High-throughput Xenopus laevis immunohistochemistry using agarose sections

Cold Spring Harbor Protocols

Volumn 5, Issue 12, 2010, Pages

  Blackiston, Douglas ^a   Vandenberg, Laura N ^a   Levin, Michael ^a  

^a TUFTS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; GLUTARALDEHYDE;

ARTICLE; AUTOFLUORESCENCE; EMBRYO CULTURE; HIGH THROUGHPUT SCREENING; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; NONHUMAN; XENOPUS LAEVIS;

XENOPUS LAEVIS;

EID: 78650042810     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5532     Document Type: Article

References (5)

1. Gerth VE, Zhou X, Vize PD. 2005. Nephrin expression and three-dimensional morphogenesis of the Xenopus pronephric glomus. Dev Dyn 233: 1131-1139.
2. Lee C, Kieserman E, Gray RS, Park TJ, Wallingford J. 2008. Whole-mount fluorescence immunocytochemistry on Xenopus embryos. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4957.
3. Levin M. 2004. A novel immunohistochemical method for evaluation of antibody specificity and detection of labile targets in biological tissue. J Biochem Biophys Methods 58: 85-96.
4. Monsoro-Burq AH. 2007. A rapid protocol for whole-mount in situ hybridization on Xenopus embryos. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4809.
5. Wallingford JB. 2010. Preparation of fixed Xenopus embryos for confocal imaging. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5426.