Epigallocatechin-3 gallate, a green tea catechin, attenuated the downregulation of the cardiac gap junction induced by high glucose in neonatal rat cardiomyocytes

Cellular Physiology and Biochemistry

Volumn 26, Issue 3, 2010, Pages 403-412

  Yu, Lu ^a   Zhao, Yanbo ^a   Fan, Youqi ^a   Wang, Min ^a   Xu, Shengjie ^a   Fu, Guosheng ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Cardiomyocytes; Epigallocatechin 3 gallate; Gap junction; High glucose

Indexed keywords

4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; CONNEXIN 40; CONNEXIN 43; CONNEXIN 45; DYE; EPIGALLOCATECHIN GALLATE; GLUCOSE; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE P38; STRESS ACTIVATED PROTEIN KINASE;

ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL TISSUE; ARTICLE; ATTENUATION; CELL FUNCTION; CELL VIABILITY; CONTROLLED STUDY; DOSE RESPONSE; DOWN REGULATION; DRUG EFFECT; GAP JUNCTION; HEART MUSCLE CELL; HEART PROTECTION; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; RAT; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

RATTUS;

EID: 78650030485     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000320564     Document Type: Article

References (38)

1. Bruzzone R, White TW, Paul DL: Connections with connexins: The molecular basis of direct intercellular signaling. Eur J Biochem 1996;238:1-27.
2. Kanno S, Saffitz JE: The role of myocardial gap junctions in electrical conduction and arrhythmogenesis. Cardiovasc Pathol 2001;10:169-177.
3. De Groot JR, Coronel R: Acute ischemia-induced gap junctional uncoupling and arrhythmogenesis. Cardiovasc Res 2004;62:323-334.
4. Vozzi C, Dupont E, Coppen SR, Yeh HI, Severs NJ: Chamber-related differences in connexin expression in the human heart. J Mol Cell Cardiol 1999;31:991-1003.
5. Kieken F, Mutsaers N, Dolmatova E, Virgil K, Wit AL, Kellezi A, Hirst-Jensen BJ, Duffy HS, Sorgen PL: Structural and molecular mechanisms of gap junction remodeling in epicardial border zone myocytes following myocardial infarction. Circ Res 2009;104:1103-1112.
6. Lin H, Ogawa K, Imanaga I, Tribulova N: Remodeling of connexin 43 in the diabetic rat heart. Mol Cell Biochem 2006;290:69-78. (Pubitemid 44473236)
7. Nygren A, Olson ML, Chen KY, Emmett T, Kargacin G, Shimoni Y: Propagation of the cardiac impulse in the diabetic rat heart: Reduced conduction reserve. J Physiol 2007;580:543-560.
8. Howarth FC, Chandler NJ, Kharche S, Tellez JO, Greener ID, Yamanushi TT, Billeter R, Boyett MR, Zhang H, Dobrzynski H: Effects of streptozotocin-induced diabetes on connexin43 mRNA and protein expression in ventricular muscle. Mol Cell Biochem 2008;319:105-114.
9. Stilli D, Lagrasta C, Berni R, Bocchi L, Savi M, Delucchi F, Graiani G, Monica M, Maestri R, Baruffi S, Rossi S, Macchi E, Musso E, Quaini F: Preservation of ventricular performance at early stages of diabetic cardiomyopathy involves changes in myocyte size, number and intercellular coupling. Basic Res Cardiol 2007;102:488-499.
10. Townsend PA, Scarabelli TM, Pasini E, Gitti G, Menegazzi M, Suzuki H, Knight RA, Latchman DS, Stephanou A: Epigallocatechin-3-gallate inhibits STAT-1 activation and protects cardiac myocytes from ischemia/reperfusion-induced apoptosis. FASEB J 2004;18:1621-1623.
11. Li HL, Huang Y, Zhang CN, Liu G, Wei YS, Wang AB, Liu YQ, Hui RT, Wei C, Williams GM, Liu DP, Liang CC: Epigallocathechin-3 gallate inhibits cardiac hypertrophy through blocking reactive oxidative species-dependent and -independent signal pathways. Free Radic Biol Med 2006;40:1756-1775.
12. Cai EP, Lin JK: Epigallocatechin gallate (EGCG) and rutin suppress the glucotoxicity through activating Irs2 and AMPK signaling in rat pancreatic beta cells. J Agric Food Chem 2009;57:9817-9827.
13. Spinella F, Rosano L, Decandia S, Di Castro V, Albini A, Elia G, Natali PG, Bagnato A: Antitumor effect of green tea polyphenol epigallocatechin-3- gallate in ovarian carcinoma cells: Evidence for the endothelin-1 as a potential target. Exp Biol Med (Maywood) 2006;231:1123-1127.
14. Takahashi H, Nomata K, Mori K, Matsuo M, Miyaguchi T, Noguchi M, Kanetake H: The preventive effect of green tea on the gap junction intercellular communication in renal epithelial cells treated with a renal carcinogen. Anticancer Res 2004;24:3757-3762.
15. Blondel B, Roijen I, Cheneval JP: Heart cells in culture: A simple method for increasing the proportion of myoblasts. Experientia 1971;27:356-358.
16. Livak KJ, Schmittgen TD: Analysis of relative gene expression data using realtime quantitative PCR and the 2(-delta delta c(t)) method. Methods 2001;25:402-408.
