Role of phospholemman phosphorylation sites in mediating kinase-dependent regulation of the Na+-K+-ATPase

American Journal of Physiology - Cell Physiology

Volumn 299, Issue 6, 2010, Pages

  Han, Fei ^a   Bossuyt, Julie ^b   Martin, Jody L ^c   Despa, Sanda ^b   Bers, Donald M ^b  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c LOYOLA UNIVERSITY CHICAGO   (United States)

Author keywords

Apparent Na affinity; FXYD; PKA; PKC

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); FORSKOLIN; MEMBRANE PROTEIN; PHORBOL DIBUTYRATE; PHOSPHOLEMMAN; UNCLASSIFIED DRUG;

ARTICLE; HELA CELL; HOMEOSTASIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; REGULATORY MECHANISM; SODIUM CELL LEVEL; SODIUM TRANSPORT;

ANIMALS; CYCLIC AMP-DEPENDENT PROTEIN KINASES; FORSKOLIN; HELA CELLS; HUMANS; MEMBRANE PROTEINS; MYOCYTES, CARDIAC; PHORBOL 12,13-DIBUTYRATE; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN KINASE C; RATS; SODIUM; SODIUM-POTASSIUM-EXCHANGING ATPASE;

RATTUS;

EID: 78649659491     PISSN: 03636143     EISSN: 15221563     Source Type: Journal    
DOI: 10.1152/ajpcell.00027.2010     Document Type: Article

References (21)

1. Bibert S, Roy S, Schaer D, Horisberger JD, Geering K. Phosphorylation of phospholemman (FXYD1) by protein kinases A and C modulates distinct Na,K-ATPase isozymes. J Biol Chem 283: 476-486, 2008.
2. Bossuyt J, Despa S, Han F, Hou Z, Robia SL, Lingrel JB, Bers DM. Isoform specificity of the Na/K-ATPase association and regulation by phospholemman. J Biol Chem 284: 26749-26757, 2009.
3. Bossuyt J, Ai X, Moorman RJ, Pogwizd SM, Bers DM. Expression and phosphorylation the Na-pump regulatory subunit phospholemman in heart failure. Circ Res 97: 558-565, 2005. (Pubitemid 41361746)
4. Crambert G, Fuzesi M, Garty H, Karlish S, Geering K. Phospholemman (FXYD1) associates with Na,K-ATPase and regulates its transport properties. Proc Natl Acad Sci USA 99: 11476-11481, 2002.
5. Crambert G, Geering K. FXYD proteins: new tissue-specific regulators of the ubiquitous Na,K-ATPase. SciSTKE 2003: RE1, 2003.
6. Despa S, Bossuyt J, Han F, Ginsburg KS, Jia LG, Kutchai H, Tucker AL, Bers DM. Phospholemman-phosphorylation mediates the beta-adrenergic effects on Na/K pump function in cardiac myocytes. Circ Res 97:252, 2005.
7. + pump rate in rat and rabbit ventricular myocytes. J Physiol 539:133-143, 2002.
8. + concentration is elevated in heart failure but Na/K pump function is unchanged. Circulation 105: 2543-2548, 2002.
9. Fuller W, Eaton P, Bell JR, Shattock MJ. Ischemia-induced phosphorylation of phospholemman directly activates rat cardiac Na/K-ATPase. FASEB J 18: 197, 2004.
10. Fuller W, Howie J, McLatchie LM, Weber RJ, Hastie CJ, Burness K, Pavlovic D, Shattock MJ. FXYD1 phosphorylation in vitro and in adult rat cardiac myocytes: threonine 69 is a novel substrate for protein kinase C. Am J Physiol Cell Physiol 296: C1346-C1355, 2009.
11. + pump function in cardiac myocytes. Circ Res 99: 1376-1383, 2006.
12. +-ATPase. Purification of alpha1/beta1/PLM complexes expressed in Pichia pastoris. J Biol Chem 281: 15790-15799, 2006.
13. Lindemann JP. Alpha-adrenergic stimulation of sarcolemmal protein phosphorylation and slow responses in intact myocardium. J Biol Chem 261: 4860-4867, 1986.
14. Mahmmoud YA, Cramb G, Maunsbach AB, Cutler CP, Meischke L, Cornelius F. Regulation of Na,K-ATPase by PLMS, the phospholemman-like protein from shark: molecular cloning, sequence, expression, cellular distribution, and functional effects of PLMS. J Biol Chem 278: 37427-37438, 2003. (Pubitemid 37175263)
15. Mahmmoud YA, Vorum H, Cornelius F. Identification of a phospholemman- like protein from shark rectal glands. Evidence for indirect regulation of Na,K-ATPase by protein kinase c via a novel member of the FXYDY family. J Biol Chem 275: 35969-35977, 2000.
16. Nestor NB, Lane LK, Blostein R. Effects of protein kinase modulators on the sodium pump activities of HeLa cells transfected with distinct alpha isoforms of Na,K-ATPase. Ann NY Acad Sci 834: 579-581, 1997.
17. Palmer CJ, Scott BT, Jones LR. Purification and complete sequence determination of the major plasma membrane substrate for cAMP-dependent protein kinase and protein kinase C in myocardium. J Biol Chem 266:11126-11130, 1991.
18. Presti CF, Jones LR, Lindemann JP. Isoproterenol-induced phosphorylation of a 15-kilodalton sarcolemmal protein in intact myocardium. J Biol Chem 260: 3860-3867, 1985.
19. Price EM, Rice DA, Lingrel JB. Structure-function studies of Na,K-ATPase. Site-directed mutagenesis of the border residues from the H1-H2 extracellular domain of the alpha subunit. J Biol Chem 265: 6638-6641, 1990.
20. Silverman BZ, Fuller W, Eaton P, Deng J, Moorman JR, Cheung JY, James AF, Shattock MJ. Serine 68 phosphorylation of phospholemman: acute isoform-specific activation of cardiac Na/K ATPase. Cardiovasc Res 65: 93-103, 2005.
21. Sweadner KJ, Rael E. The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression. Genomics 68: 41-56, 2000.