Actinohivin: Specific amino acid residues essential for anti-HIV activity

Journal of Antibiotics

Volumn 63, Issue 11, 2010, Pages 661-665

  Takahashi, Atsushi ^a   Inokoshi, Junji ^b   Tsunoda, Masaru ^a   Suzuki, Kaoru ^a   Takenaka, Akio ^a   Sekiguchi, Takeshi ^a   Omura, Satoshi ^b   Tanaka, Haruo ^a  

^a IWAKI MEISEI UNIVERSITY   (Japan)

^b KITASATO UNIVERSITY   (Japan)

Author keywords

actinohivin; anti HIV lectin; cluster effect of lectin; gp120 binding site; HMTG binding site; mannose binding site; microbicide

Indexed keywords

ACTINOHIVIN; AMINO ACID DERIVATIVE; ANTIRETROVIRUS AGENT; ASPARAGINE DERIVATIVE; ASPARTIC ACID DERIVATIVE; GLYCOPROTEIN GP 120; LECTIN; LEUCINE; TYROSINE DERIVATIVE; UNCLASSIFIED DRUG;

ANTIVIRAL ACTIVITY; ARTICLE; BINDING AFFINITY; DRUG POTENCY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN IMMUNODEFICIENCY VIRUS; NONHUMAN; PRIORITY JOURNAL; SYNCYTIUM; VIRUS TRANSMISSION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANTI-HIV AGENTS; BACTERIAL PROTEINS; BINDING SITES; ENZYME-LINKED IMMUNOSORBENT ASSAY; HIV; HIV ENVELOPE PROTEIN GP120; HIV INFECTIONS; HUMANS; MANNOSE; MUTATION; PROTEIN BINDING;

HUMAN IMMUNODEFICIENCY VIRUS;

EID: 78649648885     PISSN: 00218820     EISSN: None     Source Type: Journal    
DOI: 10.1038/ja.2010.106     Document Type: Article

References (26)

1. AIDSepidemic update (2009) Joint United Nations Programmeon HIV/AIDSand World Health Organization, (http://www.unaids.org/en/).
2. McGowan, I. Microbicides: a newfrontier in HIV prevention. Biologicals34, 241-255 (2006).
3. Leonard, C. K. et al. Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelopeglycoprotein (gp120) expressed in Chinese hamsterovarycells. J. Biol. Chem. 265, 10373-10382 (1990).
4. Rudd, P. M. & Dwek, R. A. Glycosylation: heterogeneity and the 3D structure of proteins. Crit. Rev. Biochem. Mol. Biol. 32, 1-100 (1997).
5. Burton, D. R. Avaccine for HIV type 1: the antibody perspective. Proc. Natl Acad. Sci. USA94, 10018-10023 (1997).
6. Botos, I. & Wlodawer, A. Proteins that bind high-mannose sugars of the HIVenvelope. Prog. Biophys. Mol. Biol. 88, 233-282 (2005).
7. Balzarini, J. Inhibition of HIVentry bycarbohydrate-binding proteins. AntiviralRes. 71, 237-247 (2006).
8. Matsumoto, A. et al. Longispora albida gen. nov., sp. nov., a novel genus of the family Micromonosporaceae. Int. J. Syst. Evol. Microbiol. 53, 1553-1559 (2003).
9. Chiba, H. et al. A simple screening system for anti-HIV drugs: syncytium formation assay using T-cell line tropic and macrophage tropic HIV env expressing cell lines; establishment and validation. J. Antibiot. 54, 818-826 (2001).
10. Chiba, H. et al. Actinohivin, a novel anti-HIV protein from an actinomycete that inhibits syncytium formation: isolation, characterization and biological activities. Biochem. Biophys. Res. Commun. 282, 595-601 (2001).
11. Chiba, H., Inokoshi, J., Nakashima, H., Omura, S. & Tanaka, H. Actinohivin, a novel anti-human immunodeficiency virus protein from an actinomycete, inhibits viral entry to cells by binding high-mannose type sugar chains of gp120. Biochem. Biophys. Res. Commun. 316, 203-210 (2004).
12. Tanaka, H. et al. Mechanism by which the lectin actinohivin blocks HIV infection of targetcells. Proc. Natl Acad. Sci. USA 106, 15633-15638 (2009).
13. Murzin, A. G., Lesk, A. M. & Chothia, C. β-Trefoil fold patterns of structure and sequence in Kunitz inhibitor interleukins-1ß and 1α and fibloblast growth factors. J. Mol. Biol. 223, 531-543 (1992).
14. Rutenber, E. & Robertus, J. D. Structure of ricin B-chain at 2.5 A resolution. Proteins. 10, 260-269 (1991).
15. Fujimoto, Z. et al. Crystal structure of Streptomyces olivaceoviridis E-86 ß-xylanase containing xylan-binding domain. J. Mol. Biol. 300, 575-585 (2000).
16. Fujimoto, Z. et al. Crystal structure of sugar complexes of Streptomyces olivaceoviridis E-86 xylanase: sugar-binding structure of family 13 carbohydrate binding module. J. Mol. Biol. 316, 65-78 (2002).
17. Inokoshi, J., Chiba, H., Takahashi, A., Omura, S. & Tanaka, H. Molecular cloning of actinohivin, a novel anti-HIV protein from an actinomycete, and its expression in Escherichiacoli. Biochem. Biophys. Res. Commun. 281, 1261-1265 (2001).
18. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K. & Pease, L. R. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59 (1989).
19. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685 (1970).
20. Takahashi, A., Inokoshi, J., Chiba, H., Omura, S. & Tanaka, H. Essential regions for antiviral activities of actinohivin, a sugar-binding anti-human immunodeficiency virus protein from an actinomycete. Arch. Biochem. Biophys. 437, 233-240 (2005).
21. Vyas, N. K. Atomic features of protein-carbohydrate interactions. Curr. Opin. Struct. Biol. 1, 732-740 (1991).
22. Quiocho, F. A. Probing the atomic interactions between proteins and carbohydrates. Biochem. Soc. Trans. 21, 442-448 (1993).
23. Lee, Y. C. & Lee, R. T. Carbohydrate-protein interactions: basis of glycobiology. Acc. Chem. Res. 28, 321-327 (1995).
24. Linder, M., Lindeberg, G., Reinikainen, T., Teeri, T. T. & Pettersson, G. Thedifference in affinity between two fungal cellulose-binding domains is dominated by a single amino acid substitution. FEBS Lett. 372, 96-98 (1995).
25. Reinikainen, T., Teleman, O. & Teeri, T. T. Effects of pH and high ionic strength on the adsorption and activity of native and mutated cellobiohydrolase I from Tr ichoderma reesei. Proteins. 22, 392-403 (1995).
26. Engle, A. R., Purdie, N. & Hyatt, J. A. Induced circular dichroism studyoftheaqueous solution complexation of cello-oligosaccharides and related polysaccharides with aromatic dyes. CarbohydrRes. 265, 181-195 (1994).