Modeling shear-induced CHO cell damage in a rotary positive displacement pump

Biotechnology Progress

Volumn 26, Issue 6, 2010, Pages 1606-1615

  Kamaraju, Hari ^a   Wetzel, Kenneth ^a   Kelly, William J ^a  

^a Villanova University   (United States)

Author keywords

Cell death; CHO cells; Modeling; PD pump; Shear

Indexed keywords

ANIMAL CELLS; BACK PRESSURES; BIOTECHNOLOGY INDUSTRY; CELL CONCENTRATIONS; CHO CELL; COMMONLY USED; COMPLEX FLOW; CROSS FLOW FILTRATION; MATHEMATICAL MODELING; MODELING; OPERATING CONDITION; PD PUMP; POSITIVE DISPLACEMENT PUMPS; PRODUCT YIELDS; PUMP GEOMETRY; ROTARY LOBE PUMPS; SHEAR; SHEAR DAMAGE; SHEAR RATES; SINGLE PASS; SLIP FLOW;

CELL DEATH; POPULATION STATISTICS; SHEAR DEFORMATION;

PUMPS;

ANIMAL; ARTICLE; BIOLOGICAL MODEL; BIOREACTOR; BIOTECHNOLOGY; CELL CULTURE; CELL DEATH; CELL SURVIVAL; CHO CELL; CRICETULUS; HAMSTER; INSTRUMENTATION; METHODOLOGY;

ANIMALS; BIOREACTORS; BIOTECHNOLOGY; CELL DEATH; CELL SURVIVAL; CELLS, CULTURED; CHO CELLS; CRICETINAE; CRICETULUS; MODELS, BIOLOGICAL;

ANIMALIA;

EID: 78650188057     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.479     Document Type: Article

References (20)

1. Vickroy B, Lorenz K, Kelly W. Modeling shear damage to suspended CHO cells during cross-flow filtration. Biotechnol Prog. 2007; 23: 194-199
2. Mollet M, Ma N, Zhau Y, Brodkey R, Taticek R, Chalmers J. Bioprocess equipment: characterization of energy dissipation rate and its potential to damage cells. Biotechnol Prog. 2004; 20: 1437-1448.
3. McQueen A, Bailey J. Influence of serum level, cell line, flow type and viscosity on flow-induced lysis of suspended animal cells. Biotecholnol Lett. 1989; 11: 531-536.
4. Thomas CR. Review: shear effects on cells in bioreactors. Process Sol-Liq Suspensions. 1993; 158-191.
5. Mardikar SH, Niranjan K.. Observations on the shear damage to different animal cells in a concentric viscometer. Biotechnol Bioeng. 2000; 68: 697-704.
6. Pol L, Tramper J. Shear sensitivity of animal cells from a culture-medium perspective. Trends Biotechnol. 1998; 16: 323-328.
7. Keane JT, Ryan D, Gray PP. Effect of shear stress on expression of a recombinant protein by Chinese hamster ovary cells. Biotechnol Bioeng. 2003; 81: 211-220.
8. Ludwig A, Tomeczkowski J, Kretzmer G. Influence of the temperature on the shear stress sensitivity of adherent BHK 21 cells. Appl Microbiol Biotechnol. 1992; 38: 323-327.
9. Maiorella B, and Harano D. Cross flow microfiltration of animal cells. Biotechnol Bioeng. 1991; 37: 121-126.
10. Ma N, Chalmers J. Fabrication and use of transient contractional flow device to quantify shear sensitivity of mammalian and insect to hydrodynamic forces. Biotechnol Bioeng. 2002; 80: 428-437.
11. Vandanjon L, Rossignol N, Jaouen P, Robert JM, Quemeneur F. Effects of shear on two microalgae species. Contribution of pumps and valves in tangential flow filtration systems. Biotechnol Bioeng. 1999; 63: 1-9.
12. Alias CB, Lopez MCG, Fernandez FGA, Sevilla JMF, Sanchez JLG, Grima EM. Influence of power supply in the feasibility of phaeodactylum tricornutum cultures. Biotechnol Bioeng. 2004; 87: 23-733.
13. Jaouen P, Vandanjon L, Quemeneur F. The shear of microalgal cell suspensions (Tetraselmis suecica) in tangential flow filtration systems: the role of pumps. Bioresour Technol. 1991; 68: 149-154.
14. Prakash M, Stokes N, Bertolini J, Tatford O, Gomme P. SPH Simulations of a lobe pump: prediction of protein shear stress at different pump efficiencies. Third International Conference on CFD in the Minerals and Process Industries. Melbourne, Australia; 2003.
15. Gomme P, Prakash M, Cleary P, Bertolini J. Effect of lobe pumping on human albumin: development of a lobe pump simulator using smooth particle hydrodynamics. Biotechnol Appl Biochem. 2006; 43: 113-120
16. Voorde JV, Vierendeels J, Dick E. A force- based grid manipulator for ALE calculations in a lobe pump. J Therm Sci. 2003; 12: 318-322.
17. Voorde JV, Vierendeels J, Dick E. Flow simulations in rotary volumetric pumps and compressors with the fictitious domain method. J Comput Appl Math. 2004; 168: 491-499.
18. Gomme P, Hunt B, Johnston A, Bertolini J. Effect of lobe pumping on human albumin: investigating the underlying mechanisms of aggregate formation. Biotechnol Appl Biochem. 2006; 43: 103-111
19. Carlson A, Jem K. Mechanical disruption of Escherichia coli for plasmid recovery. Biotechnol Bioeng. 1995; 48: 303-315
20. Pol L, Tramper J. Shear sensitivity of animal cells from a culture-medium perspective. Tibtech 1998; 16: 323-328.