Purification of a toxic cysteine protease produced by pathogenic Aeromonas hydrophila isolated from rainbow trout

Journal of Basic Microbiology

Volumn 50, Issue 6, 2010, Pages 538-547

  Liu, Ping Chung ^a   Chuang, Wen Hsiao ^a   Tu, Chih Ching ^a   Lee, Kuo Kau ^a  

^a NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

Author keywords

Aeromonas hydrophila; Cysteine protease; Oncorhynchus mykiss; Rainbow trout; Toxic protease

Indexed keywords

BACTERIAL TOXIN; CYSTEINE PROTEINASE; CYSTEINE PROTEINASE INHIBITOR;

AEROMONAS HYDROPHILA; ANIMAL; ANIMAL DISEASE; ARTICLE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; FISH DISEASE; GRAM NEGATIVE INFECTION; ISOLATION AND PURIFICATION; LD 50; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MICROBIOLOGY; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAINBOW TROUT; SURVIVAL;

AEROMONAS HYDROPHILA; ANIMALS; BACTERIAL TOXINS; CHROMATOGRAPHY, LIQUID; CYSTEINE PROTEASES; CYSTEINE PROTEINASE INHIBITORS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; FISH DISEASES; GRAM-NEGATIVE BACTERIAL INFECTIONS; HYDROGEN-ION CONCENTRATION; LETHAL DOSE 50; MOLECULAR WEIGHT; ONCORHYNCHUS MYKISS; SURVIVAL ANALYSIS;

AEROMONAS HYDROPHILA; BACTERIA (MICROORGANISMS); ONCORHYNCHUS MYKISS;

EID: 78650332051     PISSN: 0233111X     EISSN: 15214028     Source Type: Journal    
DOI: 10.1002/jobm.201000105     Document Type: Article

References (52)

