Improvement in efficiency of microspore culture to produce doubled haploid canola (Brassica napus L.) by flow cytometry

Plant Cell, Tissue and Organ Culture

Volumn 104, Issue 1, 2011, Pages 51-59

  Takahira, J ^a   Cousin, A ^a,b   Nelson, M N ^a,b   Cowling, W A ^a,b  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^b Canola Breeders Western Australia Pty Ltd   (Australia)

Author keywords

Brassica napus; Canola; Colchicine; Doubled haploidy; Flow cytometry; Microspore culture; Oilseed rape

Indexed keywords

BRASSICA NAPUS; CANOLA; COLCHICINE; DOUBLED HAPLOIDY; MICROSPORE CULTURE; OIL SEED RAPE;

AGRICULTURAL PRODUCTS; FLOW CYTOMETRY; SOILS; SUSPENSIONS (FLUIDS);

PLANTS (BOTANY);

BRASSICA NAPUS; BRASSICA NAPUS VAR. NAPUS;

EID: 78649976034     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-010-9803-8     Document Type: Article

References (22)

1. Cousin A, Nelson MN (2009) Twinned microspore-derived embryos of canola (Brassica napus L.) are genetically identical. Plant Cell Rep 28: 831-835. doi: 10. 1007/s00299-009-0677-3.
2. Cousin A, Heel K, Cowling WA, Nelson MN (2009) An efficient high-throughput flow cytometric method for estimating DNA ploidy level in plants. Cytometry Part A 75A: 1015-1019. doi: 10. 1002/cyto. a. 20816.
3. Dolezel J, Greilhuber J, Suda J (2007) Estimation of nuclear DNA content in plants using flow cytometry. Nat Protoc 2(9): 2233-2244. doi: 10. 1038/nprot. 2007. 310.
4. Forster BP, Heberle-Bors E, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12: 368-375. doi: 10. 1016/j. tplants. 2007. 06. 007.
5. Gamborg OL, Miller RA, Ojima L (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151-158. doi: 10. 1016/0014-4827(68)90403-5.
6. Gu HH, Zhou WJ, Hagberg P (2003) High frequency spontaneous production of doubled haploid plants in microspore cultures of Brassica rapa ssp. chinensis. Euphytica 134: 239-245.
7. Gu HH, Hagberg P, Zhou WJ (2004) Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.). Plant Growth Regul 42: 137-143.
8. Hansen NJP, Andersen SB (1996) In vitro chromosome doubling potential of colchicine, oryzalin, trifluralin, and APM in Brassica napus microspore culture. Euphytica 88: 159-164. doi: 10. 1007/BF00032447.
9. Khosravi P, Kermani MJ, Nematzadeh GA, Bihamta MR, Yokoya K (2008) Role of mitotic inhibitors and genotype on chromosome doubling of Rosa. Euphytica 160: 267-275. doi: 10. 1007/s10681-007-9571-7.
10. Klíma M, Vyvadilová M, Kučera V (2008) Chromosome doubling effects of selected antimitotic agents in Brassica napus microspore culture. Czech J Genet Plant Breed 44: 30-36.
11. Kott LS, Beversdorf WD (1990) Enhanced plant regeneration from microspore-derived embryos of Brassica napus by chilling, partial desiccation and age selection. Plant Cell Tiss Organ Cult 23: 187-192. doi: 10. 1007/BF00034430.
12. Kudo N, Kimura Y (2001) Flow cytometric evidence for endopolyploidy in seedlings of some Brassica species. Theor Appl Genet 102: 104-110. doi: 10. 1007/s001220051624.
13. Lichter R (1982) Induction of haploid plants from isolated pollen of Brassica napus. Z Pflanzenphysiol 105: 427-434.
14. Nelson M, Mason A, Castello M-C, Thomson L, Yan G, Cowling W (2009) Microspore culture preferentially selects unreduced (2n) gametes from an interspecific hybrid of Brassica napus L. × Brassica carinata Braun. Theor Appl Genet 119: 497-505.
15. Roberts AR (2007) The use of bead beating to prepare suspensions of nuclei for flow cytometry from fresh leaves, herbarium leaves, petals and pollen. Cytometry Part A. 71: 1039-1044. doi: 10. 1002/cyto. a. 20486.
16. Snowdon R (2007) Cytogenetics and genome analysis in Brassica crops. Chromosome Res 15: 85-95. doi: 10. 1007/s10577-006-1105-y.
17. Weber S, Unker F, Friedt W (2005) Improved doubled haploid production protocol for Brassica napus using microspore colchicine treatment in vitro and ploidy determination by flow cytometry. Plant Breeding 124: 511-513. doi: 10. 1111/j. 1439-0523. 2005. 01114. x.
18. Xu L, Najeeb U, Tang GX, Gu HH, Zhang GQ, He Y, Zhou WJ (2007) Haploid and doubled haploid technology. In: Gupta SK (ed) Rapeseed breeding, Volume 45, Advances in Botanical Research. Academic Press/Elsevier Ltd, San Diego, pp 181-216.
19. Zhang GQ, Zhang DQ, Tang GX, He Y, Zhou WJ (2006) Plant development from microspore-derived embryos in oilseed rape as affected by chilling, desiccation and cotyledon excision. Biologia Plantarum 50: 180-186.
20. Zhao JP, Simmonds DH, Newcomb W (1996) High frequency production of doubled haploid plants of Brassica napus cv. Topas derived from colchicine-induced microspore embryogenesis without heat shock. Plant Cell Rep 15: 668-671.
21. Zhou WJ, Hagberg P, Tang GX (2002a) Increasing embryogenesis and doubling efficiency by immediate colchicine treatment of isolated microspores in spring Brassica napus. Euphytica 128: 27-34.
22. Zhou WJ, Tang GX, Hagberg P (2002b) Efficient production of doubled haploid plants by immediate colchicine treatment of isolated microspores in winter Brassica napus. Plant Growth Regul 37: 185-192. doi: 10. 1023/A: 1020561201125.