LC-MS analysis of androgen metabolites in serum and urine from east African chimpanzees (Pan troglodytes schweinfurthii)

General and Comparative Endocrinology

Volumn 170, Issue 1, 2011, Pages 92-98

  Hauser, Barbara ^a   Mugisha, Lawrence ^b,c   Preis, Anna ^a   Deschner, Tobias ^a  

^a MAX PLANCK INSTITUTE FOR EVOLUTIONARY ANTHROPOLOGY   (Germany)

^b Ngamba Island Chimpanzee Sanctuary   (Uganda)

^c Faculty of Medicine   (Uganda)

Author keywords

Chimpanzee; Gonadal activity; Hydrolysis; LC MS; Metabolism; Serum; Testosterone; Urine

Indexed keywords

ANDROGEN; ANDROSTANEDIOL; ANDROSTANOLONE; ANDROSTERONE; EPITESTOSTERONE; ETIOCHOLANOLONE; TESTOSTERONE;

ANALYTIC METHOD; ANDROGEN BLOOD LEVEL; ARTICLE; CHIMPANZEE; CONTROLLED STUDY; FEMALE; HORMONE ACTION; HORMONE DETERMINATION; HORMONE URINE LEVEL; LIQUID CHROMATOGRAPHY; MALE; MASS SPECTROMETRY; METABOLITE; NONHUMAN; PRIORITY JOURNAL; SEX DIFFERENCE;

EID: 78650311302     PISSN: 00166480     EISSN: 10956840     Source Type: Journal    
DOI: 10.1016/j.ygcen.2010.09.012     Document Type: Article

References (38)

