Ether-zymolyase ascospore isolation procedure: An efficient protocol for ascospores isolation in Saccharomyces cerevisiae yeast

Yeast

Volumn 27, Issue 12, 2010, Pages 999-1003

  Bahalul, Moran ^a   Kaneti, Galoz ^a   Kashi, Yechezkel ^a  

^a TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

Ether; Haploid; Offspring; Polyploid; Saccharomyces cerevisiae; Selection; Tetrad; Zymolyase

Indexed keywords

ETHER DERIVATIVE; ETHER ZYMOLYASE; UNCLASSIFIED DRUG;

ARTICLE; ASCOSPORE; CELL CULTURE; MEIOTIC PROPHASE I; NONHUMAN; PRIORITY JOURNAL; PROGENY; SACCHAROMYCES CEREVISIAE; SPOROGENESIS; TRIPLOIDY;

ETHER, ETHYL; HYDROLASES; MYCOLOGY; SACCHAROMYCES CEREVISIAE; SOIL MICROBIOLOGY; SPORES, FUNGAL;

SACCHAROMYCES CEREVISIAE;

EID: 78649785008     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1808     Document Type: Article

References (13)

1. Dawes IW, Hardie ID. 1974. Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether. Mol Gen Genet 131: 281-289.
2. Ezov TK, Boger-Nadjar E, Frenkel Z, et al. 2006. Molecular-genetic biodiversity in a natural population of the yeast Saccharomyces cerevisiae from 'Evolution Canyon': microsatellite polymorphism, ploidy and controversial sexual status. Genetics 174: 1455-1468.
3. Ezov TK, Chang SL, Frenkel Z, et al. 2009. Heterothallism in S. cerevisiae isolates from nature: effect of HO locus on the mode of reproduction. Mol. Ecol. 19: 121-131.
4. Fast D. 1973. Sporulation synchrony of Saccharomyces cerevisiae grown in various carbon sources. J Bacteriol 116: 925-930.
5. Game JC, Birrell GW, Brown JA, et al. 2003. Use of a genome-wide approach to identify new genes that control resistance of Saccharomyces cerevisiae to ionizing radiation. Radiation Research 160: 14-24.
6. Hicks JB, Herskowitz I. 1976. Interconversion of yeast mating types. I. Direct observation of the action of the homothallism (HO) gene. Genetics 83: 245-258.
7. Loidl J. 1995. Meiotic chromosome pairing in triploid and tetraploid Saccharomyces cerevisiae. Genetics 139: 1511-1520.
8. Marmiroli N, Ferri M, Puglisi PP. 1983. Involvement of mitochondrial protein synthesis in sporulation: effects of erythromycin on macromolecular synthesis, meiosis, and ascospore formation in Saccharomyces cerevisiae. J Bacteriol 154: 118-129.
9. Parenti-Castelli G, Bertoli E, Sechi AM, et al. 1974. Effect of soluble and membrane proteins upon diethyl ether extraction of aqueous phospholipid dispersions. Lipids 9: 221-228.
10. Rockmill B, Roeder SG. 1988. REDI: a yeast gene required for the segregation of chromosomes during the reductional division of meiosis. Genetics 85: 6057-6061.
11. Sherman F. 2002. Getting started with yeast. Methods Enzymol 350: 3-41.
12. Tong AHY, Evangelista M, Parsons AB, et al. 2001. Systematic genetic analysis with ordered arrays of yeast deletion mutants. Science 294: 2364-2368.
13. Zebrowskil D, Kaback DB. 2008. A simple method for isolating disomic strains of Saccharomyces cerevisiae. Yeast 25: 321-326.