Small-Interfering RNA-Mediated Identification and Regulation of the Ternary SNARE Complex Mediating RBL-2H3 Mast Cell Degranulation

Scandinavian Journal of Immunology

Volumn 73, Issue 1, 2011, Pages 8-17

  Woska, J R ^a   Gillespie, M E ^a  

^a ST JOHN'S UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FC RECEPTOR; MEMBRANE PROTEIN; SMALL INTERFERING RNA; SNARE PROTEIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 23; SYNTAXIN 4; UNCLASSIFIED DRUG; VESICLE ASSOCIATED MEMBRANE PROTEIN 7; VESICLE ASSOCIATED MEMBRANE PROTEIN 8;

ARTICLE; DOWN REGULATION; HUMAN; HUMAN CELL; MAST CELL DEGRANULATION; PRIORITY JOURNAL; RNA INTERFERENCE;

CELL DEGRANULATION; CELL LINE; DOWN-REGULATION; HUMANS; IMMUNOBLOTTING; MAST CELLS; QA-SNARE PROTEINS; QB-SNARE PROTEINS; QC-SNARE PROTEINS; R-SNARE PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, SMALL INTERFERING; SNARE PROTEINS;

EID: 78650061529     PISSN: 03009475     EISSN: 13653083     Source Type: Journal    
DOI: 10.1111/j.1365-3083.2010.02471.x     Document Type: Article

References (33)

1. Abraham SN, Malaviya R. Mast cells in infection and immunity. Infect Immun 1997;65:3501-8.
2. Kumar V, Sharma A. Mast cells: emerging sentinel innate immune cells with diverse role in immunity. Mol Immunol 2010; doi:
3. Rao KN, Brown MA. Mast cells: multifaceted immune cells with diverse roles in health and disease. Ann NY Acad Sci 2008;1143:83-104.
4. Galli SJ, Nakae S, Tsai M. Mast cells in the development of adaptive immune responses. Nat Immun 2005;6:135-42.
5. Jahn R. Principles of exocytosis and membrane fusion. Ann NY Acad Sci 2004;1014:170-8.
6. Jahn R, Scheller RH. SNAREs- engines for membrane fusion. Nat Rev 2006;7:631-43.
7. Leabu M. Membrane fusion in cells: molecular machinery and mechanisms. J Cell Mol Med 2006;10:423-7.
8. Logan MR, Odemuyiwa SO, Moqbel R. Understanding exocytosis in immune and inflammatory cells: the molecular basis of mediator secretion. J Allergy Clin Immunol 2003;111:923-32.
9. Stow JL, Manderson AP, Murray RZ. SNAREing immunity: the role of SNAREs in the immune system. Nat Rev 2006;6:919-29.
10. Rizo J, Chen X, Arac D. Unraveling the mechanisms of synaptotagmin and SNARE function in neurotransmitter release. Trends Cell Biol 2006;16:339-50.
11. Hibi T, Hirashima N, Nakanishi M. Rat basophilic leukemia cells express Syntaxin-3 and VAMP-7 in granule membranes. Biochem Biophys Res Commun 2000;271:36-41.
12. Paumet F, Mao JL, Martin S et al. Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment. J Immunol 2000;164:5850-7.
13. Guo Z, Turner C, Castle D. Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells. Cell 1998;94:537-48.
14. Pombo I, Rivera J, Blank U. Munc18-2/syntaxin3 complexes are spatially separated from syntaxin3-containing SNARE complexes. FEBS Lett 2003;550:144-8.
15. Salinas E, Ventura J, Cordova LE, Quintanar JL. Presence of SNAP-25 in rat mast cells. Immunol Lett 2004;95:105-8.
16. Hepp R, Puri N, Hohenstein AC, Crawford GL, Whiteheart SW, Roche PA. Phosphorylation of SNAP-23 regulates exocytosis from mast cells. J Biol Chem 2005;280:6610-20.
17. Lippert U, Ferrari DM, Jahn R. Endobrevin/VAMP8 mediates exocytotic release of hexosaminidase from rat basophilic leukaemia cells. FEBS Lett 2007;581:3479-84.
18. Salinas E, Quintanar-Stephano A, Cordova LE, Quintanar JL. Allergen-sensitization increases mast-cell expression of the exocytotic proteins SNAP-23 and Syntaxin 4, which are involved in histamine secretion. J Investig Allergol Clin Immunol 2008;18:366-71.
19. Puri N, Kruhlak MJ, Whiteheart SW, Roche PA. Mast cell degranulation requires N-ethylmaleimide-sensitive factor-mediated SNARE disassembly. J Immunol 2003;171:5345-52.
20. Puri N, Roche PA. Mast cells possess distinct secretory granule subsets whose exocytosis is regulated by different SNARE isoforms. Proc Natl Acad Sci U S A 2008;105:2580-5.
21. Tiwari N, Wang C-C, Brochetta C et al. VAMP-8 segregates mast cell-preformed mediator exocytosis from cytokine trafficking pathways. Blood 2008;111:3665-74.
22. Puri N, Roche PA. Ternary SNARE complexes are enriched in lipid rafts during mast cell exocytosis. Traffic 2006;7:1482-94.
23. Castanotto D, Rossi JJ. The promises and pitfalls of RNA-interference-based therapeutics. Nature 2009;457:426-33.
24. Sisson TH, Castor CW. An improved method for immobilizing IgG antibodies on protein A-agarose. J Immunol Methods 1990;127: 215-20.
25. Tadakora S, Kurimoto T, Nakanashi M, Hirashima N. Munc18-2 regulates exocytotic membrane fusion positively interacting with syntaxin-3 in RBL-2H3 cells. Mol Immunol 2007;44:3427-33.
26. Sanderson MP, Gelling SJ, Rippmann JF, Schnapp A. Comparison of the anti-allergic activity of Syk inhibitors with optimized Syk siRNAs in FcεRI-activated RBL-2H3 basophilic cells. Cell Immunol 2010;262:28-34.
27. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ddCT method. Methods 2001;25:402-8.
28. Sander LE, Frank SPC, Bolat S et al. Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells. Eur J Immunol 2008;38:855-63.
29. Kalandadanond S, Coffield JA. Cleavage of intracellular substrates of Botulinum toxins A, C, and D in a mammalian target tissue. J Pharmacol Exp Ther 2001;296:749-55.
30. Schiavo G, Benfenati F, Poulain B et al. Tetanus and Botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin. Nature 1992;359:832-5.
31. Xu T, Binz T, Niemann H, Neher E. Multiple kinetic components of exocytosis distinguished by neurotoxin sensitivity. Nat Neurosci 1998;1:175-6.
32. McBride HM, Rybin V, Murphy C, Giner A, Teasdale R, Zerial M. Oligomeric complexes link Rab5 effectors with NSF and drive membrane fusion via interactions between EEA1 and syntaxin 13. Cell 1999;98:377-86.
33. Bethani I, Werner A, Kadian C, Geumann U, Jahn R, Rizzoli SO. Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking. Traffic 2009;10:1543-59.