gyrB multiplex PCR to differentiate between Acinetobacter calcoaceticus and Acinetobacter genomic species 3

Journal of Clinical Microbiology

Volumn 48, Issue 12, 2010, Pages 4592-4594

  Higgins, Paul G ^a   Lehmann, Marlene ^a   Wisplinghoff, Hilmar ^a   Seifert, Harald ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACINETOBACTER; ACINETOBACTER BAUMANNII; ACINETOBACTER CALCOACETICUS; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; GENE AMPLIFICATION; GYRB GENE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS;

ACINETOBACTER; ACINETOBACTER INFECTIONS; BACTERIOLOGICAL TECHNIQUES; DNA GYRASE; DNA PRIMERS; ELECTROPHORESIS, AGAR GEL; HUMANS; POLYMERASE CHAIN REACTION;

ACINETOBACTER; ACINETOBACTER BAUMANNII; ACINETOBACTER CALCOACETICUS;

EID: 78650063742     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01765-10     Document Type: Article

References (14)

1. Bernards, A. T., J. van der Toorn, C. P. A. van Boven, and L. Dijkshoorn. 1996. Evaluation of the ability of a commercial system to identify Acinetobacter genomic species. Eur. J. Clin. Microbiol. Infect. Dis. 15:303-308.
2. Bouvet, P. J. M., and P. A. D. Grimont. 1986. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp-nov, Acinetobacter haemolyticus sp-nov, Acinetobacter johnsonii sp-nov, and Acinetobacter junii sp-nov and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. Int. J. Syst. Bacteriol. 36:228-240.
3. Bouvet, P. J. M., and P. A. D. Grimont. 1987. Identification and biotyping of clinical isolates of Acinetobacter. Ann. Inst. Pasteur/Microbiol. 138:569-578.
4. Chang, H. C., Y. F. Wei, L. Dijkshoorn, M. Vaneechoutte, C. T. Tang, and T. C. Chang. 2005. Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region. J. Clin. Microbiol. 43:1632-1639.
5. Dijkshoorn, L., A. Nemec, and H. Seifert. 2007. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat. Rev. Microbiol. 5:939-951.
6. Ehrenstein, B., A. T. Bernards, L. Dijkshoorn, P. Gerner Smidt, K. J. Towner, P. J. M. Bouvet, F. D. Daschner, and H. Grundmann. 1996. Acinetobacter species identification by using tRNA spacer fingerprinting. J. Clin. Microbiol. 34:2414-2420.
7. Gerner-Smidt, P., I. Tjernberg, and J. Ursing. 1991. Reliability of phenotypic tests for identification of Acinetobacter species. J. Clin. Microbiol. 29:277-282.
8. Higgins, P. G., H. Wisplinghoff, O. Krut, and H. Seifert. 2007. A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU. Clin. Microbiol. Infect. 13:1199-1201.
9. Horrevorts, A., K. Bergman, L. Kollee, I. Breuker, I. Tjernberg, and L. Dijkshoorn. 1995. Clinical and epidemiologic investigations of Acinetobacter genomospecies-3 in a neonatal intensive-care unit. J. Clin. Microbiol. 33:1567-1572.
10. La Scola, B., V. A. K. B. Gundi, A. Khamis, and D. Raoult. 2006. Sequencing of the rpoB gene and flanking spacers for molecular identification of Acinetobacter species. J. Clin. Microbiol. 44:827-832.
11. Seifert, H., L. Dijkshoorn, P. Gerner Smidt, N. Pelzer, I. Tjernberg, and M. Vaneechoutte. 1997. Distribution of Acinetobacter species on human skin: comparison of phenotypic and genotypic identification methods. J. Clin. Microbiol. 35:2819-2825.
12. Seifert, H., and P. Gerner Smidt. 1995. Comparison of ribotyping and pulsed-field gel-electrophoresis for molecular typing of Acinetobacter isolates. J. Clin. Microbiol. 33:1402-1407.
13. Vaneechoutte, M., L. Dijkshoorn, I. Tjernberg, A. Elaichouni, P. Devos, G. Claeys, and G. Verschraegen. 1995. Identification of Acinetobacter genomic species by amplified ribosomal DNA restriction analysis. J. Clin. Microbiol. 33:11-15.
14. Wisplinghoff, H., M. B. Edmond, M. A. Pfaller, R. N. Jones, R. P. Wenzel, and H. Seifert. 2000. Nosocomial bloodstream infections caused by Acinetobacter species in United States hospitals: clinical features, molecular epidemiology, and antimicrobial susceptibility. Clin. Infect. Dis. 31:690-697.