2-D in a Day: A two-dimensional gel electrophoresis work flow completed in under five hours

American Biotechnology Laboratory

Volumn 28, Issue 5, 2010, Pages 24-27

  Smejkal, Gary B ^a   Bauer, Darren J ^a  

^a UNIVERSITY OF NEW HAMPSHIRE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 78649819036     PISSN: 07493223     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. O'Farrell, P.H. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 1975, 250, 4007-21.
2. Righetti, P.G.; Stoyanov, A.V.; Zhukov, M.Y. The Proteome Revisited: Theory and Practice of All Relevant Electrophoretic Steps; Elsevier: Amsterdam, 2001; pp 295-7.
3. Rabilloud, T.; Blisnick, T.; Heller, M.; Luche S.; Aebersold, R.; Lunardi, J.; Braun-Breton, C. Electrophoresis 1999, 20,3603-10.
4. Smejkal, G.B.; Lazarev, A. Solution phase isoelectric fractionation in the multi-compartment electrolyzer: a divide and conquer strategy for the analysis of complex proteomes. Briefings in Functional Genomics and Proteomics 2005,4, 76-81.
5. Smejkal, G.B. The Coomassie chronicles: past, present, and future perspectives in Polyacrylamide gel staining. Expert Rev. Proteomics 2004, 1, 381-7.
6. Smejkal, G.B.; Li, C.; Robinson, M.H.; Lazarev, A.; Lawrence, N.; Chernokalskaya, E. Simultaneous reduction and alkylation of protein disulfides in a centrifugal ultrafiltration device prior to two-dimensional gel electrophoresis. J. Proteomic Res. 2006, 5, 983-7.
7. Wijte, D.; De Jong, A.; MoI, M.; Van Baar, B.; Heck, A. ProteomlQ Blue, a potent post-stain for the visualization and subsequent mass spectrometry based identification of fluorescent stained proteins on 2D-gels. J. Proteome Res. 2006, 5, 2033-8.