating ferns [Thesis (M.S.)], Carbondale: Southern Illinois University Carbondale. 125 p. Available from: Proquest, Publication No. AAT 1432574.
14. Nagarajan VK, Ebbs SD. 2007. Transport of arsenite by the arsenic hyperaccumulating brake fern Pteris vittata is inhibited by monovalent silver. Ind J Plant Physiol 12: 312-316.
15. Nemeti B, Gregus Z. 2002. Reduction of arsenate to arsenite in hepatic cytosol. Toxicol Sci 70: 4-12.
16. Poynton CY, Huang JW, Blaylock MJ, Kochian LV, Elless MP. 2004. Mechanisms of arsenic hyperaccumulation in Pteris species: Root as influx and translocation. Planta 219: 1080-1088.
17. Rosen BP. 2002. Biochemistry of arsenic detoxification. FEBS Lett 529: 86-92.
18. Schmoger MEV, Oven M, Grill E. 2000. Detoxification of arsenic by phytochelatins in plants. Plant Physiol 122: 793-802.
19. Scott N, Hatlelid KM, MacKenzie NE, Carter DE. 1993. Reactions of arsenic(III) and arsenic(V) species with glutathione. Chem Res Toxicol 6: 102-106.
20. Srivastava M, Ma LQ, Singh N, Singh S. 2005. Antioxidant responses of hyper-accumulator and sensitive fern species to arsenic. J Exp Botany 56: 1335-1342.
21. Tu C, Ma L-Q. 2002. Effects of arsenic concentrations and forms on arsenic uptake by the hyperaccumulator ladder brake. J Environ Qual 31: 641-647.
22. Webb SM, Gaillard JF, Ma LQ, Tu C. 2003. XAS speciation of arsenic in a hyper-accumulating fern. Environ Sci Technol 37: 754-760.
23. Zhang W, Cai Y, Tu C, Ma LQ. 2002. Arsenic speciation and distribution in an arsenic hyperaccumulating plant. Sci Total Environ 300: 167-177.
24. Zhao FJ, Wang JR, Barker JHA, Schat H, Bleeker PM, McGrath SP. 2003. The role of phytochelatins in arsenic tolerance in the hyperaccumulator Pteris vittata. New Phytologist 159: 403-410.