Purification of rabbit polyclonal immunoglobulin G using anion exchangers

Process Biochemistry

Volumn 46, Issue 1, 2011, Pages 101-107

  Wongchuphan, Rattana ^a,b   Tey, Beng Ti ^a   Tan, Wen Siang ^a   Subramanian, Senthil Kumar ^a   Taip, Farah Saleena ^a   Ling, Tau Chuan ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b SURATTHANI RAJABHAT UNIVERSITY   (Thailand)

Author keywords

Albumin removal; Anion exchange adsorbents; Negative chromatography antibody purification; Polyclonal IgG; Rabbit serum; STREAMLINE DEAE

Indexed keywords

ALBUMIN REMOVAL; ANION-EXCHANGE ADSORBENT; NEGATIVE CHROMATOGRAPHY ANTIBODY PURIFICATION; POLYCLONAL IGG; RABBIT SERUM;

ADSORPTION; BODY FLUIDS; CHROMATOGRAPHY; ION EXCHANGE RESINS; IONS; OPTIMIZATION; PH EFFECTS; PURIFICATION;

ANTIBODIES;

ORYCTOLAGUS CUNICULUS;

EID: 78650229989     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2010.07.023     Document Type: Article

References (31)

1. D. Low, R. O'Leary, and N.S. Pujar Future of antibody purification J Chromatogr B 848 2007 48 63
2. S. Hober, K. Nord, and M. Linhult Protein A chromatography for antibody purification J Chromatogr B 848 2007 40 47
3. L. Giovannoni, M. Ventani, and U. Gottschalk Antibody purification using membrane adsorbers BioPharm Int 21 2008 48 52
4. B.C.C. Pessela, R. Munilla, L. Betancor, M. Fuentes, A.V. Carrascosa, and A. Vian Ion exchange using poorly activated supports, and easy way for purification of large proteins J Chromatogr A 1034 2004 155 159
5. S.C. Goheen, and J.L. Hilsenbeck High-performance ion-exchange chromatography and adsorption of plasma proteins J Chromatogr A 816 1998 89 96
6. D.-Q. Lin, H.M. Fernández-Lahore, M.-R. Kula, and J. Thömmes Minimising biomass/support interactions in expanded bed adsorption processes: a methodological design approach Bioseparation 10 2001 7 19
7. Y. Qi, Z. Yan, and J. Huang Chromatography on DEAE ion-exchange and Protein G affinity columns in tandem for the separation and purification of proteins J Biochem Biophys Methods 49 2001 263 273
8. Y. Huang, L. Bi, W. Zhou, Y. Li, Y. Wang, and G. Ma Improving recovery of recombinant hepatitis B virus surface antigen by ion-exchange chromatographic supports with low ligand density Process Biochem 41 2006 2320 2326
9. B.C.C. Pessela, R. Torres, P. Batalla, M. Fuentes, C. Mateo, and R. Fernández-Lafuente Simple purification of immunoglobulins from whey proteins concentrate Biotechnol Prog 22 2006 590 594
10. H.A. Chase, B. Machielse, and D. Naveh Characterization of the adsorption of immunoglobulin M onto Q Sepharose Fast Flow ion exchangers Bioseparation 7 1997 47 55
11. M. Fuentes, B.C.C. Pessela, C. Mateo, R. Munilla, J.M. Guisán, and R. Fernández-Lafuente Detection and purification of two antibody-antigen complexes via selective adsorption on lowly activated anion exchangers J Chromatogr A 1059 2004 89 94
12. C.J. Slade Molecular (or bio-) imprinting of bovine serum albumin J Mol Catal B: Enzym 9 2000 97 105
13. V. Militello, V. Verti, and M. Leone Conformational changes involved in thermal aggregation processes of bovine serum albumin Biophys Chem 105 2003 133 141
14. S.M. Vaiana, A. Emanuele, M.B. Palma-Vittorelli, and M.U. Palma Irreversible formation of intermediate BSA oligomers requires and induces conformational changes Proteins: Struct Funct Bioinf 55 2004 1053 1062
15. M. Fuentes, B.C.C. Pessela, C. Mateo, J.M. Palomo, P. Batalla, and R. Fernández-Lafuente Adsorption behavior of bovine serum albumin on lowly activated anionic exchangers suggests a new strategy for solid-phase proteomics Biomacromolecules 7 2006 1357 1361
16. W.S. Tan, M.R. Dyson, and K. Murray Hepatitis B virus core antigen: enhancement of its production in Escherichia coli, and interaction of the core particles with the viral surface antigen Biol Chem 384 2003 363 371
17. M.R. Dyson, and K. Murray Selection of peptide inhibitors of interactions involved in complex protein assemblies: association of the core and surface antigens of hepatitis B virus Proc Natl Acad Sci USA 92 1995 2194 2198
18. W.S. Tan, M.R. Dyson, and K. Murray Two distinct segments of the hepatitis B virus surface antigen contribute synergistically to its association with the viral core particles J Mol Biol 286 1999 797 808
19. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding Anal Biochem 72 1976 248 254
20. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
21. B.T. Tey, K.H. Yong, H.P. Ong, T.C. Ling, S.T. Ong, and Y.P. Tan Optimal conditions for hepatitis B core antigen production in shaked flask fermentation Biotechnol Bioprocess Eng 9 2004 374 378
22. M.Y.T. Ng, W.S. Tan, N. Abdullah, T.C. Ling, and B.T. Tey Direct purification of recombinant hepatitis B core antigen from two different pre-conditioned unclarified Escherichia coli feedstocks via expanded bed adsorption chromatography J Chromatogr A 1172 2007 47 56
23. E. Engvall, and P. Perlmann Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G Immunochemistry 8 1971 871 874
24. C. Mateo, O. Abian, R. Fernández-Lafuente, and J.M. Guisán Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support-polyethylenimine composites Biotechnol Bioeng 68 2000 98 105
25. B.C.C. Pessela, R. Fernández-Lafuente, M. Fuentes, A. Vian, J.L. Garcia, and A.V. Carrascosa Reversible immobilization of a thermophilic β-galactosidase via ionic adsorption on PEI-coated Sepabeads Enzyme Microb Technol 32 2003 369 374
26. M. Fuentes, C. Mateo, B.C.C. Pessela, J.M. Guisán, and R. Fernández-Lafuente Purification, stabilization and concentration of very weak protein-protein complexes: shifting the association equilibrium via complex selective adsorption on lowly activated supports Proteomics 5 2005 4062 4069
27. C.R. Middaugh, and D.B. Volkin Protein solubility T.J. Ahern, M.C. Manning, Stability of protein pharmaceuticals. Part A: chemical and physical pathways of protein degradation. Pharmaceutical biotechnology vol. 2 1992 Plenum Press New York 109 134
28. E.Y. Chi, S. Krishnan, T.W. Randolph, and J.F. Carpenter Physical stability of proteins in aqueous solution: mechanism and driving force in nonnative protein aggregation Pharm Res 20 2003 1325 1336
29. P. Bagchi, and S.M. Birnbaum Effect of pH on the adsorption of immunoglobulin G on anionic poly(vinyltoluene) model latex particles J Colloid Interface Sci 83 1981 460 478
30. D. Bulone, V. Martorana, and P.L. San Biagio Effects of intermediates on aggregation of native bovine serum albumin Biophys Chem 91 2001 61 69
31. G.M. Jordan, S. Yoshida, and T. Terao The aggregation of bovine serum albumin in solution and in the solid state J Pharm Pharmacol 46 1994 182 185