17. Dlugosova K, Weismann P, Bernatova I, Sotnikova R, Slezak J, Okruhlicova L: Omega-3 fatty acids and atorvastatin affect connexin 43 expression in the aorta of hereditary hypertriglyceridemic rats. Can J Physiol Pharmacol 2009;87:1074-1082.
18. Pang Y, Hunton DL, Bounelis P, Marchase RB: Hyperglycemia inhibits capacitative calcium entry and hypertrophy in neonatal cardiomyocytes. Diabetes 2002;51:3461-3467.
19. Baumgartner-Parzer SM, Wagner L, Pettermann M, Grillari J, Gessl A, Waldhausl W: High-glucose-triggered apoptosis in cultured endothelial cells. Diabetes 1995;44:1323-1327.
20. Kontogeorgis A, Li X, Kang E Y, Feig JE, Ponzio M, Kang G, Kaba RA, Wit AL, Fisher EA, Morley GE, Peters NS, Coetzee WA, Gutstein DE: Decreased connexin43 expression in the mouse heart potentiates pacing-induced remodeling of repolarizing currents. Am J Physiol Heart Circ Physiol 2008;295:H1905-1916.
21. Kizana E, Chang CY, Cingolani E, Ramirez-Correa GA, Sekar RB, Abraham MR, Ginn SL, Tung L, Alexander IE, Marban E: Gene transfer of connexin43 mutants attenuates coupling in cardiomyocytes: Novel basis for modulation of cardiac conduction by gene therapy. Circ Res 2007;100:1597-1604.
22. Okruhlicova L, Tribulova N, Misejkova M, Kucka M, Stetka R, Slezak J, Manoach M: Gap junction remodelling is involved in the susceptibility of diabetic rats to hypokalemia-induced ventricular fibrillation. Acta Histochem 2002;104:387-391.
23. Sheng R, Gu ZL, Xie ML, Zhou WX, Guo CY: Egcg inhibits cardiomyocyte apoptosis in pressure overload-induced cardiac hypertrophy and protects cardiomyocytes from oxidative stress in rats. Acta Pharmacol Sin 2007;28:191-201.
24. Chen DD, Dong YG, Liu D, He JG: Epigallocatechin-3-gallate attenuates cardiac hypertrophy partly via MAPKs signals in hypertensive rats. Clin Exp Pharmacol Physiol 2009, in press.
25. Song DK, Jang Y, Kim JH, Chun KJ, Lee D, Xu Z: Polyphenol (-)-epigallocatechin gallate during ischemia limits infarct size via mitochondrial K(ATP) channel activation in isolated rat hearts. J Korean Med Sci 2010;25:380-386.
26. Lorenz M, Hellige N, Rieder P, Kinkel HT, Trimpert C, Staudt A, Felix SB, Baumann G, Stangl K, Stangl V: Positive inotropic effects of epigallocatechin-3-gallate (EGCG) involve activation of Na+/H+ and Na+/Ca2+ exchangers. Eur J Heart Fail 2008;10:439-445.
27. Campos-Toimil M, Orallo F: Effects of (-)-epigallocatechin-3-gallate in Ca2+ -permeable non-selective cation channels and voltage-operated Ca2+ channels in vascular smooth muscle cells. Life Sci 2007;80:2147-2153.
28. Jozwiak J, Dhein S: Local effects and mechanisms of antiarrhythmic peptide AAP10 in acute regional myocardial ischemia: Electrophysiological and molecular findings. Naunyn Schmiedebergs Arch Pharmacol 2008;378:459-470.
29. Zhang Y, Kakinuma Y, Ando M, Katare RG, Yamasaki F, Sugiura T, Sato T: Acetylcholine inhibits the hypoxia-induced reduction of connexin43 protein in rat cardiomyocytes. J Pharmacol Sci 2006;101:214-222.
30. Fernandes R, Girao H, Pereira P: High glucose down-regulates intercellular communication in retinal endothelial cells by enhancing degradation of connexin 43 by a proteasome-dependent mechanism. J Biol Chem 2004;279:27219-27224.
31. Sugden PH: An overview of endothelin signaling in the cardiac myocyte. J Mol Cell Cardiol 2003;35:871-886.
32. Zhang T, Yang D, Fan Y, Xie P, Li H: Epigallocatechin-3-gallate enhances ischemia/reperfusion-induced apoptosis in human umbilical vein endothelial cells via Akt and MAPK pathways. Apoptosis 2009;14:1245-1254.
33. Guan KL: The mitogen activated protein kinase signal transduction pathway: From the cell surface to the nucleus. Cell Signal 1994;6:581-589.
34. Naor Z, Benard O, Seger R: Activation of MAPK cascades by G-protein-coupled receptors: The case of gonadotropin-releasing hormone receptor. Trends Endocrinol Metab 2000;11:91-99.
35. Turner MS, Haywood GA, Andreka P, You L, Martin PE, Evans WH, Webster KA, Bishopric NH: Reversible connexin 43 dephosphorylation during hypoxia and reoxygenation is linked to cellular AT P levels. Circ Res 2004;95:726-733.
36. Doble BW, Ping P, Kardami E: The epsilon subtype of protein kinase C is required for cardiomyocyte connexin-43 phosphorylation. Circ Res 2000;86:293-301.
37. Matsuyama D, Kawahara K: Proliferation of neonatal cardiomyocytes by connexin43 knockdown via synergistic inactivation of p38 MAPK and increased expression of FGF1. Basic Res Cardiol 2009;104:631-642.
38. Salameh A, Schneider P, Muhlberg K, Hagendorff A, Dhein S, Pfeiffer D: Chronic regulation of the expression of gap junction proteins connexin40, connexin43, and connexin45 in neonatal rat cardiomyocytes. Eur J Pharmacol 2004;503:9-16.