1. Rahim, Z., Aziz, K.M.S., Huq, M.I., Saeed, H., 1985. Isolation of Aeromonas hydrophila from the wounds of five species of brackish water fish of Bangladesh. Bangladesh J. Zool. (Bangladesh), 13, 37-42.
2. Llobrea, A.T., Gacutan, R.Q., 1987. Aeromonas hydrophila associated with ulcerative disease epizootic in Laguna de Bay, Philippines. Aquaculture, 67, 273-278.
3. Cahill, M.M., 1990. Virulence factors in motile Aeromonas species. J. Appl. Bacteriol., 69, 1-16.
4. McGarey, D.J., Milanesi, L., Foley, D.P., Reyes, B. Jr. et al., 1991. The role of motile aeromonads in the fish disease: Ulcerative Disease Syndrome. Experientia, 47, 441-444.
5. Joseph, S.W., Carnahan, A., 1994. The isolation, identification, and systematics of the motile Aeromonas species. Annu. Rev. Fish Dis., 4, 315-343.
6. Dooley, J.S.G., Lallier, R., Shaw, D.H., Trust, T.J., 1985. Electrophoretic and immunochemical analyses of lipopolysaccharides from various strains of Aeromonas hydrophila. J. Bacteriol., 164, 263-269.
7. Ljungh, A., Wretlind, B., Mollby, R., 1981. Separation and characterization of enterotoxin and two hemolysins from Aeromonas hydrophila. Acta Pathol Microbiol Immunol Scand B, 89, 387-397.
8. Bernheimer, A.W., Avigad, L., 1974. Partial characterization of aerolysin, A lytic exotoxin from Aeromonas hydrophila. Infect. Immun., 9, 1016-1021.
9. Barghouthi, S., Young, R., Arceneauc, J., Olson, M., Clem, W., 1986. Novel production and purification of an Aeromonas hydrophila iron uptake siderophore (amonobactin). Proceedings of the Amercian Society of Microbiology Louisiana Chapter and Louisiana Biochemistry Society 1986 Joint Meeting Louisiana State University Medical Center. Shreveport. C1-1 (Abstract).
10. Atkinson, H., Trust, T.R., 1980. Haemagglutination properties and adherence ability of Aeromonas hydrophila. Infect. Immun., 27, 938-946.
11. Wadstrom, T., Ljungh, A., Wretlind, B., 1976. Enterotoxin, haemolysin and cytotoxic protein in Aeromonas hydrophila from human infections. Acta Pathol, Microbiol, Immunol, Scand, B, 64, 112-114.
12. Dooley, J.S.G., Lallier, R., Trust, T.J., 1986. Surface antigens of virulent strains of Aeromonas hydrophila. Vet. Immunol. Immunopathol., 12, 339-344.
13. Chabot, D.J., Thune, R.L., 1991. Proteases of the Aeromonas hydrophila complex: identification, characterization and relation to virulence in channel catfish, Ictalurus punctatus (Rafinesque). J. Fish Dis., 14, 171-183.
14. Ljungh, A., Wadstrom, T., 1982. Aeromonas toxins. Pharmacol. Ther., 15, 339-354.
15. Thune, R.L., Graham, T.E., Riddle, L.M., Amborski, R.L., 1982. Extracellular products and endotoxin from Aeromonas hydrophila: effects on age-0 channel catfish. Trans. Am. Fish. Soc., 111, 749-754.
16. Kanai, K., Wakabayashi, H., 1984. Purification and some properties of proteases from Aeromonas hydrophila. Bull. Japan. Soc. Sei. Fish., 50, 1367-1374.
17. Riddle, L., Graham, T.E., Amborski, R.L., Hugh-Jones, M., 1981. Production of haemolysin and protease by Aeromonas hydrophlia in a chemically defined medium. Dev. Biol. Stand., 49, 125-133.
18. Shotts, E.B., Hsu, T.C., Waltman, W.D., 1985. Extracellular proteolytic activity of Aeromonas hydrophlia complex. Fish Pathol., 20, 37-44.
19. Thune, R.L., Johnson, M.C., Graham, T.E., Amborski, R.L., 1986. Aeromonas hydrophila beta haemolysin: purification and examination of its role in virulence in 0-group channel catfish, Ictalurus punctatus (Rafinesque). J. Fish Dis., 9, 55-61.
20. Nord, C.E., Sjoberg, L., Wadstrom, D.T., Wretlind, B., 1975. Characterization of three Aeromonas and nine Pseudomonas species by extracellular enzymes and haemolysins. Med. Microbiol. Immunol., 161, 79-87.
21. Allan, B.J., Stevenson, R.M.W., 1981. Extracellular virulence factors of Aeromonas hydrophila in fish infections. Can. J. Microbiol., 27, 1114-1122.
22. Dahle, H.K., 1971. The purification and some properties of two Aeromonas proteinases. Acta Pathol Microbiol Immunol Scand B, 79, 726-738.
23. Amborski, R.L., Borall, R., Thune, R.L., 1984. Effects of short-term cold storage on recovery of proteases from extracellular products of Aeromonas hydrophila. Appl. Environ. Microbiol., 18, 456-458.
24. 51. J. Gen. Microbiol., 131, 1975-1979.
25. Liu, P.C., Tu, C.C., Lee, K.K., 2005. Isolation and virulence of Aeromonas hydrophila in cultured freshwater fish with skin ulcerative syndrome. FA COA. Aquaculture series No.9, Reports on Fish Disease Research, 22, 21-30.
26. Lee, K.K., Ellis, A.E., 1990. Glycerophospholipid: cholesterol acyltransferase complexed with lipopolysaccharide (LPS) is a major lethal exotoxin and cytolysin of Aeromonas salmonicida: LPS stabilizes and enhances toxicity of the enzyme. J. Bacteriol., 172, 5382-5393.
27. Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein uti-lizing the principle of protein dye binding. Anal. Biochem., 72, 248-254.