1. Albertson B.D., Hobson W.C., Burnett B.S., Turner P.T., Clark R.V., Schiebinger R.J., Loriaux D.L., Cutler G.B. Dissociation of cortisol and adrenal androgen secretion in the hypophysectomized, adrenocorticotropin-replaced chimpanzee. J. Clin. Endocrinol. Metab. 1984, 59:13-18.
2. Adams D.C., Anthony C.D. Using randomisation techniques to analyse behavioural data. Anim. Behav. 1996, 51:733-738.
3. Anestis S.F. Testosterone in juvenile and adolescent male chimpanzees (Pan troglodytes): effects of dominance, rank, aggression, and behavioral style. Am. J. Phys. Anthropol. 2006, 130:536-545.
4. Arlt W. Dehydroepiandrosterone and ageing. Best Pract. Res. Clin. Endocrinol. Metab. 2004, 18:363-380.
5. Carlsen, F., 2007. European studbook for the chimpanzee Pan troglodytes. Copenhagen Zoo, Roskildevej 38, PO Box 7, DK-2000 Frederiksberg, Denmark.
6. Ceglarek U., Kortz L., Leichtle A., Fiedler G.M., Kratzsch J., Thiery J. Rapid quantification of steroid patterns in human serum by on-line solid phase extraction combined with liquid chromatography-triple quadrupole linear ion trap mass spectrometry. Clin. Chim. Acta 2009, 401:114-118.
7. Conley A.J., Pattison J.C., Bird I.M. Variations in adrenal androgen production among (nonhuman) primates. Sem. Reproduc. Med. 2004, 22:311-326.
8. Copeland K.C., Eichberg J.W., Parker R., Bartke A. Puberty in the chimpanzee: somatomedin-C and its relationship to somatic growth and steroid hormone concentrations. J. Clin. Endocrinol. Metabol. 1985, 60:1154-1160.
9. Cutler G.B., Glenn M., Bush M., Hodgen G.D., Graham C.E., Loriaux D.L. Adrenarche: a survey of rodents, domestic animals, and primates. Endocrinology 1978, 103:2112-2118.
10. Dehennin L., Matsumoto A.M. Long-term administration of testosterone enanthate to normal men: alterations of the urinary profile of androgen metabolites potentially useful for detection of testosterone misuse in sport. J. Steroid Biochem. Molec. Biol. 1993, 44:179-189.
11. Dittami J., Katina S., Möstl E., Eriksson J., Machatschke I.H., Hohmann G. Urinary androgens and cortisol metabolites in field-samples bonobos (Pan pansicus). Gen. Comp. Endocrinol. 2008, 155:552-557.
12. Dixson A.F. Primate Sexuality 1998, Oxford University Press, Oxford.
13. Donike M., Ueki M., Kuroda Y., Geyer H., Nolteernsting E., Rauth S., Schänzer W., Schindler U., Völker E., Fujisake M. Detection of dihydrotestosterone (DHT) doping: alterations in the steroid profile and reference ranges for DHT and its 5α-metabolites. J. Sports Med. Phys. Fitness 1995, 35:235-250.
14. Felig P., Baxter J.D., Frohmann L.A. Endocrinology and Metabolism 1995, McGraw-Hill, Inc., New York. third ed.
15. Geyer H., Mareck-Engelke U., Schänzer W., Donike M. The Cologne protocol to follow up high testosterone/epitestosterone ratios. Recent Advances in Doping Analysis (4) 1997, 107-125. Sport und Buch Strauß, Köln. W. Schänzer, H. Geyer, A. Gotzmann, U. Mareck-Engelke (Eds.).
16. Haccou P., Meelis E. Statistical Analyses of Behavioural Data 1994, Oxford University Press, Oxford.
17. Hagey L.R., Czekala N.M. Comparative urinary androstanes in the great apes. Gen. Comp. Endocrinol. 2003, 130:64-69.
18. Hauser B., Deschner T., Boesch C. Development of a liquid chromatography-tandem mass spectrometry method for the determination of 23 endogenous steroids in small quantities of primate urine. J. Chromatogr. B 2008, 862:100-112.
19. Hauser B., Schulz D., Bosch C., Deschner T. Measuring urinary testosterone values of the great apes-problems with enzymatic hydrolysis using Helix pomatia juice. Gen. Comp. Endocrinol. 2008, 158:77-86.
20. Hunter M.A., May R.B. Some myths concerning parametric and nonparametric-tests. Can. Psychol. 1993, 34:384-389.
21. Klinkova E., Heistermann M., Hodges J.K. Social parameters and urinary testosterone level in male chimpanzees (Pan troglodytes). Horm. Behav. 2004, 46:474-481.
22. Lapik O., Hampl R., Hill M., Stárka L. Plasma levels of epitestosterone from prepuberty to adult life. J. Steroid Biochem. Molec. Biol. 1995, 55:405-408.
23. Manly B.F.J. Randomization, Bootstrap and Monte Carlo Methods in Biology 1997, Chapman & Hall, New York.
24. Michael G. Biochemical Pathways. Spektrum Akadem 1999, Heidelberg, Verlag.
25. Möhle U., Heistermann M., Palme R., Hodges J.K. Characterization of urinary and fecal metabolites of testosterone and their measurement for assessing gonadal endocrine function in male nonhuman primates. Gen. Comp. Endocrinol. 2002, 129:135-145.
26. Muller M.N., Wrangham R.W. Dominance, aggression and testosterone in wild chimpanzees: a test of the 'challenge hypothesis'. Anim. Behav. 2004, 67:113-123.
27. Mundry R., Fischer J. Use of statistical programs for nonparametric tests of small samples often leads to incorrect P-values: examples from Animal Behaviour. Anim. Behav. 1998, 56:256-259.
28. Palonek E., Gottlieb C., Garle M., Björkhelm I., Carlström K. Serum and urinary markers of exogenous testosterone administration. J. Steroid Biochem. Molec. Biol. 1995, 55:121-127.
29. Potter D.M., Griffiths D.J. Omnibus permutation tests of the overall null hypothesis in datasets with many covariates. J. Biopharm. Stat. 2006, 16:327-341.
30. Rauh M., Gröschl M., Rascher W., Dörr H.G. Automated, fast and sensitive quantification of 17α-hydroxy-progesterone, androstenedione and testosterone by tandem mass spectrometry with on-line extraction. Steroids 2006, 71:450-458.
31. Shackleton C.H.L., Roitman E., Phillips A., Chang T. Androstanediol and 5-androstenediol profiling for detecting exogenously administered dihydrotestosterone, epitestosterone, and dehydroepiandrosterone: potential use in gas chromatography isotope ratio mass spectrometry. Steroids 1997, 62:665.
32. Sannen A., Heistermann M., Van Elsacker L., Möhle U., Eens M. Urinary testosterone metabolite levels in bonobos: a comparison with chimpanzees in relation to social system. Behaviour 2003, 140:683-696.
33. Siegel S., Castellan N.J. Nonparametric statistics for the behavioural sciences 1988, Second ed. McGraw-Hill, New York.
34. Smail P.J., Faiman C., Hobson W.C., Fuller G.B., Winter J.S.D. Further studies on adrenarche in nonhuman primates. Endocrinology 1982, 111:844-848.
35. Stárka L. Epitestosterone. J. Steroid Biochem. Molec. Biol. 2003, 87:27-34.
36. Thienpont L.M., Van Uytfanghe K., Blincko S., Ramsay C.S., Xie H., Doss R.C. State-of the-art of serum testosterone measurement by isotope dilution-liquid chromatography tandem mass spectrometry. Clin. Chem. 2008, 54:1290-1297.
37. Wingfield J.C., Hegner R.E., Dufty A.M., Ball G.F. The 'challenge hypothesis': theoretical implications for patterns of testosterone secretion, mating systems, and breeding strategies. Am. Nat. 1990, 136:829-846.
38. Wudy S.A., Hartmann M.F. Gas chromatography-mass spectrometry profiling of steroids in times of molecular biology. Horm. Metab. Res. 2004, 36:415-422.