Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum

Enfermedades Infecciosas y Microbiologia Clinica

Volumn 28, Issue SUPPL. 3, 2010, Pages 1818-1826

  Nakai, Hiroyuki ^a   Hachem, Maher Abou ^a   Petersen, Bent O ^b   Westphal, Yvonne ^c   Mannerstedt, Karin ^b   Baumann, Martin J ^a   Dilokpimol, Adiphol ^a   Schols, Henk A ^c   Duus, Jens Ø ^b   Svensson, Birte ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b CARLSBERG LABORATORY   (Denmark)

^c WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

d Glucose 1 phosphate; d Glucosyl 1 fluoride; Glycoside hydrolase family 94; Inverting glycosynthase reaction; Non natural oligosaccharides; Regioselectivity

Indexed keywords

ALPHA DEXTRO GLUCOSE 1 PHOSPHATE; ALPHA DEXTRO GLUCOSYL 1 FLUORIDE; CELLOBIOSE; CLOSTRIDIUM THERMOCELLUM CELLOBIOSE PHOSPHORYLASE; CLOSTRIDIUM THERMOCELLUM CELLODEXTRIN PHOSPHORYLASE; DISACCHARIDE; GLUCOSE; GLYCOSIDASE; LAMINARIBIOSE; MONOSACCHARIDE; OLIGOSACCHARIDE; SOPHOROSE; TRISACCHARIDE; UNCLASSIFIED DRUG; CELLOBIOSE PHOSPHORYLASE; CELLODEXTRIN; CELLODEXTRIN PHOSPHORYLASE; DEXTRIN; GLUCOSE 1 PHOSPHATE; PHOSPHORYLASE;

AMINO ACID SEQUENCE; ARTICLE; CARBOHYDRATE SYNTHESIS; CATALYSIS; CHEMICAL REACTION; CHEMICAL STRUCTURE; CLOSTRIDIUM THERMOCELLUM; ELECTROSPRAY MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGOMERIZATION; REVERSE PHOPHOROLYSIS; SEQUENCE ALIGNMENT; COMPLEX FORMATION; ENZYME BINDING; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SYNTHESIS; NUCLEAR MAGNETIC RESONANCE; REVERSE PHOSPHOROLYSIS; SYNTHESIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; BIOCATALYSIS; CELLOBIOSE; CELLULOSE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLOSTRIDIUM THERMOCELLUM; DEXTRINS; ENZYME STABILITY; GLUCOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; OLIGOSACCHARIDES; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; STEREOISOMERISM; TEMPERATURE;

CELLVIBRIO GILVUS; CLOSTRIDIUM THERMOCELLUM;

EID: 78649861857     PISSN: 0213005X     EISSN: 15781852     Source Type: Journal    
DOI: 10.1016/j.biochi.2010.07.013     Document Type: Article

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