Hydrolysis of milk lactose in a packed bed reactor system using immobilized yeast cells

Journal of Chemical Technology and Biotechnology

Volumn 86, Issue 1, 2011, Pages 42-46

  Panesar, Reeba ^a,b   Panesar, Parmjit S ^b   Singh, Ram S ^a   Kennedy, John F ^c  

^a PUNJABI UNIVERSITY   (India)

^b SANT LONGOWAL INSTITUTE OF ENGINEERING AND TECHNOLOGY   (India)

^c Institute of Advanced Science and Technology   (United Kingdom)

Author keywords

D galactosidase; Alginate gel; Immobilization; Lactose hydrolysis; Packed bed reactor; Yeast

Indexed keywords

ALGINATE GEL; GALACTOSIDASES; IMMOBILIZATION; LACTOSE HYDROLYSIS; PACKED BED REACTOR;

ALGINATE; CELL CULTURE; CELL MEMBRANES; CHEMICAL REACTORS; DAIRY PRODUCTS; ENZYMES; GELS; HYDROLYSIS; PACKED BEDS; SUGARS; YEAST;

CELL IMMOBILIZATION;

BETA GALACTOSIDASE; CALCIUM ALGINATE; LACTOSE;

ARTICLE; BIOMASS; CELL MEMBRANE PERMEABILITY; CELL PERMEABILIZATION; DAIRY PRODUCT; ENZYME ISOLATION; FLOW RATE; HYDROLYSIS; IMMOBILIZED CELL; NONHUMAN; PACKED BED REACTOR; TEMPERATURE; YEAST CELL;

EID: 78650060396     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.2481     Document Type: Article

References (30)

1. Mahoney RR, Lactose: enzymatic modification, in Advanced Dairy Chemistry, ed. by Fox PF. Chapman & Hall, London, pp. 77-125 (1997).
2. Panesar PS, Panesar R, Singh RS, Kennedy JF and Kumar H, Microbial production, immobilization and applications of β-D-galactosidase. J Chem Technol Biotechnol 81: 530-543 (2006).
3. Rusynyk RA and Still CD, Lactose intolerance. J Am Osteop Assoc 101: S10-12 (2001).
4. Law BA and Goodenough PW, Enzymes in milk and cheese production. in Enzymes in Food Processing, ed. by Tucker GA and Woods LFJ. Blackie and Sons Ltd, New York, pp. 98-127 (1991).
5. Hettwer DJ and Wang HY, Protein release from chemically permeabilized Escherichia coli. in Separation, Recovery and Purification in Biotechnology, ed. by Asenjo JA and Hong J. ACS Symposium Series 314, American Chemical Society, Wasington, DC, pp. 2-8 (1986).
6. Naglak TJ, Bettewar DJ and Wang HY, Chemical permeabilization of cells for intracellular product release. in Separation Processes in Biotechnology, ed. by Assenjo JA. Marcel Dekker, New York, pp. 177-196 (1990).
7. Lee YJ, Kim CS and Oh DK, Lactulose production by β-galactosidase in permeabilized cells of Kluyveromyces lactis. Appl Microbiol Biotechnol 64: 787-793 (2004).
8. Panesar R, Panesar PS, Singh RS, Kennedy JF and Bera MB, Production of lactose hydrolyzed milk using ethanol permeabilized yeast cells. Food Chem 101: 786-790 (2007).
9. Somkuti GA, Dominiecki ME and Steinberg DH, Permeabilization of Streptococcus thermophilus and Lactobacillus delbrueckii sub sp. bulgaricus with ethanol. Current Microbiol 36: 202-206 (1998).
10. Vignoli JA, Celligoi MAC, da Silva RSF and de Barros M, The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose. Braz Arch Biol Technol 49: 683-687 (2006).
11. Joshi MS, Gowda LR, Katwa LC and Bhat SG, Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by digitonin. Enzyme Microbiol Technol 11: 439-343 (1989).
12. Marwaha SS and Kennedy JF, Ethanol production from whey permeate by immobilized yeast cells. Enzyme Microbiol Technol 6: 18-22 (1984).
13. Miller JH, Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, New York, pp. 352 (1972).
14. Nickerson TA, Vujicic IF and Lin AY, Colorimetric estimation of lactose and its hydrolytic products. J Dairy Sci 59: 386-390 (1976).
15. Ranzi BR, Porro D, Compagno C and Martegani E, Protein and cell volume distributions during the production of beta-galactosidase in batch cultures of Kluyveromyces lactis. J Biotechnol 5: 227-231 (1987).
16. Ramirez-Matheus AO and Rivas N, Production and partial characterization of β-galactosidase from Kluyveromyces lactis grown in deproteinized whey. Archivos Latinoamericanos de Nutrition 53: 194-201 (2003).
17. Panesar R, Panesar PS, Singh RS, Kennedy JF and Bera MB, Production of lactose hydrolyzed milk using ethanol permeabilized yeast cells. Food Chem 101: 786-790 (2007).
18. Panesar R, Panesar PS, Singh RS, Kennedy JF and Bera MB, Applicability of alginate entrapped yeast cells for the production of lactose hydrolyzed milk. J Food Process Eng 30: 472-484 (2007).
19. Gupta MN, Thermostabilization of proteins. Biotechnol Appl Biochem 14: 1-11 (1991).
20. Gupta S and Gupta MN, Mechanisms of irreversible thermoinactivation and medium engineering. in Thermostability of Enzymes, ed. by Gupta MN. Springer-Verlag, Germany, p. 114 (1993).
21. ™ immobilized on cellulose beads in batch and fluidized bed modes. Process Biochem 39: 325-332 (2003).
22. Bakken AP, Hill CG Jr and Amundson CH, Hydrolysis of lactose in skim milk by immobilized β-galactosidase (Bacillus circulans). Biotechnol Bioeng 39: 408-417 (1992).
23. Santos AMP, Oliveira MG and Maugeri F, Modelling thermal stability and activity of free and immobilized enzymes as a novel tool for enzyme reactor design. Bioresource Technol 98: 3142-3148 (2007).
24. Yang S-T and Okos MR, Effects of temperature on lactose hydrolysis by immobilized β-galactosidase in plug-flow reactor. Biotechnol Bioeng 33: 873-885 (1989).
25. Ates S and Mehmetoglu U, A new method for immobilization of β-galactosidase and its utilization in a plug flow reactor. Process Biochem 32: 433-436. (1997).
26. Ovsejevi K, Grazu V and Batista-Viera F, β-galactosidase from Kluyveromyces lactis immobilized on to thiosulfinate/thiosulfonate supports for lactose hydrolysis in milk and dairy by-products. Biotechnol Technol 12: 143-148 (1998).
27. Mahoney RR, Enzymes exogenous to milk in dairy, β-D-galactosidase, in Encyclopaedia of Dairy Sciences, Vol 2, ed. by Roginski H, Fuquay JW and Fox PF. Academic Press, London, pp. 907-914 (2003).
28. Szczodrak J, Hydrolysis of lactose in whey permeate by immobilized β-galactosidase from Kluyveromyces fragilis. J Mol Catal B: Enzyme 10: 631-637 (2000).
29. Kaul R, D'Souza SF and Nadkarni GB, Bactericidal effect of hen egg white support: a step towards the use of self-sterilizing enzyme supports. Biotechnol Bioeng 25: 887-889 (1983).
30. Genari AN, Passos FV and Passos FML, Configuration of a bioreactor for milk lactose hydrolysis. J Dairy Sci 86: 2783-2789 (2003).