Obtaining high-resolution images of biological macromolecules by using a cryo-electron microscope with a liquid-helium cooled stage

Micron

Volumn 42, Issue 2, 2011, Pages 100-106

  Mitsuoka, Kaoru ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Cryo electron microscopy; Cryo specimen preparation; High resolution imaging; Liquid helium cooled stage; Radiation damage

Indexed keywords

ATOMIC MODELS; BIOLOGICAL MACROMOLECULE; BIOLOGICAL SPECIMENS; CRYO-ELECTRON MICROSCOPY; CRYO-SPECIMEN PREPARATION; ELECTRON CRYSTALLOGRAPHY; ELECTRON RADIATION DAMAGE; FUTURE PROSPECTS; HELIUM COOLED; HIGH RESOLUTION IMAGE; HIGH-RESOLUTION IMAGING; IRRADIATION DAMAGE; LIQUID HELIUM TEMPERATURE; MOLECULAR RESOLUTION; RECONSTRUCTION METHOD; SINGLE-PARTICLE ANALYSIS; SPECIMEN TEMPERATURE;

CRYSTALLOGRAPHY; ELECTRON BEAMS; ELECTRON MICROSCOPES; ELECTRONS; GLUCOSE; HYDRATION; IMAGE PROCESSING; LIQUID NITROGEN; LIQUIDS; MACROMOLECULES; MEDICAL IMAGING; RADIATION DAMAGE; RADIOACTIVITY; SPECIMEN PREPARATION; STRUCTURAL ANALYSIS; SUPERFLUID HELIUM;

HIGH RESOLUTION ELECTRON MICROSCOPY;

HELIUM;

CRYOELECTRON MICROSCOPY; CRYOPRESERVATION; IMAGE PROCESSING; INSTRUMENTATION; MACROMOLECULE; METHODOLOGY; REVIEW; ULTRASTRUCTURE;

CRYOELECTRON MICROSCOPY; CRYOPRESERVATION; HELIUM; IMAGE PROCESSING, COMPUTER-ASSISTED; MACROMOLECULAR SUBSTANCES;

EID: 78649902160     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micron.2010.08.006     Document Type: Review

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