28. Hastings, T.S., Ellis, A.E., 1985. Differences in the production of haemolytic and proteolytic activities by various isolates of Aeromonas salmonicida. In: Ellis, A.E. (ed.), Fish and Shellfish Pathology. London: Academic Press, Inc. (London), Ltd., pp. 69-77.
29. 50 values. Water Air Soil Pollut, 24, 431-442.
30. Kirschke, H., Barrett, A.J., 1987. Chemistry of lysosomal proteases. In: Glaumann, H., Ballard, F.J. (eds.), Lysosomes: Their Role in Protein Breakdown. London: Academic Press, pp. 193-238.
31. Liu, P.C., Lee, K.K., Tu, C.C., Chen, S.N., 1997. Purification and characterization of a cysteine protease produced by pathogenic luminous Vibrio harveyi. Curr. Microbiol., 35, 32-39.
32. Reddy, V.Y., Zhang, Q.Y., Weiss, S.J., 1995. Pericellular mobilization of the tissue-destructive cysteine proteinases, cathepsins B. L. and S. by human monocyte-derived macrophages. Proc. Natl. Acad. Sci. USA., 92, 3849-3853.
33. Abeles, R.H., Frey, P.A., Jencks, W.P., 1992. More hydrolytic enzyme, blood clotting, and the antigen-antibody response. In: Abeles, R.H., Frey, P.A., and Jencks, W.P. (eds.) Biochemistry. Boston: Jones and Bartlett Publishers, pp. 154-159.
34. Roisen, F.J., Kirschk, H., Colella, R., Wood, L. et al., 1983. An investigation of intracellular proteinases during differentiation of cultured muscle cells. In: Katunuma, N., Umezawa H., and Holzer H. (eds.), Proteinase Inhibitors: Medical and Biological Aspects. Tokyo: Jpn. Sci. Soc. Press/Berlin: Spring-Verlag, pp. 97-109.
35. Ohtsuki, K., Taguchi, K., Sato, K., Kawabata, M., 1995. Purification of ginger proteases by DEAE-Sepharose and isoelectric focusing. Biochim. Biophys. Acta, 1243, 181-184.
36. Snijder, E.J., Wassenaar, A.L.M., Spaan, W.J.M., Gorbalenya, A.E., 1995. The arterivirus Nsp2 protease: an unusual cysteine protease with primary structure similarities to both papain-like and chymotrypsin-like protease. J. Biol. Chem., 270, 16671-16676.
37. Walker, P.A., Leong, L.E.C., Porter, A.G., 1995. Sequence and structural determinants of the interaction between the 5′-noncoding region of picornavirus RNA and rhinovirus protease 3C. J. Biol. Chem., 270, 14510-14516.
38. Pike, R., McGraw, W., Potempa, J., Travis, T., 1994. Lysine- and arginine-specific proteinases from Porphyromonas gingivalis: isolation, characterization, and evidence of complexes with hemagglutins. J. Biol. Chem., 269, 406-411.
39. Berge, A., Björck, L., 1995. Streptococcal cysteine protease releases biological active fragments of Streptococcal surface proteins. J. Biol. Chem., 270, 9862-9867.
40. Imamura, T., Potempa, J., Pike, R.N., Travis, J., 1995. Dependence of vascular premeability enhancement on cysteine proteinases in vesicles of Porphyromonas gingivalis. Infect. Immun., 63, 1999-2003.
41. Konagaya, S., 1980. Jellification and protease activity of yellowfin sole muscle in association with a Myxosporidian parasite. Bull. Japan. Soc. Sei. Fish., 46, 1019-1026.
42. Konagaya, S., 1982. Evaluation methods of the jellification grade of sporoa-infected yellowfin tuna meat, and the jellification rate. Bull. Japan. Soc. Sei. Fish., 48, 543-548.
43. Konagaya, S., 1983. Protease responsible for jellification of Myxosporidian infected swordfish meat. Bull. Japan. Soc. Sei. Fish., 49, 919-926.
44. Provenzano, D., Alderete, J.F., 1995. Analysis of human immunoglobulin-degrading cysteine proteases of Trichomonas vaginalis. Infect. Immun., 63, 3388-3395.
45. Rosenthal, P.J., McKerrow, J.H., Aikawa, M., Nagasawa, H., Leech, J.H., 1988. A malarial cysteine proteinase is necessary for hemoglobin degradation by Plasmodium falciparum. J. Clin. Invest., 82, 1560-1566.
46. Salas, F., Fichmann, J., Lee, G.K., Scott, M.D., Rosenthal, P.J., 1995. Functional expression of falcipain, a Plasmodium falciparum cysteine proteinase, support its role as a material hemoglobinase. Infect. Immun., 63, 2120-2125.
47. Stanley, S.L. Jr., Zhang, T., Rubin, D., Li, E., 1995. Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in severe combined immunodeficient mice. Infect. Immun., 63, 1587-1590.
48. Li, Q.X., Evin, G., Small, D.H., Multhaup, G. et al., 1995. Proteolytic processing of Alzheimer's disease β A4 amyloid precursor in human platelets. J. Biol. Chem., 270, 14140-14147.
49. Kamens, J., Paskind, M., Hugunin, M., Talanian, R.V. et al., 1995. Identification and characterization of ICH-2, a novel member of the interleukin-1 β-converting enzyme family of cysteine proteases. J. Biol. Chem., 270, 15250-15256.
50. Kuida, K., Lippke, J.A., Ku, G., Harding, M.W. et al., 1995. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β-converting enzyme. Science, 267, 2000-2003.
51. Kumar, S., 1995. ICE-like proteases in apoptosis. Trends Biochem. Sci., 20, 198-202.
52. Munday, N.A., Vaillancour, J.P., Ali, A., Casano, F.J. et al., 1995. Molecular cloning and pro-apoptotic activity of ICErel II and ICErel III, members of the ICE/CED-3 family of cysteine proteases. J. Biol. Chem., 270, 15870-15876.