The liposome PCR assay is more sensitive than the Vibrio cholerae enterotoxin and Escherichia coli heat-labile enterotoxin reversed passive latex agglutination test at detecting cholera toxin in feces and water

Journal of Clinical Microbiology

Volumn 48, Issue 12, 2010, Pages 4620-4622

  Evers, David L ^a,b   He, Junkun ^a,b   Mason, Jeffrey T ^a   O'Leary, Timothy J ^b  

^a ARMED FORCES INSTITUTE OF PATHOLOGY   (United States)

^b DEPARTMENT OF VETERANS AFFAIRS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLERA TOXIN; ESCHERICHIA COLI ENTEROTOXIN; LIPOSOME;

AGGLUTINATION TEST; ARTICLE; CONTROLLED STUDY; FECES ANALYSIS; HUMAN; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; WATER ANALYSIS;

BACTERIAL TOXINS; CHOLERA TOXIN; ENTEROTOXINS; ESCHERICHIA COLI PROTEINS; FECES; HUMANS; IMMUNOASSAY; LATEX FIXATION TESTS; LIPOSOMES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; WATER;

ESCHERICHIA COLI; VIBRIO CHOLERAE;

EID: 78650050669     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02019-10     Document Type: Article

References (13)

1. Almeida, R. J., F. W. Hickman-Brenner, E. G. Sowers, N. D. Puhr, J. J. Farmer III, and I. K. Wachsmuth. 1990. Comparison of a latex agglutination assay and an enzyme-linked immunosorbent assay for detecting cholera toxin. J. Clin. Microbiol. 28:128-130.
2. Aziz, K. M. S., and W. H. Mosley. 1972. Quantitative studies of toxin in the stools of jejunal aspirates of patients with cholera. J. Infect. Dis. 125:36-44.
3. Food and Drug Administration. 2001. Guidance for industry: bioanalytical method validation. U.S. Department of Health and Human Services, Washington, DC. http://www.fda.gov/downloads/Drugs/GuidanceComplianceRegulatoryInformation/ Guidances/ucm070107.pdf.
4. Harris, C. C., R. H. Yolken, H. Krokan, and I. C. Hsu. 1979. Ultrasensitive enzymatic radioimmunoassay: application to detection of cholera toxin and rotavirus. Proc. Natl. Acad. Sci. U. S. A. 76:5336-5339.
5. Honma, Y., N. Higa, T. Tsuji, and M. Iwanaga. 1995. Comparison of a reversed passive latex agglutination and a polymerase chain reaction for identification of cholera toxin producing Vibrio cholerae O1. Microbiol. Immunol. 39:59-61.
6. Israil, A., C. Balotescu, M. Damian, C. Dinu, and N. Bucurenci. 2004. Comparative study of different methods for detection of toxic and other enzymatic factors in Vibrio cholerae strains. Roum. Arch. Microbiol. Immunol. 63:63-77.
7. Jang, H., H. S. Kim, J. A. Kim, J. H. Seo, and R. Carbis. 2009. Improved purification process for cholera toxin and its application to the quantification of residual toxin in cholera vaccines. J. Microbiol. Biotechnol. 19:108-112.
8. Mason, J. T., L. Xu, Z. M. Sheng, J. He, and T. J. O'Leary. 2006. Liposome polymerase chain reaction assay for the sub-attomolar detection of cholera toxin and botulinum neurotoxin type A. Nat. Protoc. 1:2003-2011.
9. Mason, J. T., L. Xu, Z. M. Sheng, and T. J. O'Leary. 2006. A liposome-PCR assay for the ultrasensitive detection of biological toxins. Nat. Biotechnol. 24:555-557.
10. Northrup, M. A., B. Benett, D. Hadley, P. Landre, S. Lehew, J. Richards, and P. Stratton. 1998. A miniature analytical instrument for nucleic acids based on micromachined silicon reaction chambers. Anal. Chem. 70:918-922.
11. Ramamurthy, T., S. K. Bhattacharya, Y. Uesaka, K. Horigome, M. Paul, D. Sen, S. C. Pal, T. Takeda, Y. Takeda, and G. B. Nair. 1992. Evaluation of the bead enzyme-linked immunosorbent assay for detection of cholera toxin directly from stool specimens. J. Clin. Microbiol. 30:1783-1786.
12. Sack, D. A., R. B. Sack, G. B. Nair, and A. K. Siddique. 2004. Cholera. Lancet 363:223-233.
13. Varela, P., G. D. Pollevick, M. Rivas, I. Chinen, N. Binsztein, A. C. Frasch, and R. A. Ugalde. 1994. Direct detection of Vibrio cholerae in stool samples. J. Clin. Microbiol. 32:1246-1248.