Tandem affinity purification combined with inducible shRNA expression as a tool to study the maturation of macromolecular assemblies

RNA

Volumn 17, Issue 1, 2011, Pages 189-200

  Wyler, Emanuel ^a,b   Zimmermann, Mirjam ^a   Widmann, Barbara ^a,b   Gstaiger, Matthias ^a   Pfannstiel, Jens ^c   Kutay, Ulrike ^a   Zemp, Ivo ^a  

^a ETH ZURICH   (Switzerland)

^b Molecular Life Science PhD Program   (Switzerland)

^c UNIVERSITY OF HOHENHEIM   (Germany)

Author keywords

40S subunit; C21orf70; Ribosome biogenesis; Rio2 kinase; Rrp12; Tandem affinity purification

Indexed keywords

SHORT HAIRPIN RNA;

ARTICLE; BINDING AFFINITY; BIOGENESIS; CELL LOSS; CELL MATURATION; EMBRYO; HUMAN; HUMAN CELL; MACROMOLECULE; MATURATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DEPLETION; PROTEIN EXPRESSION; RIBOSOME; RNA PURIFICATION; SOMATIC CELL; TANDEM AFFINITY PURIFICATION;

BLOTTING, NORTHERN; CELLS, CULTURED; CHROMATOGRAPHY, AFFINITY; FLUORESCENT ANTIBODY TECHNIQUE; HELA CELLS; HUMANS; IMMUNOPRECIPITATION; KIDNEY; MULTIPROTEIN COMPLEXES; PROTEIN-SERINE-THREONINE KINASES; RIBOSOME SUBUNITS, SMALL, EUKARYOTIC; RNA, SMALL INTERFERING; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 78650286656     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.2325911     Document Type: Article

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